AB53453

FITC Anti-Neutrophil antibody [7/4]

Name: Anti-Neutrophil FITC antibody [7/4] (ab53453) Rat monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB53453
Price: 565 USD
Availability: InStock
Rating: 3 (5 reviews)

(5 Reviews)

(28 Publications)

Rat Monoclonal Neutrophil antibody - conjugated to FITC. Suitable for Flow Cyt, IHC-FoFr, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 28 publications. Immunogen corresponding to Cell preparation containing Neutrophil protein.

View Alternative Names

Ly-6B, Lymphocyte antigen 6B, Lymphocyte antigen 6B Short name=Ly-6B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Neutrophil antibody [7/4] (AB53453)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Neutrophil antibody [7/4] (AB53453)

Immunohistochemical analysis of mouse spleen cryosection tissue stained for Neutrophil using ab53453.

Flow Cytometry - FITC Anti-Neutrophil antibody [7/4] (AB53453)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-Neutrophil antibody [7/4] (AB53453)

Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti mouse neutrophil : FITC (ab53453).

Unconjugated

Anti-Neutrophil antibody [7/4]
578 PE

PE Anti-Neutrophil antibody [7/4]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7/4

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

IHC-P, IHC-Fr, Flow Cyt, IHC-FoFr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Recognizes the Ly-6B.2 antigen.

Specificity

Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Use 10μl of the working dilution to label 106 cells in 100μl. <a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab53453 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

ab53453 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophils also known as polymorphonuclear leukocytes are a type of white blood cell essential in the immune response. These cells make up around 50-70% of the total white blood cells in human circulation. Neutrophils express various surface markers including Ly6B.2 and NIMP-R14 which are important for their identification. The typical lifespan of neutrophils is short about 6 to 24 hours and they have an approximate mass of 1.4 x 10^-12 g per cell. Neutrophils primarily reside in the bloodstream but can migrate into tissues during inflammation or infection.

Biological function summary

Neutrophils play a central role in innate immunity by engulfing and destroying pathogens through phagocytosis. They exist as part of a network with other immune cells and contribute to the release of enzymes and reactive oxygen species to combat infection. Neutrophils can also secrete granules containing antimicrobial substances. Their activity is coordinated with other components of the immune system to provide an immediate response to infections and tissue injuries.

Pathways

Neutrophils participate actively in inflammatory and immune responses. They are important in pathways like the leukocyte adhesion cascade and the oxidative burst pathway. These pathways involve interactions with proteins such as integrins and NADPH oxidase which help neutrophils adhere to endothelium and generate reactive oxygen species respectively. These interactions permit neutrophils to migrate to sites of inflammation and eliminate invading pathogens efficiently.

Neutrophils relate to conditions such as chronic granulomatous disease and rheumatoid arthritis. In chronic granulomatous disease defects in components like NADPH oxidase result in an impaired ability to produce reactive oxygen species leading to recurrent infections. In rheumatoid arthritis neutrophils contribute to inflammation and joint damage through the release of enzymes and reactive oxygen intermediates. Neutrophil interactions with immune complexes in these disorders highlight the importance of their regulation in maintaining immune homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Neutrophil

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113502 PubMed41035679

2025

-altered lung microbiome is associated with enhanced anti-influenza effects via G-protein-coupled receptor120.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Hagihara,Makoto Yamashita,Tadashi Ariyoshi,Ayaka Minemura,Chika Yoshida,Seiya Higashi,Kentaro Oka,Motomichi Takahashi,Akinobu Ota,Akihiro Maenaka,Kenta Iwasaki,Jun Hirai,Yuichi Shibata,Takumi Umemura,Takeshi Mori,Hideo Kato,Nobuhiro Asai,Hiroshige Mikamo

Neurocritical care 42:945-952 PubMed39653978

2024

Small Molecule Myeloperoxidase (MPO) Inhibition Prevents Delayed Cerebral Injury (DCI) After Subarachnoid Hemorrhage (SAH) in a Murine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Safiye Limon,Aminata P Coulibaly,Jose Javier Provencio

Nature immunology 24:925-940 PubMed37188941

2023

Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Serena Gullotta,Donatella De Feo,Ekaterina Friebel,Aurora Semerano,Giulia Maria Scotti,Andrea Bergamaschi,Erica Butti,Elena Brambilla,Angela Genchi,Alessia Capotondo,Mattia Gallizioli,Simona Coviello,Marco Piccoli,Tiziana Vigo,Patrizia Della Valle,Paola Ronchi,Giancarlo Comi,Armando D'Angelo,Norma Maugeri,Luisa Roveri,Antonio Uccelli,Burkhard Becher,Gianvito Martino,Marco Bacigaluppi

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22065 PubMed34847625

2021

Galectin-9 mediates neutrophil capture and adhesion in a CD44 and β2 integrin-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Asif J Iqbal,Franziska Krautter,Isobel A Blacksell,Rachael D Wright,Shani N Austin-Williams,Mathieu-Benoit Voisin,Mohammed T Hussain,Hannah L Law,Toshiro Niki,Mitsuomi Hirashima,Michele Bombardieri,Costantino Pitzalis,Alok Tiwari,Gerard B Nash,Lucy V Norling,Dianne Cooper

Stroke 52:3374-3384 PubMed34404234

2021

Neutrophil Enzyme Myeloperoxidase Modulates Neuronal Response in a Model of Subarachnoid Hemorrhage by Venous Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Aminata P Coulibaly,Pinar Pezuk,Paul Varghese,William Gartman,Danielle Triebwasser,Joshua A Kulas,Lei Liu,Mariam Syed,Petr Tvrdik,Heather Ferris,J Javier Provencio

Immunity 54:1989-2004.e9 PubMed34363750

2021

Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Reglero-Real,Lorena Pérez-Gutiérrez,Azumi Yoshimura,Loïc Rolas,José Garrido-Mesa,Anna Barkaway,Catherine Pickworth,Rebeca S Saleeb,Maria Gonzalez-Nuñez,Shani N Austin-Williams,Dianne Cooper,Laura Vázquez-Martínez,Tao Fu,Giulia De Rossi,Matthew Golding,Mathieu-Benoit Voisin,Chantal M Boulanger,Yoshiaki Kubota,William A Muller,Sharon A Tooze,Thomas D Nightingale,Lucy Collinson,Mauro Perretti,Ezra Aksoy,Sussan Nourshargh

The Journal of clinical investigation 131: PubMed33232304

2020

Myeloid cell-targeted STAT3 inhibition sensitizes head and neck cancers to radiotherapy and T cell-mediated immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Dayson Moreira,Sagus Sampath,Haejung Won,Seok Voon White,Yu-Lin Su,Marice Alcantara,Chongkai Wang,Peter Lee,Ellie Maghami,Erminia Massarelli,Marcin Kortylewski

Nature communications 11:2488 PubMed32427863

2020

Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Lijing Kang,Huilin Yu,Xing Yang,Yuanbo Zhu,Xiaofei Bai,Ranran Wang,Yongliang Cao,Haochen Xu,Haiyu Luo,Lu Lu,Mei-Juan Shi,Yujing Tian,Wenying Fan,Bing-Qiao Zhao

Science signaling 13: PubMed32265338

2020

Endothelium-targeted deletion of the miR-15a/16-1 cluster ameliorates blood-brain barrier dysfunction in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Ma,Ping Sun,Xuejing Zhang,Milton H Hamblin,Ke-Jie Yin

Nature communications 10:5306 PubMed31757960

2019

Brain-to-cervical lymph node signaling after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Elga Esposito,Bum Ju Ahn,Jingfei Shi,Yoshihiko Nakamura,Ji Hyun Park,Emiri T Mandeville,Zhanyang Yu,Su Jing Chan,Rakhi Desai,Ayumi Hayakawa,Xunming Ji,Eng H Lo,Kazuhide Hayakawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Neutrophil antibody [7/4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Neutrophil antibody [7/4] (AB53453)

Flow Cytometry - FITC Anti-Neutrophil antibody [7/4] (AB53453)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

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