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AB237472

FITC Anti-VAV1 (phospho Y174) antibody [EP510Y]

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(1 Publication)

Rabbit Recombinant Monoclonal VAV phospho Y174 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

VAV, VAV1, Proto-oncogene vav

1 Images
Flow Cytometry (Intracellular) - FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB237472)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB237472)

Overlay histogram showing Jurkat cells untreated (green line) and Jurkat cells treated with Pervanadate, 1mM, 30 minutes, (red line) stained with ab237472. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237472, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 660 APC

    APC Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 578 PE

    PE Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-VAV1 (phospho Y174) antibody [EP510Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP510Y

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAV1 also known as VAV-1 is a guanine nucleotide exchange factor with a mass of approximately 95 kDa. It activates Rho family GTPases by promoting the exchange of GDP for GTP. VAV1 is mainly expressed in hematopoietic cells playing an important role in immune responses. Its structure includes several domains – Dbl homology pleckstrin homology and an SH3 domain – that contribute to its function by facilitating interactions with other proteins.
Biological function summary

VAV1 regulates the signaling processes that lead to actin cytoskeletal rearrangements cell proliferation and differentiation. It forms part of larger multiprotein complexes that transmit signals from the cell surface to the nucleus. The activity of VAV1 is important for normal lymphocyte development and activation. By interacting with surface receptors it influences downstream effects altering cellular behavior and promoting adaptive immune responses.

Pathways

VAV1 is integral to the T cell receptor (TCR) signaling pathway and the B cell receptor (BCR) pathway. It acts as a link between the receptors and the Rho family of GTPases such as Rac1 and Cdc42 mediating the transmission of signals that result in cellular responses. The coupling of VAV1 with proteins like LAT and SLP-76 strengthens the signaling cascade necessary for lymphocyte activation and proliferation.

Deregulation of VAV1 is associated with certain cancers such as leukemia and lymphoma. Changes in VAV1 expression or activity can disrupt normal immune cell signaling promoting unchecked growth and survival of malignant cells. Additionally VAV1 is linked to autoimmune diseases where its altered function can lead to inappropriate immune responses. VAV1 interactions with proteins like ZAP-70 highlight its role in these pathologies highlighting its significance as a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
See full target information VAV1 phospho Y174
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 14:20417314221139794 PubMed36949843

2023

Cell shape characteristics of human skeletal muscle cells as a predictor of myogenic competency: A new paradigm towards precision cell therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Desprez,Davide Danovi,Charles H Knowles,Richard M Day
View all publications
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Product promise

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