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Mouse Monoclonal Podoplanin antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry - FITC Anti-Podoplanin antibody [18H5] (AB205333), expandable thumbnail
  • Flow Cytometry - FITC Anti-Podoplanin antibody [18H5] (AB205333), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

The suggested use of the antibody is ≤ 0.5 μg in 100 μl volume. It is recommended that the conjugate antibody should be titrated for optimal performance for each application.

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Target data

Function

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed:14522983, PubMed:15231832, PubMed:17222411, PubMed:17616532, PubMed:18215137). Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN (PubMed:18541721). Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness (PubMed:17046996, PubMed:21376833). Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (PubMed:20962267). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (By similarity). Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix (PubMed:19268462). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed:15515019). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (PubMed:25486435). Required for normal lung cell proliferation and alveolus formation at birth (By similarity). Does not function as a water channel or as a regulator of aquaporin-type water channels (PubMed:9651190). Does not have any effect on folic acid or amino acid transport (By similarity).

Alternative names

GP36, PSEC0003, PSEC0025, PDPN, Podoplanin, Aggrus, Glycoprotein 36, PA2.26 antigen, T1-alpha, Gp36, T1A

Recommended products

Mouse Monoclonal Podoplanin antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Clone number
18H5
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

purified from cell culture supernatant

Storage

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Podoplanin also known as gp36 and PDPN is a small transmembrane protein weighing approximately 36 kDa. It mainly expresses in lymphatic endothelial cells podocytes and various other tissues like the lung and kidney. It functions as a marker in mesothelial cells and is noticeable in histochemical studies such as mesothelioma using podoplanin staining in immunohistochemistry (IHC). Podoplanin emerges as an important tool in identifying lymphatic vessels and has significant roles in the development of certain tissues.

Biological function summary

The protein participates in maintaining the integrity of cell structures and lymphangiogenesis. This protein does not form part of a larger complex but actively interacts with different molecules. In lymphoid tissues podoplanin contributes to the proper formation of lymphatic channels and assists in platelet aggregation. These actions are pivotal in wound healing and protecting the epithelial cell layers in multiple organs.

Pathways

Podoplanin impacts the platelet activation pathway and the Lymphatic Vessel Development pathway. Its interaction with CLEC-2 (C-type lectin-like receptor 2) triggers downstream signaling leading to changes in platelet morphology and actin cytoskeleton reorganization. Podoplanin’s activation of these pathways links it to key processes that include cell migration and tissue homeostasis TGF-beta interaction also represents a significant relationship within these pathways.

Associated diseases and disorders

Podoplanin has correlations with conditions like cancer specifically mesothelioma and squamous cell carcinoma. Dysregulation of podoplanin and its pathways may contribute to tumor progression and metastasis. In mesothelioma podoplanin interacts with other proteins like E-cadherin influencing cancer cell migration and adhesion. Its role in disease states highlights the importance of podoplanin IHC as a diagnostic tool aiding in the better understanding and identification of disease pathology.

Product promise

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2 product images

  • Flow Cytometry - FITC Anti-Podoplanin antibody [18H5] (ab205333), expandable thumbnail

    Flow Cytometry - FITC Anti-Podoplanin antibody [18H5] (ab205333)

    Flow cytometric analysis of primary Human dermal lymphatic endothelial cells (HDLEC) labeling Podoplanin with ab205333 at 0.5 μg in 100 μl dilution (lower panel) compared to negative control cells containing no primary antibody (upper panel) or conjugated secondary antibody alone (middle panel).

  • Flow Cytometry - FITC Anti-Podoplanin antibody [18H5] (ab205333), expandable thumbnail

    Flow Cytometry - FITC Anti-Podoplanin antibody [18H5] (ab205333)

    Flow cytometric analysis of primary Human dermal lymphatic endothelial cells (HDLEC) labeling Podoplanin with ab205333 at 0.5 μg in 100 μl dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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