Anti-FKBP12 antibody [EPR27367-59]

• WB

Supplier Data

Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution.

All lanes:

Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma cell line from bone marrow) cytoplasmic fraction at 20 µg

Lane 2:

SH-SY5Y nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 14 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

In Western blot, ab313378 was shown to bind specifically to FKBP12. A band was observed at 14 kDa in wild-type HAP1 cell lysates whereas loss of signal was observed in the FKBP12 knockout cell line.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

Lane 1:

Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 20 µg

Lane 2:

FKBP12 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 14 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : A549 (PMID : 27617579). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 81 seconds

All lanes:

Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 6:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 12 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 48 seconds

All lanes:

Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 12 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-FKBP12 antibody [EPR27367-59] (AB313378)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody recognizes both FKBP12 and FKBP12.6.

In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FKBP12 antibody [EPR27367-59] (ab313378) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a his tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human FKBP12 expression vector containi a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human FKBP12.6 expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 18 kDa

false

Exposure time: 15s