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AB193480

FITC Anti-TB Ag85A antibody

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(1 Publication)

Rabbit Polyclonal TB Ag85A antibody - conjugated to FITC. Suitable for WB and reacts with Recombinant full length protein - Mycobacterium tuberculosis samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mycobacterium tuberculosis CDC1551 fbpA aa 50 to C-terminus.

View Alternative Names

mpt44, MT3911, fbpA, Diacylglycerol acyltransferase/mycolyltransferase Ag85A, DGAT, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex A, Fibronectin-binding protein A, 85A, Ag85A, Fbps A, 85A, 85 A, FbpA, Ag85A, Fbp A, Mpt44, Mpt 44, Rv3804c, Antigen 85A, Antigen 85 A, Antigen 85 complex A, Secreted antigen Ag85A, Mycolyl transferase 85A, Mycolyl transferase 85 A, Fibronectin binding protein A, Mycobacterium tuberculosis antigen 85A

1 Images
Western blot - FITC Anti-TB Ag85A antibody (AB193480)
  • WB

Supplier Data

Western blot - FITC Anti-TB Ag85A antibody (AB193480)

All lanes:

Western blot - FITC Anti-TB Ag85A antibody (ab193480) at 2 µg/mL

Lane 1:

Recombinant Mycobacterium tuberculosis TB Ag85A protein at 1 µg

Lane 2:

Recombinant Mycobacterium tuberculosis TB Ag85A protein at 10 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mycobacterium tuberculosis

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Mycobacterium tuberculosis CDC1551 fbpA aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P9WQP2

Specificity

Reacts with Mycobacterium tuberculosis.

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Proteins & Peptides

AB124604

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mycobacterium tuberculosis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Mycobacterium tuberculosis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Precipitation Caprylic Acid and Ammonium Sulphate
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TB Ag85A also known as Antigen 85A is an important protein in Mycobacterium tuberculosis. It has a molecular mass of approximately 30-32 kDa. TB Ag85A is part of the antigen 85 complex which consists of proteins Ag85A Ag85B and Ag85C. This protein actively participates in mycobacterial cell wall synthesis. It catalyzes the transfer of mycolic acids to the cell wall arabinogalactan contributing to the bacteria's protective barrier. Expression occurs heavily in the cell envelope of Mycobacterium tuberculosis enabling vital interactions with the host's immune cells.
Biological function summary

TB Ag85A plays a significant role in the survival and virulence of Mycobacterium tuberculosis within host macrophages. It is part of the antigen 85 complex an important element in the synthesis of the mycobacterial cell envelope. This complex facilitates the interconversion of trehalose monomycolate and trehalose dimycolate critical for granuloma formation. The antigen 85 complex interferes with the host's immune response aiding the persistence of the bacterium. The synthesis and secretion of TB Ag85A by Mycobacterium tuberculosis allow it to adjust and survive hostile intra-host environments.

Pathways

TB Ag85A participates in the lipid biosynthesis pathway integral to cell envelope formation. This pathway plays a fundamental role in maintaining mycobacterial cell wall integrity. TB Ag85A is linked with proteins like Ag85B and Ag85C which are components of the same antigen 85 complex. These proteins work together within the pathway contributing to the robust defense system of Mycobacterium tuberculosis which complicates treatment and eradication efforts.

TB Ag85A is closely connected to tuberculosis a severe infectious disease. The protein's role in cell wall synthesis and immune evasion links it to the pathogen's ability to persist in the host. TB Ag85A's interactions with host immune defenses allow Mycobacterium tuberculosis to survive and proliferate in the lungs. Additionally studies propose TB Ag85A as a potential target for developing vaccines against tuberculosis given its role in infection and immune evasion. Researchers are exploring its connections to Ag85B and Ag85C for novel therapeutic interventions in tuberculosis management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor (By similarity).
See full target information fbpA

Additional targets

TB Ag85A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 29:709-721 PubMed34621049

2021

Negative feedback of SNRK to circ-SNRK regulates cardiac function post-myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Yan Wang,Xiao-Xiao Liu,Yun-Fei Deng
View all publications
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Product promise

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