AB230852

Anti-FKBP10 antibody

Name: Anti-FKBP10 antibody (ab230852) | Abcam
Brand: Abcam Limited
SKU: AB230852

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(3 Publications)

Rabbit Polyclonal FKBP10 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FKBP10 aa 550-600.

View Alternative Names

FKBP65, PSEC0056, FKBP10, Peptidyl-prolyl cis-trans isomerase FKBP10, PPIase FKBP10, 65 kDa FK506-binding protein, FK506-binding protein 10, Immunophilin FKBP65, Rotamase, 65 kDa FKBP, FKBP-65, FKBP-10

3 Images

Flow Cytometry (Intracellular) - Anti-FKBP10 antibody (AB230852)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FKBP10 antibody (AB230852)

Intracellular flow cytometric analysis of A2058 cell line labeling FKBP10 with ab230852 at 1/10 dilution (green) compared withnegative control cell line (blue). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP10 antibody (AB230852)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP10 antibody (AB230852)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for FKBP10 using ab230852 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-FKBP10 antibody (AB230852)

All lanes:

Western blot - Anti-FKBP10 antibody (ab230852) at 1/1000 dilution

Lane 1:

A2058 whole cell lysate at 35 µg

Lane 2:

A-375 (human malignant melanoma cell line) whole cell lysate at 35 µg

Predicted band size: 64 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human FKBP10 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q96AY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FKBP10 also known as FKBP65 is a protein that functions as a peptidyl-prolyl cis-trans isomerase (PPIase). This protein with a mass of approximately 65 kDa is involved in protein folding by catalyzing the isomerization of proline residues in polypeptide chains. FKBP10 is expressed in the endoplasmic reticulum of cells and it contributes to the synthesis and maturation of collagens which are essential for the structural integrity of various tissues.

Biological function summary

FKBP10 plays a critical role in ensuring proper collagen biosynthesis. It forms part of a complex involved in stabilizing the collagen triple-helix structure during its assembly in the endoplasmic reticulum. This action by FKBP10 is necessary for maintaining tissue strength and flexibility due to its involvement in cross-linking collagen fibers. This activity highlights the importance of FKBP10 in connective tissue development and maintenance.

Pathways

FKBP10 is deeply embedded in the collagen synthesis and folding processes. It interacts within the pathways that incorporate transforming growth factor-beta (TGF-β) influencing extracellular matrix formation and maintenance. FKBP10's function is linked to proteins like prolyl 4-hydroxylase which are vital for collagen modification impacting the maturation and structural stability of collagen fibrils.

FKBP10 mutations have associations with connective tissue disorders such as osteogenesis imperfecta and Ehlers-Danlos syndrome. These diseases result from defects in collagen formation and stability. FKBP10's dysfunction can lead to compromised structural properties of tissues correlating with irregularities linked to the protein HSP47 another chaperone involved in collagen processing. Understanding FKBP10's role in these diseases aids in identifying potential therapeutic targets for correcting defects in collagen biosynthesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

PPIases accelerate the folding of proteins during protein synthesis.

See full target information FKBP10

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Protein and peptide letters 31:559-568 PubMed39076089

2024

MicroRNA-605-3p Inhibited the Growth and Chemoresistance of Osteosarcoma Cells via Negatively Modulating RAF1.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Wang,Weina Li,Guohui Han,Xiangdong Bai,Jun Xie

International journal of molecular sciences 24: PubMed37511172

2023

Subcellular Expression Patterns of FKBP Prolyl Isomerase 10 (FKBP10) in Colorectal Cancer and Its Clinical Significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yating Fu,Jiahui Chen,Xianhua Ma,Wenjun Chang,Xiongbao Zhang,Yu Liu,Hao Shen,Xuefei Hu,An-Jing Ren

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2105160 PubMed35233964

2022

circREEP3 Drives Colorectal Cancer Progression via Activation of FKBP10 Transcription and Restriction of Antitumor Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Chen,Luyun He,Liangbo Zhao,Guangtan Zhang,Zhiwei Wang,Pingping Zhu,Benyu Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FKBP10 antibody

Flow Cytometry (Intracellular) - Anti-FKBP10 antibody (AB230852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP10 antibody (AB230852)

Western blot - Anti-FKBP10 antibody (AB230852)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise