FITC Anti-VCAM1 antibody [EPR5047]

3 product images

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  Flow Cytometry (Intracellular) - FITC Anti-VCAM1 antibody [EPR5047] (ab223982)

  Overlay histogram showing NIH3T3 cells stained with ab223982 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223982, 1/500 dilution) for 30 min at 22°C.
  

  Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  This antibody gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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  Immunocytochemistry/ Immunofluorescence - FITC Anti-VCAM1 antibody [EPR5047] (ab223982)

  ab223982 staining VCAM1 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223982 at 1/100 dilution (shown in Green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  This product also gave a positive signal under the same testing conditions in NIH3T3 cells fixed with 4% formaldehyde (10 min).

• 

  Immunocytochemistry/ Immunofluorescence - FITC Anti-VCAM1 antibody [EPR5047] (ab223982)

  ab223982 staining VCAM1 in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223982 at 1/100 dilution (shown in Green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  This product also gave a positive signal under the same testing conditions in NIH3T3 cells fixed with 100% methanol (5 min).