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AB59711

FITC Anti-TLR2 antibody [T2.5]

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(7 Publications)

Mouse Monoclonal TLR2 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Tlr2.

View Alternative Names

CD282, TIL4, TLR2, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4

2 Images
Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (AB59711)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (AB59711)

Overlay histogram showing THP1 cells stained with ab59711 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59711, 1μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (AB59711)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-TLR2 antibody [T2.5] (AB59711)

Flow cytometric analysis of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells staining TLR2 using ab59711 at a 10 μg/mL (blue). Red and black represent the isotype control, cells only.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

T2.5

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Mouse

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Mouse Tlr2. The exact immunogen used to generate this antibody is proprietary information.

Q9QUN7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
0.2 µm filtered.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 2 (TLR2) also called CD282 is a protein that detects molecules from bacteria and fungi. It has a mass of about 90 kDa. TLR2 is expressed on the surface of immune cells such as monocytes macrophages and neutrophils. This receptor recognizes pathogen-associated molecular patterns (PAMPs) and activates the immune response.
Biological function summary

TLR2 initiates signaling pathways that lead to the production of cytokines and chemokines helping to control infection. It forms dimers with other TLRs like TLR1 or TLR6 to expand its recognition range of microbial components. TLR2's activity is important for balancing pathogen recognition and avoiding overactive immune response. On macrophages TLR2 activation enhances their phagocytic ability.

Pathways

TLR2 plays roles in innate immune signaling pathways and inflammation pathways. It contributes significantly to the NF-kB and MAPK pathways which regulate inflammation and immune response. TLR2 often cooperates with proteins like MyD88 and TRAF6 in these pathways leading to downstream activation of transcription factors and production of pro-inflammatory molecules.

TLR2 is associated with inflammatory and infectious diseases including sepsis and tuberculosis. During tuberculosis interactions between TLR2 and its ligands trigger immune responses that are important for bacterial control. Additionally TLR2’s overactivation links to sepsis by causing excessive inflammation. Researchers study TLR2 for potential therapeutic targeting in these diseases assessing how modulation can improve outcomes or reduce detrimental inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed : 17889651, PubMed : 21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed : 10426995, PubMed : 10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed : 11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed : 16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed : 16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed : 19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed : 15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2 : TLR6 : CD14 : CD36 in response to diacylated lipopeptides and TLR2 : TLR1 : CD14 in response to triacylated lipopeptides (PubMed : 16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
See full target information TLR2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:1503 PubMed36932058

2023

The architecture of transmembrane and cytoplasmic juxtamembrane regions of Toll-like receptors.

Applications

Unspecified application

Species

Unspecified reactive species

F D Kornilov,A V Shabalkina,Cong Lin,P E Volynsky,E F Kot,A L Kayushin,V A Lushpa,M V Goncharuk,A S Arseniev,S A Goncharuk,Xiaohui Wang,K S Mineev

PloS one 13:e0199211 PubMed29912993

2018

Plasmatic membrane toll-like receptor expressions in human astrocytomas.

Applications

Unspecified application

Species

Unspecified reactive species

Isabele Fattori Moretti,Daiane Gil Franco,Thais Fernanda de Almeida Galatro,Sueli Mieko Oba-Shinjo,Suely Kazue Nagahashi Marie

International journal of molecular medicine 40:1750-1758 PubMed29039449

2017

Crosstalk between TLR2 and Sphk1 in microglia in the cerebral ischemia/reperfusion-induced inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Sun,Zhaoming Ding,Shengjie Xu,Zhiqiang Su,Hulun Li

Journal of neuroinflammation 13:33 PubMed26860188

2016

Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis.

Applications

Flow Cyt

Species

Mouse

Ke Liao,Minglei Guo,Fang Niu,Lu Yang,Shannon E Callen,Shilpa Buch

BMC veterinary research 8:196 PubMed23082910

2012

Effects of over-expression of TLR2 in transgenic goats on pathogen clearance and role of up-regulation of lysozyme secretion and infiltration of inflammatory cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shoulong Deng,Kun Yu,Baolu Zhang,Yuchang Yao,Yufeng Liu,Haijuan He,Hongtao Zhang,Maosheng Cui,Juncai Fu,Zhengxing Lian,Ning Li

PloS one 7:e35994 PubMed22563430

2012

Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.

Applications

Flow Cyt

Species

Human

Carmen A Ambarus,Kim C M Santegoets,Lenny van Bon,Mark H Wenink,Paul P Tak,Timothy R D J Radstake,Dominique L P Baeten

European heart journal 33:1085-94 PubMed21998404

2011

Ischaemia-induced up-regulation of Toll-like receptor 2 in circulating monocytes in cardiogenic shock.

Applications

Flow Cyt

Species

Human

Simina Selejan,Janine Pöss,Felix Walter,Matthias Hohl,Ralf Kaiser,Andrey Kazakov,Michael Böhm,Andreas Link
View all publications
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