Anti-FKBP12 antibody (ab2918) is a rabbit polyclonal antibody that is used to detect FKBP12 in IHC-P, ICC/IF. Suitable for Human samples.
- Over 30 publications
- Trusted since 2003
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- Cells 12:2023Assessment of Covalently Binding Warhead Compounds in the Validation of the Cytomegalovirus Nuclear Egress Complex as an Antiviral Target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Tillmanns,Sigrun Häge,Eva Maria Borst,Julia Wardin,Jan Eickhoff,Bert Klebl,Sabrina Wagner,Christina Wangen,Friedrich Hahn,Eileen Socher,Manfred MarschallPubMed 37190072
- The EMBO journal 42:e1115592023A kinesin-based approach for inducing chromosome-specific mis-segregation in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMy Anh Truong,Paula Cané-Gasull,Sippe G de Vries,Wilco Nijenhuis,René Wardenaar,Lukas C Kapitein,Floris Foijer,Susanne Ma LensPubMed 37038978
- Science advances 8:eadd73772022Impact of aging and oxidative stress on specific components of excitation contraction coupling in regulating force generation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyang Xu,Bumsoo Ahn,Holly Van RemmenPubMed 36288318
- Molecular biology of the cell 33:br162022BubR1 recruitment to the kinetochore via Bub1 enhances spindle assembly checkpoint signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnand Banerjee,Chu Chen,Lauren Humphrey,John J Tyson,Ajit P JoglekarPubMed 35767360
- eLife 11:2022Role of oxidation of excitation-contraction coupling machinery in age-dependent loss of muscle function in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaikel Dridi,Frances Forrester,Alisa Umanskaya,Wenjun Xie,Steven Reiken,Alain Lacampagne,Andrew MarksPubMed 35506650
- The Journal of biological chemistry 298:1015892022Immunosuppressive calcineurin inhibitor cyclosporine A induces proapoptotic endoplasmic reticulum stress in renal tubular cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuygu Elif Yilmaz,Karin Kirschner,Hasan Demirci,Nina Himmerkus,Sebastian Bachmann,Kerim MutigPubMed 35033536
- Current biology : CB 32:237-247.e62021Aurora B phosphorylates Bub1 to promote spindle assembly checkpoint signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBabhrubahan Roy,Simon J Y Han,Adrienne N Fontan,Soubhagyalaxmi Jema,Ajit P JoglekarPubMed 34861183
- Parasitology international 85:1024352021cAMP-dependent protein kinase regulates secretion of apical membrane antigen 1 (AMA1) in Plasmodium yoelii.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakahiro Ishizaki,Masahito Asada,Hassan Hakimi,Nattawat Chaiyawong,Yuto Kegawa,Kazuhide Yahata,Osamu KanekoPubMed 34390881
- Nature chemical biology 17:1065-10742021Selective inhibitors of mTORC1 activate 4EBP1 and suppress tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca J Lee,Jacob A Boyer,G Leslie Burnett,Arun P Thottumkara,Nidhi Tibrewal,Stacy L Wilson,Tientien Hsieh,Abby Marquez,Edward G Lorenzana,James W Evans,Laura Hulea,Gert Kiss,Hui Liu,Dong Lee,Ola Larsson,Shannon McLaughlan,Ivan Topisirovic,Zhengping Wang,Zhican Wang,Yongyuan Zhao,David Wildes,James B Aggen,Mallika Singh,Adrian L Gill,Jacqueline A M Smith,Neal RosenPubMed 34168367
- Autophagy 17:4119-41402021Modulating FKBP5/FKBP51 and autophagy lowers HTT (huntingtin) levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara J Bailus,Stephen M Scheeler,Jesse Simons,Maria A Sanchez,Kizito-Tshitoko Tshilenge,Jordi Creus-Muncunill,Swati Naphade,Alejandro Lopez-Ramirez,Ningzhe Zhang,Kuruwitage Lakshika Madushani,Stanislav Moroz,Ashley Loureiro,Katherine H Schreiber,Felix Hausch,Brian K Kennedy,Michelle E Ehrlich,Lisa M EllerbyPubMed 34024231
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FKBP1A aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P62942
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruits SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Alternative names
FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A
Recommended products
Anti-FKBP12 antibody (ab2918) is a rabbit polyclonal antibody that is used to detect FKBP12 in IHC-P, ICC/IF. Suitable for Human samples.
- Over 30 publications
- Trusted since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FKBP1A aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P62942
- Purification technique
- Affinity purification Immunogen
- Specificity
Detects FK506 binding protein 12 kDa (FKBP12).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-FKBP12 antibody (ab2918) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human samples.
Trusted by the scientific community
Anti-FKBP12 (ab2918) was first used in a scientific publication in 2003 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-FKBP12 (ab2918) has over 20 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.
- Biological function summary
FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.
- Pathways
FKBP12 is actively involved in calcium signaling and TGF-β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.
- Associated diseases and disorders
FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
Immunohistochemistry was performed on normal biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/50 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody (ab2918)
ICC/IF image of ab2918 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2918, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
Immunohistochemistry was performed on normal biopsies of deparaffinized human heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/20 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
This image is courtesy of a customer review submitted by Miss gwen lagodaImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
ab2918 at 1/1000 staining FKBP12 from human penis tissue by IHC-P. The tissue was paraformaldehyde fixed and the slides were incubated in citrate buffer for antigen retrieval and heated for 20 minutes. The tissue was incubated with ab2918 for 24 hours. The secondary antibody used was part of the DAKO ENVISON System. The picture depicts staining of nerve fibers within corporal tissue of the human penis.
This image is courtesy of a customer review submitted by Miss gwen lagodaImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
ab2918 at 1/1000 staining mouse heart tissue sections by IHC-P. The tissue was paraformaldehyde fixed, blocked and then a citrate buffer / heat mediated antigen retireival step was performed. The tissue was incubated with the antibody for 24 hours. An HRP conjugated rabbit polyclonal antibody was used as the secondary.
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