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AB278685

FITC Anti-RPS6 (phospho S235 + S236) antibody [S6S235S236-R3A2]

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(1 Publication)

Rabbit Recombinant Monoclonal RPS6 phospho S235 + S236 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPS6 phospho S235 + S236.

View Alternative Names

OK/SW-cl.2, RPS6, Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

1 Images
Flow Cytometry - FITC Anti-RPS6 (phospho S235 + S236) antibody [S6S235S236-R3A2] (AB278685)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-RPS6 (phospho S235 + S236) antibody [S6S235S236-R3A2] (AB278685)

Flow cytometric analysis of U937 cells unstained U0126 and SB20350 treated cells (blue) or stained and treated with U0126 plus SB20350 (red) or treated with TPA plus calyculin A (green) using ab278685.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S6S235S236-R3A2

Isotype

IgG

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human RPS6 phospho S235 + S236. The exact immunogen used to generate this antibody is proprietary information.

P62753

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Complementary Products

Primary Antibodies

AB278772

PE Anti-RPS6 (phospho S240 + S244) antibody [S6RPS240244-CD10]

Recombinant

Flow Cytometry - PE Anti-RPS6 (phospho S240 + S244) antibody [S6RPS240244-CD10] (AB278772)

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Primary Antibodies

AB278620

FITC Anti-RPS6 (phospho S240 + S244) antibody [S6RPS240244-CD10]

Recombinant

Flow Cytometry - FITC Anti-RPS6 (phospho S240 + S244) antibody [S6RPS240244-CD10] (AB278620)

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Primary Antibodies

AB278731

APC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

Recombinant

Flow Cytometry - APC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278731)

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Primary Antibodies

AB278684

PE Anti-RPS6 (phospho S235 + S236) antibody [S6S235S236-R3A2]

Recombinant

Flow Cytometry - PE Anti-RPS6 (phospho S235 + S236) antibody [S6S235S236-R3A2] (AB278684)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.
Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 40S small ribosomal subunit (PubMed : 23636399, PubMed : 8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information RPS6 phospho S235 + S236
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 19:642-649 PubMed37721296

2023

Treatment with β-sitosterol ameliorates the effects of cerebral ischemia/reperfusion injury by suppressing cholesterol overload, endoplasmic reticulum stress, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuling Tang,Tao Yan,Saiying Wang,Qingqing Liu,Qi Yang,Yongqiang Zhang,Yujiao Li,Yumei Wu,Shuibing Liu,Yulong Ma,Le Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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