JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108420

Anti-FKBP12 antibody [EP3887]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Monoclonal FKBP12 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody [EP3887] (AB108420)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody [EP3887] (AB108420)

Immunofluorescent staining of FKBP12 in HeLa cells using ab108420 at 1/50 dilution.

Western blot - Anti-FKBP12 antibody [EP3887] (AB108420)
  • WB

Lab

Western blot - Anti-FKBP12 antibody [EP3887] (AB108420)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : FKBP12 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : SH-SY5Y whole cell lysate (20 μg)
Lane 4 : Human brain whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108420 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108420 was shown to recognize FKBP12 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP12 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. ab108420 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)

Predicted band size: 12 kDa

false

Western blot - Anti-FKBP12 antibody [EP3887] (AB108420)
  • WB

Unknown

Western blot - Anti-FKBP12 antibody [EP3887] (AB108420)

All lanes:

Western blot - Anti-FKBP12 antibody [EP3887] (ab108420) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Lane 3:

U937 lysate at 10 µg

Lane 4:

SH-SY5Y lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 12 kDa

false

  • Carrier free

    Anti-FKBP12 antibody [EP3887] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3887

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB24373

Anti-FKBP12 antibody

KO Validated

Western blot - Anti-FKBP12 antibody (AB24373)

  • 5
  • 4
Primary Antibodies

AB92459

Anti-FKBP12 antibody [EPR3888]

RabMAb|Recombinant|KO Validated

Western blot - Anti-FKBP12 antibody [EPR3888] (AB92459)

  • 4
  • 2
Primary Antibodies

AB240931

Anti-FKBP12 antibody [EPR3888] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-FKBP12 antibody [EPR3888] - BSA and Azide free (AB240931)

  • 0
  • 0
Primary Antibodies

AB58072

Anti-FKBP12 antibody [1E5-A12]

Western blot - Anti-FKBP12 antibody [1E5-A12] (AB58072)

  • 5
  • 2
Primary Antibodies

AB2918

Anti-FKBP12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (AB2918)

  • 5
  • 22

Complementary Products

Assay Kits

AB277418

Human FKBP12 ELISA kit

ELISA - Human FKBP12 ELISA kit (AB277418)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.
Biological function summary

FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.

Pathways

FKBP12 is actively involved in calcium signaling and TGF-β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.

FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruits SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
See full target information FKBP1A
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37296659

2023

RANKL Inhibition Reduces Cardiac Hypertrophy in Mice and Possibly in Children with Duchenne Muscular Dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Laetitia Marcadet,Emma Sara Juracic,Nasrin Khan,Zineb Bouredji,Hideo Yagita,Leanne M Ward,A Russell Tupling,Anteneh Argaw,Jérôme Frenette

Frontiers in cell and developmental biology 8:595253 PubMed33195272

2020

ICP6 Prevents RIP1 Activation to Hinder Necroptosis Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Hu,Guoxiang Wu,Zhaoqian Shu,Dandan Yu,Ning Nan,Feiyang Yuan,Xiaoyan Liu,Huayi Wang

Cell death and differentiation 28:251-266 PubMed32737444

2020

RIP3-mediated necroptosis is regulated by inter-filament assembly of RIP homotypic interaction motif.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Hu,Xialian Wu,Guoxiang Wu,Ning Nan,Jing Zhang,Xinxin Zhu,Yu Zhang,Zhaoqian Shu,Jia Liu,Xiaoyan Liu,Junxia Lu,Huayi Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com