AB58864

FITC Anti-TLR9 antibody [5G5]

Name: FITC Anti-TLR9 antibody [5G5] (ab58864) | Abcam
Brand: Abcam Limited
SKU: AB58864
Price: 140 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal TLR9 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLR9.

View Alternative Names

CD289, UNQ5798/PRO19605, TLR9, Toll-like receptor 9

1 Images

Flow Cytometry (Intracellular) - FITC Anti-TLR9 antibody [5G5] (AB58864)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-TLR9 antibody [5G5] (AB58864)

THP1 cells were incubated with an IgG2a isotype control (A) or a-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G5

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human TLR9. The exact immunogen used to generate this antibody is proprietary information.

Q9NR96

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

0.2 µm filtered

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR9 also known as Toll-like receptor 9 is a protein that detects unmethylated CpG motifs which are common in bacterial and viral DNA. This protein weighs about 116 kDa. It mainly expresses in plasmacytoid dendritic cells B cells and in epithelial cells of various tissues. TLR9 locates in the endoplasmic reticulum and translocates to endolysosomes upon activation which is essential for its detection function.

Biological function summary

TLR9 recognizes and binds to microbial DNA triggering an immune response. It is part of the Toll-like receptor family which plays an important role in the innate immune system. TLR9 engages in the formation of complexes with other molecules such as MyD88 an adaptor protein to propagate downstream signaling. This leads to the activation of transcription factors that stimulate cytokine production promoting inflammation and defense against infections.

Pathways

TLR9 participates significantly in the NF-kB activation pathway and the type I interferon signaling cascade. During these processes TLR9 interacts with proteins like IRAK4 and TRAF6. In the NF-kB pathway TLR9's role in the early immune response is coordinated with other Toll-like receptors to detect pathogen-related patterns and initiate adaptive immunity. Its influence on type I interferon signaling facilitates antiviral defense mechanisms.

TLR9's malfunction or altered expression associates with systemic lupus erythematosus (SLE) and cancer. In SLE overactivation of TLR9 leads to autoantibody production which worsens the disease. This can involve the protein BAFF which supports B cell proliferation. In cancer TLR9 can influence tumor growth and the tumor microenvironment affecting disease progression. Its modulation is being researched for therapeutic interventions in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key component of innate and adaptive immunity (PubMed : 14716310). TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms (PubMed : 14716310). TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 14716310). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 11564765, PubMed : 17932028). Also acts via ADCY7, leading to cyclic di-AMP (c-di-AMP) synthesis and activation of the NLRP3 inflammasome (By similarity). Plays a role in defense against systemic mouse cytomegalovirus infection (By similarity). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed : 23857366).

See full target information TLR9

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:954984 PubMed36591257

2022

Comparison of different gene addition strategies to modify placental derived-mesenchymal stromal cells to produce FVIII.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu M Ramamurthy,Martin Rodriguez,Hannah C Ainsworth,Jordan Shields,Diane Meares,Colin Bishop,Andrew Farland,Carl D Langefeld,Anthony Atala,Christopher B Doering,H Trent Spencer,Christopher D Porada,Graça Almeida-Porada

Scientific reports 11:222 PubMed33420306

2021

Platelet CXCL4 mediates neutrophil extracellular traps formation in ANCA-associated vasculitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kotaro Matsumoto,Hidekata Yasuoka,Keiko Yoshimoto,Katsuya Suzuki,Tsutomu Takeuchi

Journal of the Egyptian National Cancer Institute 32:15 PubMed32372371

2020

Toll-like receptor 9 negatively related to clinical outcome of AML patients.

Applications

Unspecified application

Species

Unspecified reactive species

Waiel M A Al-Kahiry,Enas A M Dammag,Hadeel S T Abdelsalam,Hayat K Fadlallah,Mona S Owais

PloS one 13:e0203557 PubMed30256831

2018

Tunable activation of therapeutic platelet-rich plasma by pulse electric field: Differential effects on clot formation, growth factor release, and platelet morphology.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew L Frelinger,Anja J Gerrits,V Bogdan Neculaes,Thomas Gremmel,Andrew S Torres,Anthony Caiafa,Sabrina L Carmichael,Alan D Michelson

Journal of immunology (Baltimore, Md. : 1950) 197:1905-13 PubMed27474073

2016

DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum.

Applications

Unspecified application

Species

Unspecified reactive species

André A Dias,Camila O Silva,João Pedro S Santos,Leonardo R Batista-Silva,Chyntia Carolina D Acosta,Amanda N B Fontes,Roberta O Pinheiro,Flávio A Lara,Alice M Machado,José Augusto C Nery,Euzenir N Sarno,Geraldo M B Pereira,Maria Cristina V Pessolani

Molecular therapy. Methods & clinical development 2014: PubMed25279386

2014

Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells.

Applications

Flow Cyt

Species

Human

Joana S Boura,Melisa Vance,Weihong Yin,Catarina Madeira,Cláudia Lobato da Silva,Christopher D Porada,Graça Almeida-Porada

Leukemia 28:1716-24 PubMed24476765

2014

A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib.

Applications

Unspecified application

Species

Human

A Ray,Z Tian,D S Das,R L Coffman,P Richardson,D Chauhan,K C Anderson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-TLR9 antibody [5G5]

Flow Cytometry (Intracellular) - FITC Anti-TLR9 antibody [5G5] (AB58864)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Proteins & Peptides

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise