FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

11 product images

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  Flow Cytometry (Intracellular) - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Overlay histogram showing HeLa cells stained with ab139565 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab139565, 1/50 dilution) for 30 min at 22°C.
  

  Isotype control antibody (black line) was rabbit IgG (monoclonal) [EPR25A] FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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  Immunocytochemistry/ Immunofluorescence - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223339 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control (FITC)) at 1/100 dilution (showing no signal) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min)

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  Flow Cytometry - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry staining of C57 BL/6 mouse bone marrow cells with FITC Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker ab323684 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody FITC Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker ab323684 or Rabbit IgG [EPR25A] FITC (1x 10⁶ in 100µl at 0.2 µg/ml (1/2500)) for 30min on ice.

  Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

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  Flow Cytometry (Intracellular) - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HEK293T-Myc-His-hSNAI1 vector labeling 6X His tag® with FITC Anti-6X His tag® [EPR20547] ab318311 (middle panel) at 1.0μg/ml (1/500) compared with a Rabbit IgG [EPR25A] FITC (left panel)
  
  Gate is set between transfected and untransfected HEK-293T cells.

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  Flow Cytometry - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry overlay histogram showing left BEND.3 positive cells and right negative NIH/3T3 stained with FITC Anti-CD105 antibody [EPR21846] ab314950 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (FITC Anti-CD105 antibody [EPR21846] ab314950) (1x 10⁶in 100µl at 1.0 µg/ml (1/500)) for 30min on ice.

  Isotype control antibody (black line) was Rabbit IgG [EPR25A] FITC used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

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  Flow Cytometry (Intracellular) - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry staining of 293T cells transfected with a Red fluorescent protein expression vector containing a Myc-His-tag (upper right) / 293T cells transfected with an empty vector containing a Myc-His-tag (lower right) with FITC Anti-RFP antibody [EPR28246-45] ab323681 or 293T cells transfected with a Red fluorescent protein expression vector containing a Myc-His-tag (upper left) / 293T cells transfected with an empty vector containing a Myc-His-tag (lower left) with Rabbit IgG monoclonal [EPR25A] - Isotype Control (FITC) (ab223339). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 90% methanol for 30 min. Cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (FITC Anti-RFP antibody [EPR28246-45] ab323681) (1x 10⁶ in 100µl at 1 µg/ml (1/500)) for 30min at 22°C.

  Isotype control antibody Rabbit IgG monoclonal [EPR25A] - Isotype Control (FITC) (ab223339) was used at the same concentration and conditions as the primary antibody. The cells were simultaneously stained with Myc.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable cells.

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  Flow Cytometry - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry staining of C57 BL/6 mouse splenocytes with FITC Anti-NKR-P1C antibody [EPR22990-31] ab315141 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody FITC Anti-NKR-P1C antibody [EPR22990-31] ab315141 or Rabbit IgG [EPR25A] FITC (1x 10⁶in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD19.

  Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

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  Flow Cytometry - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry staining of C57 BL/6 mouse splenocytes with FITC Anti-CD40 antibody [EPR18005-35] ab314951 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody FITC Anti-CD40 antibody [EPR18005-35] ab314951 or Rabbit IgG [EPR25A] FITC (1x 10⁶in 100 µl at 1.0 µg/ml (1/500)) for 30min on ice. The cells were simultaneously stained with CD19.

  Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable lymphocytes.

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  Flow Cytometry - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with FITC Anti-CD40 antibody [EPR20735] ab314949 (right) or Rabbit IgG [EPR25A] FITC (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody FITC Anti-CD40 antibody [EPR20735] ab314949 or Rabbit IgG [EPR25A] FITC (1x 10⁶in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD19.

  Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable lymphocytes.

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  Flow Cytometry - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry staining of C57 BL/6 mouse splenocytes with FITC Anti-NKR-P1C antibody [EPR22990-31] ab315141 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody FITC Anti-NKR-P1C antibody [EPR22990-31] ab315141 or Rabbit IgG [EPR25A] FITC (1x 10⁶in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD49b.

  Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

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  Flow Cytometry (Intracellular) - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

  Flow cytometry overlay histogram showing RAW264.7 treated with 100ng/ml LPS for 3h, then together with 300ng/ml BFA for another 3h (green line) or untreated RAW264.7 stained with FITC Anti-TNF alpha antibody [EPR21753-109] ab323680 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (FITC Anti-TNF alpha antibody [EPR21753-109] ab323680) (1x 10⁶ in 100µl at 1 µg/ml (1/500)) for 30min at 22°C.

  Isotype control antibody Rabbit IgG [EPR25A] FITC was used at the same concentration and conditions as the primary antibody (RAW264.7 treated - black line, RAW264.7 untreated - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.