AB93579

FITC Anti-PD1 antibody [RMP1-30]

Name: FITC Anti-PD1 antibody [RMP1-30] (ab93579) | Abcam
Brand: Abcam Limited
SKU: AB93579

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(1 Publication)

Rat Monoclonal PD1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Pdcd1 protein.

View Alternative Names

CD279, Pd1, Pdcd1, Programmed cell death protein 1, Protein PD-1, mPD-1

1 Images

Flow Cytometry - FITC Anti-PD1 antibody [RMP1-30] (AB93579)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-PD1 antibody [RMP1-30] (AB93579)

Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 µg of FITC Rat IgG2b isotype control (open histogram), or 0.25 µg of ab93579 (colored histogram). Total viable cells were used for analysis.

450 violetFluor™ 450

violetFluor™ 450 Anti-PD1 antibody [RMP1-30]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RMP1-30

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Pdcd1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q02242

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "Use 0.5μg for 105-8 cells in 100μl.<a href='/en-us/products/primary-antibodies/fitc-rat-igg2b-kappa-monoclonal-eb149-10h5-isotype-control-ab136125'>ab136125</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody." } } }

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Product details

ab93579 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 10485649, PubMed : 11209085, PubMed : 11698646, PubMed : 21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed : 11015443, PubMed : 11224527, PubMed : 18287011, PubMed : 18641123, PubMed : 22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation (PubMed : 22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2 : following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed : 11698646, PubMed : 22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

See full target information Pdcd1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 172:3994-8 PubMed15034010

2004

Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).

Applications

Unspecified application

Species

Unspecified reactive species

Anja Fuchs,Marina Cella,Emanuele Giurisato,Andrey S Shaw,Marco Colonna

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-PD1 antibody [RMP1-30]

Flow Cytometry - FITC Anti-PD1 antibody [RMP1-30] (AB93579)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise