Anti-FKBP12 antibody (ab24373) is a rabbit polyclonal antibody that is used to detect FKBP12 in Western Blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with FKBP12 knockout cell line validation
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- Communications biology 6:8612023Plasmodium falciparum formins are essential for invasion and sexual stage development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie Collier,Emma Pietsch,Madeline Dans,Dawson Ling,Tatyana A Tavella,Sash Lopaticki,Danushka S Marapana,Mohini A Shibu,Dean Andrew,Snigdha Tiash,Paul J McMillan,Paul Gilson,Leann Tilley,Matthew W A DixonPubMed 37596377
- iScience 25:1051392022Dissection of the MEF2D-IRF8 transcriptional circuit dependency in acute myeloid leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca Y Pingul,Hua Huang,Qingzhou Chen,Fatemeh Alikarami,Zhen Zhang,Jun Qi,Kathrin M Bernt,Shelley L Berger,Zhendong Cao,Junwei ShiPubMed 36193052
- Cell chemical biology 29:1482-1504.e72022Target protein localization and its impact on PROTAC-mediated degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuke M Simpson,Lorraine Glennie,Abigail Brewer,Jin-Feng Zhao,Jennifer Crooks,Natalia Shpiro,Gopal P SapkotaPubMed 36075213
- PLoS pathogens 18:e10098822022Deletion of the Plasmodium falciparum exported protein PTP7 leads to Maurer's clefts vesiculation, host cell remodeling defects, and loss of surface presentation of EMP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivia M S Carmo,Gerald J Shami,Dezerae Cox,Boyin Liu,Adam J Blanch,Snigdha Tiash,Leann Tilley,Matthew W A DixonPubMed 35930605
- Nature communications 13:30552022Persister state-directed transitioning and vulnerability in melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeike Chauvistré,Batool Shannan,Sheena M Daignault-Mill,Robert J Ju,Daniel Picard,Stefanie Egetemaier,Renáta Váraljai,Christine S Gibhardt,Antonio Sechi,Farnusch Kaschani,Oliver Keminer,Samantha J Stehbens,Qin Liu,Xiangfan Yin,Kirujan Jeyakumar,Felix C E Vogel,Clemens Krepler,Vito W Rebecca,Linda Kubat,Smiths S Lueong,Jan Forster,Susanne Horn,Marc Remke,Michael Ehrmann,Annette Paschen,Jürgen C Becker,Iris Helfrich,Daniel Rauh,Markus Kaiser,Sheraz Gul,Meenhard Herlyn,Ivan Bogeski,José Neptuno Rodríguez-López,Nikolas K Haass,Dirk Schadendorf,Alexander RoeschPubMed 35650266
- Molecular cell 81:3604-3622.e102021ZMYND8-regulated IRF8 transcription axis is an acute myeloid leukemia dependency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhendong Cao,Krista A Budinich,Hua Huang,Diqiu Ren,Bin Lu,Zhen Zhang,Qingzhou Chen,Yeqiao Zhou,Yu-Han Huang,Fatemeh Alikarami,Molly C Kingsley,Alexandra K Lenard,Aoi Wakabayashi,Eugene Khandros,Will Bailis,Jun Qi,Martin P Carroll,Gerd A Blobel,Robert B Faryabi,Kathrin M Bernt,Shelley L Berger,Junwei ShiPubMed 34358447
- Nature communications 11:46872020Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBehnam Nabet et. al.PubMed 32948771
- Nature chemical biology 16:635-6432020Discovery of a selective inhibitor of doublecortin like kinase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFleur M Ferguson et. al.PubMed 32251410
- Cell 177:384-398.e112019Innate Immune Signaling Organelles Display Natural and Programmable Signaling Flexibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunhao Tan et. al.PubMed 30853218
- Nature chemical biology 14:431-4412018The dTAG system for immediate and target-specific protein degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBehnam Nabet et. al.PubMed 29581585
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Cow | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
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Target data
Function
Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruits SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Alternative names
FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A
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Anti-FKBP12 antibody (ab24373) is a rabbit polyclonal antibody that is used to detect FKBP12 in Western Blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with FKBP12 knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-FKBP12 antibody (ab24373) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), in Human, Mouse, Rat samples.
What is the molecular weight of FKBP12?
Anti-FKBP12 (ab24373) specifically detects a band for FKBP12 (UniProt: P62942) at a molecular weight of 12kDa.
Trusted by the scientific community
Anti-FKBP12 (ab24373) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-FKBP12 antibody (ab24373) has been confirmed by Western blot testing in FKBP1A Knockout HAP1 cells.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.
- Biological function summary
FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.
- Pathways
FKBP12 is actively involved in calcium signaling and TGF-β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.
- Associated diseases and disorders
FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-FKBP12 antibody (ab24373)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: FKBP1A knockout HAP1 whole cell lysate (20 μg)
Lane 3: SH-SY5Y whole cell lysate (20 μg)
Lane 4: Human brain whole tissue lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab24373 observed at 12 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab24373 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP1A knockout cells. Wild-type and FKBP1A knockout samples were subjected to SDS-PAGE. ab24373 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FKBP12 antibody (ab24373)
Predicted band size: 12 kDa
Western blot - Anti-FKBP12 antibody (ab24373)
All lanes: Western blot - Anti-FKBP12 antibody (ab24373) at 1 µg/mL
Lane 1: Human Brain Tissue Lysate at 20 µg
Lane 2: Mouse Brain Tissue Lysate at 20 µg
Lane 3: Rat Brain Tissue Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Western blot - Anti-FKBP12 antibody (ab24373)
All lanes: Western blot - Anti-FKBP12 antibody (ab24373) at 1 µg/mL
All lanes: Recombinant Human FKBP12 protein (ab56524) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Exposure time: 2min
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