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AB58072

Anti-FKBP12 antibody [1E5-A12]

5

(2 Reviews)

|

(7 Publications)

Anti-FKBP12 antibody [1E5-A12] (ab58072) is a mouse monoclonal antibody detecting FKBP12 in Western Blot. Suitable for Human.

- Trusted since 2007

View Alternative Names

FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A

1 Images
Western blot - Anti-FKBP12 antibody [1E5-A12] (AB58072)
  • WB

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Western blot - Anti-FKBP12 antibody [1E5-A12] (AB58072)

FKBP12 antibody (ab58072) at 1μg/lane + HL-60 cell lysate at 25μg/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FKBP12 antibody [1E5-A12] (ab58072)

Predicted band size: 12 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E5-A12

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FKBP1A.

P62942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-FKBP12 antibody [1E5-A12] (ab58072) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of FKBP12?
Anti-FKBP12 [1E5-A12] (ab58072) specifically detects a band for FKBP12 (UniProt: P62942) at a molecular weight of 12kDa.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.
Biological function summary

FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-Β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.

Pathways

FKBP12 is actively involved in calcium signaling and TGF-Β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-Β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.

FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruits SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
See full target information FKBP1A
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:56 PubMed40136284

2025

Topical Administration of Novel FKBP12 Ligand MP-004 Improves Retinal Function and Structure in Retinitis Pigmentosa Models.

Applications

Unspecified application

Species

Unspecified reactive species

Araceli Lara-López,Klaudia Gonzalez-Imaz,María Rodríguez-Hidalgo,Miren Sarasola-Gastesi,Leire Escudero-Arrarás,Santiago Milla-Navarro,Pedro de la Villa,Maialen Sagartzazu-Aizpurua,José Ignacio Miranda,Jesús María Aizpurua,Adolfo López de Munain,Ainara Vallejo-Illarramendi,Javier Ruiz-Ederra

Proceedings of the National Academy of Sciences of the United States of America 119:e2204083119 PubMed36095197

2022

Tissue-restricted inhibition of mTOR using chemical genetics.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas R Wassarman,Kondalarao Bankapalli,Leo J Pallanck,Kevan M Shokat

Frontiers in physiology 11:562080 PubMed33192559

2020

A Temporal Examination of Cytoplasmic Ca Levels, Sarcoplasmic Reticulum Ca Levels, and Ca -Handling-Related Proteins in Different Skeletal Muscles of Hibernating Daurian Ground Squirrels.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Wang,Jie Zhang,Xiu-Feng Ma,Hui Chang,Xin Peng,Shen-Hui Xu,Hui-Ping Wang,Yun-Fang Gao

JACC. Basic to translational science 4:509-523 PubMed31468006

2019

A Mechanism for Statin-Induced Susceptibility to Myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sabine Lotteau,Niklas Ivarsson,Zhaokang Yang,Damien Restagno,John Colyer,Philip Hopkins,Andrew Weightman,Koichi Himori,Takashi Yamada,Joseph Bruton,Derek Steele,Håkan Westerblad,Sarah Calaghan

Molecular endocrinology (Baltimore, Md.) 28:249-59 PubMed24422633

2014

Bifunctional ligands allow deliberate extrinsic reprogramming of the glucocorticoid receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas W Højfeldt,Osvaldo Cruz-Rodríguez,Yasuhiro Imaeda,Aaron R Van Dyke,James P Carolan,Anna K Mapp,Jorge A Iñiguez-Lluhí

The EMBO journal 32:666-76 PubMed23361318

2013

Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.

Applications

WB

Species

Unspecified reactive species

Johannes Buheitel,Olaf Stemmann

Biochemical and biophysical research communication 422:732-8 PubMed22618235

2012

Impact of hypoxia, simulated ischemia and reperfusion in HL-1 cells on the expression of FKBP12/FKBP12.6 and intracellular calcium dynamics.

Applications

WB

Species

Mouse

Karin Åström-Olsson,Lizhen Li,Charlotta S Olofsson,Jan Borén,Hans Öhlin,Lars Grip
View all publications
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Product promise

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