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Knockout Tested Rabbit Recombinant Monoclonal FKBP12 antibody. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378), expandable thumbnail
  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378), expandable thumbnail
  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378), expandable thumbnail
  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378), expandable thumbnail
  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruits SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Alternative names

FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal FKBP12 antibody. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27367-59
Purification technique
Affinity purification Protein A
Specificity

This antibody recognizes both FKBP12/FKBP1A and FKBP12.6/FKBP1B.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.

Biological function summary

FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.

Pathways

FKBP12 is actively involved in calcium signaling and TGF-β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.

Associated diseases and disorders

FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378), expandable thumbnail

    Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody recognizes both FKBP12 and FKBP12.6.

    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containi a his tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human FKBP12 expression vector containi a His-tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human FKBP12.6 expression vector containi a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa

    Observed band size: 18 kDa

    Exposure time: 15s

  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378), expandable thumbnail

    Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.

    All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) cytoplasmic fraction at 20 µg

    Lane 2: SH-SY5Y nuclear fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa

    Observed band size: 14 kDa

    Exposure time: 81s

  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378), expandable thumbnail

    Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In Western blot, ab313378 was shown to bind specifically to FKBP12. A band was observed at 14 kDa in wild-type HAP1 cell lysates whereas loss of signal was observed in the FKBP12 knockout cell line.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

    Lane 1: Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 20 µg

    Lane 2: FKBP12 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell fresh lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Predicted band size: 11 kDa

    Observed band size: 14 kDa

    Exposure time: 180s

  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378), expandable thumbnail

    Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: A549 (PMID: 27617579).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 81 seconds

    All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 12 kDa

    Exposure time: 81s

  • Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378), expandable thumbnail

    Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 48 seconds

    All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 12 kDa

    Exposure time: 48s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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