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AB80045

FITC Anti-Notch1 antibody [mN1A]

4

(3 Reviews)

|

(3 Publications)

Mouse Monoclonal Notch1 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Mouse Notch1.

View Alternative Names

Motch, Notch1, Neurogenic locus notch homolog protein 1, Notch 1, Motch A, mT14, p300

1 Images
Flow Cytometry (Intracellular) - FITC Anti-Notch1 antibody [mN1A] (AB80045)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-Notch1 antibody [mN1A] (AB80045)

ab80045 staining Notch1 in Mouse thymocytes by Intracellular flow cytometric analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mN1A

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Mouse Notch1. The exact immunogen used to generate this antibody is proprietary information.

Q01705

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p>Use 10μl for 10<sup>6</sup> cells. Membrane permeabilisation is required. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Notch1 also known as Notch-1 is a transmembrane receptor involved in cell fate decisions. It belongs to the Notch protein family and has a molecular weight of around 270 kDa. Notch1 is expressed widely particularly in tissues such as blood cells and neuronal tissue. The receptor consists of extracellular EGF-like repeats a transmembrane domain and an intracellular domain that translocates to the nucleus upon activation. Notch1 plays a critical role in intercellular communication through ligand engagement which then triggers a series of proteolytic cleavages that release the Notch intracellular domain (NICD) for nuclear translocation.
Biological function summary

Notch1 is essential in various cellular processes including differentiation proliferation and apoptosis. It functions as part of a complex signaling pathway that regulates these processes. Notch1 interacts with other elements like Jagged and Delta/Serrate/LAG-2 (DSL) family ligands to control gene expression patterns that determine cell lineage outcomes. This interaction affects the development of many systems such as the immune and nervous systems. Consequently Notch1 significantly influences the formation of organs and the maintenance of stem cell populations.

Pathways

Notch1 plays a significant role in both the Notch signaling and the Wnt signaling pathways. In the Notch signaling pathway Notch1 upon ligand binding partners with CSL (CBF1/RBP-Jκ in mammals) and Mastermind-like proteins for transcriptional regulation. This pathway interlinks with the Wnt pathway that involves proteins like β-catenin affecting the regulation of gene transcription. The interplay between Notch1 and these pathways is fundamental in determining outcomes in cell proliferation and differentiation emphasizing the interconnected nature of signaling networks.

Notch1 is associated with T-cell acute lymphoblastic leukemia and breast cancer. Altered Notch1 signaling often through gain-of-function mutations can drive oncogenesis by disrupting normal cell differentiation and promoting uncontrolled proliferation. In T-cell acute lymphoblastic leukemia aberrant activation of Notch1 leads to increased expression of target genes working closely with related proteins like c-Myc. In breast cancer dysregulation of Notch1 signaling may facilitate tumor growth and metastasis indicating its role as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
See full target information Notch1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009640

2025

CD163/CD63+ Monocyte-Derived DC Profiled in Tissue by Multi-Antigen Analysis (MAA) Discriminate Chronic Eczema and Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Windorfer,Michael Kirr,Waltraud Fröhlich,Bianca Plosnita,Christian Ostalecki,Carola Berking,Michael Sticherling,Andreas S Baur

Journal of biological engineering 7:18 PubMed23819656

2013

Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Hatano,Kevin Mercurio,Jesus Isaac Luna,Drew E Glaser,Valerie J Leppert,Kara E McCloskey

Proceedings of the National Academy of Sciences of the United States of America 96:3263-8 PubMed10077672

1999

Evidence for a physical interaction between presenilin and Notch.

Applications

Unspecified application

Species

Human

W J Ray,M Yao,P Nowotny,J Mumm,W Zhang,J Y Wu,R Kopan,A M Goate
View all publications
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