JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB86318

FITC Anti-Perforin antibody [B-D48]

4

(1 Review)

|

(2 Publications)

Mouse Monoclonal Perforin antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRF1.

View Alternative Names

PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-D48

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRF1.

P14222

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.09% Sodium azide Constituents: PBS, 5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.
Biological function summary

Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.

Pathways

Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.

Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 20889983, PubMed : 21037563, PubMed : 24558045, PubMed : 9058810, PubMed : 9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed : 9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 20889983, PubMed : 21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 20038786, PubMed : 20225066, PubMed : 24558045, PubMed : 32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed : 24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
See full target information PRF1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Immunologic research 65:1031-1045 PubMed28822075

2017

Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin.

Applications

Unspecified application

Species

Human

Kelsey Lesteberg,Jordan Orange,George Makedonas

Journal of virology 86:9351-60 PubMed22718823

2012

Expansion of polyfunctional HIV-specific T cells upon stimulation with mRNA electroporated dendritic cells in the presence of immunomodulatory drugs.

Applications

Flow Cyt

Species

Human

Brenda De Keersmaecker,Sabine D Allard,Patrick Lacor,Rik Schots,Kris Thielemans,Joeri L Aerts
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB89821

Anti-Perforin antibody [5B10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [5B10] (AB89821)

  • 0
  • 0
Primary Antibodies

AB47225

Anti-Perforin antibody [B-D48] - BSA and Azide free

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [B-D48] - BSA and Azide free (AB47225)

  • 4
  • 1
Primary Antibodies

AB47226

PE Anti-Perforin antibody [B-D48]

Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (AB47226)

  • 0
  • 0
Primary Antibodies

AB16074

Anti-Perforin antibody [CB5.4]

  • 4
  • 5

Complementary Products

Assay Kits

AB46068

Human Perforin ELISA Kit (PRF1)

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

  • 0
  • 0
Primary Antibodies

AB225472

Alexa Fluor® 488 Anti-Granzyme B antibody [EPR20129-217]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Granzyme B antibody [EPR20129-217] (AB225472)

  • 0
  • 0
Primary Antibodies

AB199837

Alexa Fluor® 647 Anti-HLA A antibody [EP1395Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HLA A antibody [EP1395Y] (AB199837)

  • 5
  • 1
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 61
Assay Kits

AB46068

Human Perforin ELISA Kit (PRF1)

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com