AB171110

FITC Anti-TCR gamma + TCR delta antibody [5A6.E91]

Name: Anti-TCR gamma + TCR delta FITC antibody [5A6.E91] (ab171110) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB171110

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(3 Publications)

Mouse Monoclonal TRDC antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Human TRDC.

View Alternative Names

T cell receptor delta constant, TRDC

2 Images

Flow Cytometry - FITC Anti-TCR gamma + TCR delta antibody [5A6.E91] (AB171110)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-TCR gamma + TCR delta antibody [5A6.E91] (AB171110)

Flow cytometry analysis of TCR gamma + TCR delta showing positive staining in the membrane of peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected and combined with a hydrophilic polysaccharide then centrifuged. Samples transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml and 50 ul of isotype control and ab171110 at 1 : 20 added. Cells were incubated for 30 min at 4°C and washed with a cell buffer and incubated with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-TCR gamma + TCR delta antibody [5A6.E91] (AB171110)

Flow cytometry analysis of TCR gamma + TCR delta showing positive staining in the membrane of peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected and combined with a hydrophilic polysaccharide then centrifuged. Samples were transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml and 50 ul of isotype control and ab171110 at 1 : 20 added. Cells were incubated for 30 min at 4°C and washed with a cell buffer and incubated with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5A6.E91

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human TRDC. The exact immunogen used to generate this antibody is proprietary information.

B7Z8K6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: Glycerol (glycerin, glycerine), PBS, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCR gamma + TCR delta also known as gamma delta T-cell receptor is a part of the T-cell receptor (TCR) family. It has alternative names like gammadelta TCR or simply TCRδ/γ. The gamma and delta chains form a heterodimer that is part of the cell surface receptor complex. This complex is important for ligand recognition and immune response. The mass of the TCR gamma and TCR delta chains is not specified but they form between ZAP-70 and the CD3 complex on the surface of a subset of T-cells. These receptors are mainly expressed on gamma delta T-cells which are a minor population of T-cells in peripheral blood but more abundant in mucosal tissues and epithelial layers.

Biological function summary

TCR gamma and TCR delta are involved in recognizing antigens without presentation by major histocompatibility complex (MHC) molecules. The receptor complex allows these cells to respond to a diverse range of non-peptide antigens mostly derived from microbial origin including phosphoantigens and lipids. This enables gamma delta T-cells to bridge innate and adaptive immunity. They do not always require antigen processing and can recognize antigens directly contributing efficiently to immune surveillance and early response to infections and cellular stress.

Pathways

TCR gamma and TCR delta are important in the signaling networks that mediate immune responses. They are involved in pathways such as the nuclear factor of activated T-cells (NFAT) and mitogen-activated protein kinase (MAPK) pathways. These pathways promote the activation proliferation and differentiation of T-cells upon antigen recognition. The TCR complex including CD3 plays a role in these signal transduction events and ZAP-70 a protein tyrosine kinase is key for initiating these pathways upon TCR activation.

Mutations or dysregulation in TCR gamma and TCR delta can lead to immune-related conditions. For example abnormalities in these receptors have been linked to autoimmune diseases such as celiac disease and multiple sclerosis. Their role in these disorders is related to faulty recognition and response to autoantigens leading to inappropriate immune responses. Additionally gamma delta T-cells and their receptors are of interest in cancer research as they participate in tumor immunosurveillance. Connections with molecules like CD3 and ZAP-70 facilitate ongoing exploration for therapeutic interventions targeting immune-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of T cell receptor (TR) delta chain that participates in the antigen recognition (PubMed : 24600447). Gamma-delta TRs recognize a variety of self and foreign non-peptide antigens frequently expressed at the epithelial boundaries between the host and external environment, including endogenous lipids presented by MH-like protein CD1D and phosphoantigens presented by butyrophilin-like molecule BTN3A1. Upon antigen recognition induces rapid, innate-like immune responses involved in pathogen clearance and tissue repair (PubMed : 23348415, PubMed : 28920588). Binding of gamma-delta TR complex to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in the CD3 chains by the LCK and FYN kinases, allowing the recruitment, phosphorylation, and activation of ZAP70 that facilitates phosphorylation of the scaffolding proteins LCP2 and LAT. This lead to the formation of a supramolecular signalosome that recruits the phospholipase PLCG1, resulting in calcium mobilization and ERK activation, ultimately leading to T cell expansion and differentiation into effector cells (PubMed : 25674089). Gamma-delta TRs are produced through somatic rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes. The potential diversity of gamma-delta TRs is conferred by the unique ability to rearrange (D) genes in tandem and to utilize all three reading frames. The combinatorial diversity is considerably increased by the sequence exonuclease trimming and random nucleotide (N) region additions which occur during the V-(D)-J rearrangements (PubMed : 24387714).

See full target information TRDC

Additional targets

TRGC2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 70:1213-1226 PubMed33119798

2020

B7-H3 confers resistance to Vγ9Vδ2 T cell-mediated cytotoxicity in human colon cancer cells via the STAT3/ULBP2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Lu,Yanchao Ma,Mingyuan Wang,Jin Shen,Hongya Wu,Juntao Li,Nan Gao,Yanzheng Gu,Xueguang Zhang,Guangbo Zhang,Tongguo Shi,Weichang Chen

Cancer immunology research 8:1273-1286 PubMed32847938

2020

An IL6-Adenosine Positive Feedback Loop between CD73 γδTregs and CAFs Promotes Tumor Progression in Human Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guoming Hu,Pu Cheng,Jun Pan,Shimin Wang,Qiannan Ding,Zhou Jiang,Lu Cheng,Xuan Shao,Liming Huang,Jian Huang

Oncoimmunology 6:e1353858 PubMed29147601

2017

Tumor-infiltrating γδT cells predict prognosis and adjuvant chemotherapeutic benefit in patients with gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jieti Wang,Chao Lin,He Li,Ruochen Li,Yifan Wu,Hao Liu,Heng Zhang,Hongyong He,Weijuan Zhang,Jiejie Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-TCR gamma + TCR delta antibody [5A6.E91]

Flow Cytometry - FITC Anti-TCR gamma + TCR delta antibody [5A6.E91] (AB171110)

Flow Cytometry - FITC Anti-TCR gamma + TCR delta antibody [5A6.E91] (AB171110)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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