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AB34764

FITC Anti-RFP antibody

5

(1 Review)

|

(6 Publications)

Rabbit Polyclonal RFP antibody - conjugated to FITC. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 6 publications. Immunogen corresponding to Recombinant Protein within Sea anenome Red fluorescent protein drFP583.

View Alternative Names

Red fluorescent protein drFP583, DsRed

1 Images
Western blot - FITC Anti-RFP antibody (AB34764)
  • WB

Supplier Data

Western blot - FITC Anti-RFP antibody (AB34764)

Lanes 1 - 2:

Western blot - HRP Anti-RFP antibody (<a href='/en-us/products/primary-antibodies/hrp-rfp-antibody-ab34767'>ab34767</a>) at 1/2500 dilution

Lanes 1 - 2:

Western blot - FITC Anti-RFP antibody (ab34764) at 1/2500 dilution

Lane 1:

HeLa cell extract at 10 µg

Lane 2:

HeLa lysate at 10 µg

Secondary

All lanes:

Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

ICC/IF, Flow Cyt, ELISA, WB

applications

Immunogen

Recombinant Protein within Sea anenome Red fluorescent protein drFP583. The exact immunogen used to generate this antibody is proprietary information.

Q9U6Y8

Specificity

Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rabbit-igg-isotype-control-ab37406'>ab37406</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

Product details

3.4 moles FITC per mole of rabbit IgG.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
affinity purification was followed by solid phase adsorption to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
See full target information Red fluorescent protein drFP583

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:252 PubMed31937752

2020

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Sidwell,Yang Liao,Alexandra L Garnham,Ajithkumar Vasanthakumar,Renee Gloury,Jonas Blume,Peggy P Teh,David Chisanga,Christoph Thelemann,Fabian de Labastida Rivera,Christian R Engwerda,Lynn Corcoran,Kohei Kometani,Tomohiro Kurosaki,Gordon K Smyth,Wei Shi,Axel Kallies

Iranian journal of basic medical sciences 22:623-630 PubMed31231489

2019

Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Del Carmen Ruelas Cinco,Bertha Ruíz Madrigal,José Alfredo Domínguez Rosales,Montserrat Maldonado González,Lucía De la Cruz Color,Sandra Margarita Ramírez Meza,José Rodrigo Torres Baranda,Erika Martínez López,Zamira Helena Hernández Nazará

Scientific reports 9:545 PubMed30679625

2019

Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function.

Applications

Unspecified application

Species

Unspecified reactive species

Vijay Saxena,James Fitch,John Ketz,Peter White,Amy Wetzel,Melinda A Chanley,John D Spencer,Brian Becknell,Keith R Pierce,Sam W Arregui,Raoul D Nelson,George J Schwartz,Victoria Velazquez,Logan A Walker,Xi Chen,Pearlly Yan,David S Hains,Andrew L Schwaderer

PloS one 8:e75225 PubMed24069395

2013

Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M Donnelly,Ambreesh Chawla,Jeanmarie Houghton,Yana Zavros

Molecular microbiology 68:1560-9 PubMed18452513

2008

PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids.

Applications

WB

Species

Unspecified reactive species

Victoria Carter,Shoichi Shimizu,Meiji Arai,Johannes T Dessens

Kidney international 73:1394-405 PubMed18385665

2008

A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1.

Applications

Unspecified application

Species

Unspecified reactive species

P G Starremans,X Li,P E Finnerty,L Guo,A Takakura,E G Neilson,J Zhou
View all publications

Product promise

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