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AB7429

FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]

3

(1 Review)

|

(7 Publications)

Mouse Monoclonal S100A9 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human, Rabbit, Cynomolgus monkey, Baboon, Cow, Horse, Cat, Rhesus monkey, Dog, Guinea pig, Monkey, Rat, Pig samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing S100A9 + Calprotectin (S100A8/A9 complex) protein.

View Alternative Names

CAGB, CFAG, MRP14, S100A9, Protein S100-A9, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, MRP-14, p14

1 Images
Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (AB7429)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (AB7429)

ab7429 staining S100A9 + Calprotectin (FITC) in human peripheral blood granulocyte by Intracellular Flow Cytometry analysis.

  • Unconjugated

    Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]

  • Unconjugated

    Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] - Low endotoxin, Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MAC387

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Monkey, Rhesus monkey, Horse, Cow, Rabbit, Pig, Baboon, Dog, Rat, Cynomolgus monkey, Guinea pig, Cat

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Baboon": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Cat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Cow": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Cynomolgus monkey": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Dog": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Guinea pig": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Horse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Monkey": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Pig": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rabbit": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rhesus monkey": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Storage buffer
Preservative: 0.1% Sodium azide Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response (PubMed : 12626582, PubMed : 15331440, PubMed : 16258195, PubMed : 19122197, PubMed : 20103766, PubMed : 21325622, PubMed : 8423249). It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism (PubMed : 12626582, PubMed : 15331440, PubMed : 20103766). Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions (PubMed : 16258195, PubMed : 19122197, PubMed : 8423249). The intracellular functions include : facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase (PubMed : 15331440, PubMed : 21325622). Participates also in regulatory T-cell differentiation together with CD69 (PubMed : 26296369). Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX (PubMed : 15642721, PubMed : 22808130). The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities (PubMed : 19534726, PubMed : 8423249). Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration (PubMed : 15598812, PubMed : 21487906). Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER) (PubMed : 19402754). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade (PubMed : 19402754, PubMed : 22804476). Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth (PubMed : 19087201). Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3 (PubMed : 19935772). Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK (PubMed : 22363402). Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants (PubMed : 21912088, PubMed : 22489132). Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread (PubMed : 16258195). Has transnitrosylase activity; in oxidatively-modified low-densitity lipoprotein (LDL(ox))-induced S-nitrosylation of GAPDH on 'Cys-247' proposed to transfer the NO moiety from NOS2/iNOS to GAPDH via its own S-nitrosylated Cys-3 (PubMed : 25417112). The iNOS-S100A8/A9 transnitrosylase complex is proposed to also direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif (PubMed : 25417112).
See full target information S100A9

Additional targets

S100A8,S100A9

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Veterinary journal (London, England : 1997) 234:119-125 PubMed29680383

2018

Tumour-associated macrophages: Relation with progression and invasiveness, and assessment of M1/M2 macrophages in canine mammary tumours.

Applications

IHC-P

Species

Unspecified reactive species

L N Monteiro,M A Rodrigues,D A Gomes,B S Salgado,G D Cassali

Journal of tissue engineering 7:2041731415624667 PubMed26977285

2016

Biomimetic collagenous scaffold to tune inflammation by targeting macrophages.

Applications

IHC

Species

Unspecified reactive species

Francesca Taraballi,Bruna Corradetti,Silvia Minardi,Sebastian Powel,Fernando Cabrera,Jeff L Van Eps,Bradley K Weiner,Ennio Tasciotti

Journal of leukocyte biology 96:491-501 PubMed24823811

2014

Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J Burwitz,Jason S Reed,Katherine B Hammond,Merete A Ohme,Shannon L Planer,Alfred W Legasse,Adam J Ericsen,Yoram Richter,Gershon Golomb,Jonah B Sacha

Veterinary immunology and immunopathology 71:321-34 PubMed10587310

1999

The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.

Applications

Unspecified application

Species

Unspecified reactive species

M Gutierrez,F I Forster,S A McConnell,J P Cassidy,J M Pollock,D G Bryson

Histopathology 21:191-6 PubMed1505941

1992

The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function.

Applications

Unspecified application

Species

Unspecified reactive species

P Brandtzaeg,I Dale,T O Gabrielsen

Journal of clinical pathology 41:963-70 PubMed3056988

1988

Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen.

Applications

Unspecified application

Species

Unspecified reactive species

P Brandtzaeg,D B Jones,D J Flavell,M K Fagerhol

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 35:1217-26 PubMed3309045

1987

Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

D J Flavell,D B Jones,D H Wright
View all publications

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