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AB237356

FITC Anti-TNF alpha antibody [EPR20972]

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(2 Publications)

Rabbit Recombinant Monoclonal TNF alpha antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images
Flow Cytometry (Intracellular) - FITC Anti-TNF alpha antibody [EPR20972] (AB237356)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-TNF alpha antibody [EPR20972] (AB237356)

Overlay histogram showing Raw264.7 cells untreated (green line) and Raw264.7 cells treated with lipopolysaccharides (LPS), 100ng/ml, 7hours, with addition of brefeldin A (BFA), 1ug/ml, for the last 3 hours (red line) stained with ab237356. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237356, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) fitc (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.

  • Carrier free

    Anti-TNF alpha antibody [EPR20972] - BSA and Azide free

  • Unconjugated

    Anti-TNF alpha antibody [EPR20972]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TNF alpha antibody [EPR20972]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TNF alpha antibody [EPR20972]

  • 578 PE

    PE Anti-TNF alpha antibody [EPR20972]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20972

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Human, Mouse, Rat

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB271939

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RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-TNF alpha antibody [EPR19147] - BSA and Azide free (AB271939)

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Complementary Products

Assay Kits

AB181421

Human TNF alpha ELISA Kit

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Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Assay Kits

AB208348

Mouse TNF alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

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Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

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Primary Antibodies

AB9485

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:540 PubMed39237942

2024

Enhanced osteogenic differentiation in 3D hydrogel scaffold via macrophage mitochondrial transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Shui Qiu,Lili Cao,Dingding Xiang,Shu Wang,Di Wang,Yiyi Qian,Xiaohua Li,Xiaoshu Zhou

Journal of gastroenterology and hepatology 37:154-163 PubMed34734434

2021

CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Li,Jihu Ding,Ying Wang,Xianling Wang,Lin Lv
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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