AB23772

FITC Anti-SIRP alpha antibody [BL1H7]

Name: FITC Anti-SIRP alpha antibody [BL1H7] (ab23772) | Abcam
Brand: Abcam Limited
SKU: AB23772
Price: 535 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal SIRP alpha antibody - conjugated to FITC. Suitable for WB, Flow Cyt, IHC-Fr, IHC-P, IP and reacts with Pig samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing SIRPA protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BL1H7

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Pig

Applications

IP, WB, Flow Cyt, IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10 - 1/20", "FlowCyt-species-notes": "10μl of the suggested working dilution to 1x106 cells in 100μl", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.

Biological function summary

SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.

Pathways

SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.

SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SIRPA

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 13:e1338 PubMed37488671

2023

Integrated single-cell and spatial transcriptomic profiling reveals higher intratumour heterogeneity and epithelial-fibroblast interactions in recurrent bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Duo Shi,Zhuo Sun,Zuo-Bin Zhu,Xing Liu,Jun-Zhi Chen,Lin Hao,Jie-Fei Zhu,Kun Pang,Di Wu,Yang Dong,Yu-Fei Liu,Wei-Hua Chen,Qing Liang,Shi-Chao Zhuo,Cong-Hui Han

Veterinary immunology and immunopathology 94:77-81 PubMed12842613

2003

Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Nieves Domenech,M Pilar Rodríguez-Carreño,Purificación Filgueira,Belén Alvarez,Sonia Chamorro,Javier Domínguez

Tissue antigens 55:342-51 PubMed10852386

2000

A porcine cell surface receptor identified by monoclonal antibodies to SWC3 is a member of the signal regulatory protein family and associates with protein-tyrosine phosphatase SHP-1.

Applications

Unspecified application

Species

Unspecified reactive species

B Alvarez,C Sánchez,R Bullido,A Marina,J Lunney,F Alonso,A Ezquerra,J Domínguez

Veterinary immunology and immunopathology 73:73-81 PubMed10678400

2000

Induction of aggregation in porcine lymphoid cells by antibodies to CD46.

Applications

Unspecified application

Species

Unspecified reactive species

R Bullido,J Pérez de la Lastra,F Almazán,A Ezquerra,D Llanes,F Alonso,J Domínguez

View all publications

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Product promise

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