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AB171102

FITC Anti-TCR V alpha 2 antibody [F1]

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(1 Publication)

Mouse Monoclonal TRAC antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human TRAC.

View Alternative Names

TCRA, TRAC, T cell receptor alpha chain constant

1 Images
Flow Cytometry (Intracellular) - FITC Anti-TCR V alpha 2 antibody [F1] (AB171102)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-TCR V alpha 2 antibody [F1] (AB171102)

Flow cytometry analysis of TCR V alpha 2 (F1) on TCR V alpha 2 positive MOLT16 cells (left panel) or negative control HPB-ALL cells (right panel). Equal numbers of cells were stained with a FITC labeled TCR V alpha 2 (F1) monoclonal antibody (ab171102), or were left unstained. 5 μL of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F1

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Full Length Protein corresponding to Human TRAC. The exact immunogen used to generate this antibody is proprietary information.

P01848

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "5 µL for 10^4 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The T-cell receptor (TCR) V alpha 2 is a variable segment of the TCR alpha chain. It plays an important role in recognizing antigens presented by the major histocompatibility complex (MHC) on antigen-presenting cells (APCs). TCR V alpha 2 along with the beta chain forms the full functional TCR complex critical for T-cell activation. The molecular weight of a typical TCR alpha chain is around 50-60 kDa. TCR V alpha 2 can be found on the surface of T-cells specifically expressed within various subsets of T-lymphocytes in immune system.
Biological function summary

TCR V alpha 2 is responsible for the recognition of antigenic peptides. This function is achieved through its presence as part of the TCR-CD3 complex on the T-cell surface. Upon successful engagement of the TCR with peptide-MHC complexes T-cells may become activated leading to adaptive immune responses. TCR V alpha 2 does not act alone; it interacts closely with CD3 proteins which helps transmit signals inside the T-cell. This arrangement is essential for the immunological synapse formation.

Pathways

TCR V alpha 2 participates in the T-cell receptor signaling pathway. Activation of this pathway results in T-cell proliferation differentiation and cytokine production. TCR signaling involves several key molecules including ZAP70 and LAT which facilitate downstream signaling events. Additionally TCR V alpha 2 is involved in the MAPK/ERK signaling pathway which affects various cellular responses including survival and apoptosis.

TCR V alpha 2 shows links to autoimmune diseases such as multiple sclerosis and type 1 diabetes. These conditions often involve abnormal T-cell activation and function. Studies suggest that certain TCR V alpha segments including V alpha 2 preferentially recognize autoantigens leading to pathogenic T-cell responses. During these autoimmune processes proteins like insulin (in type 1 diabetes) may play a role by interacting with TCRs inappropriately which in turn triggers the immune system to attack the body’s own tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of T cell receptor (TR) alpha chain (PubMed : 24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed : 25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed : 23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed : 15040585).
See full target information TRAC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 26:354-365 PubMed29310916

2018

Chimeric Antigen Receptor-Engineered Human Gamma Delta T Cells: Enhanced Cytotoxicity with Retention of Cross Presentation.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Capsomidis,Gabriel Benthall,Heleen H Van Acker,Jonathan Fisher,Anne M Kramer,Zarah Abeln,Yvonne Majani,Talia Gileadi,Rebecca Wallace,Kenth Gustafsson,Barry Flutter,John Anderson
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Product promise

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