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AB210925

FITC Anti-TLR9 antibody [ABM4D70]

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(1 Publication)

Rat Monoclonal TLR9 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tlr9 aa 600-900.

View Alternative Names

CD289, Toll-like receptor 9, Tlr9

1 Images
Flow Cytometry - FITC Anti-TLR9 antibody [ABM4D70] (AB210925)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-TLR9 antibody [ABM4D70] (AB210925)

Flow cytometric analysis of mouse splenocytes labeling TLR9 with ab210925 at 0.25 μg/106 cells (red) compared with negative control cells (green). FITC-conjugated secondary antibodies were used for the analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

ABM4D70

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Mouse Tlr9 aa 600-900. The exact immunogen used to generate this antibody is proprietary information.

Q9EQU3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR9 also known as Toll-like receptor 9 is a protein that detects unmethylated CpG motifs which are common in bacterial and viral DNA. This protein weighs about 116 kDa. It mainly expresses in plasmacytoid dendritic cells B cells and in epithelial cells of various tissues. TLR9 locates in the endoplasmic reticulum and translocates to endolysosomes upon activation which is essential for its detection function.
Biological function summary

TLR9 recognizes and binds to microbial DNA triggering an immune response. It is part of the Toll-like receptor family which plays an important role in the innate immune system. TLR9 engages in the formation of complexes with other molecules such as MyD88 an adaptor protein to propagate downstream signaling. This leads to the activation of transcription factors that stimulate cytokine production promoting inflammation and defense against infections.

Pathways

TLR9 participates significantly in the NF-kB activation pathway and the type I interferon signaling cascade. During these processes TLR9 interacts with proteins like IRAK4 and TRAF6. In the NF-kB pathway TLR9's role in the early immune response is coordinated with other Toll-like receptors to detect pathogen-related patterns and initiate adaptive immunity. Its influence on type I interferon signaling facilitates antiviral defense mechanisms.

TLR9's malfunction or altered expression associates with systemic lupus erythematosus (SLE) and cancer. In SLE overactivation of TLR9 leads to autoantibody production which worsens the disease. This can involve the protein BAFF which supports B cell proliferation. In cancer TLR9 can influence tumor growth and the tumor microenvironment affecting disease progression. Its modulation is being researched for therapeutic interventions in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 31408613). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 14993594, PubMed : 17474149, PubMed : 18820679, PubMed : 18931679, PubMed : 21402738, PubMed : 25686612). Plays a role in defense against systemic mouse cytomegalovirus infection (PubMed : 14993594). Controls lymphocyte response to Helicobacter infection (PubMed : 17474149). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (By similarity).
See full target information Tlr9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Computational and mathematical methods in medicine 2022:8371492 PubMed36238484

2022

Dexmedetomidine Regulates the miR-146a-5p/NF-B Axis to Alleviate Electroconvulsive Therapy-Induced Cognitive Impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Zhou,Peipei Si,Li Wang,Huiqun Jia
View all publications
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Product promise

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