Knockout Tested Rabbit Recombinant Monoclonal FKBP12 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
WB | IP | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
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Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruits SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
FKBP1, FKBP12, FKBP1A, Peptidyl-prolyl cis-trans isomerase FKBP1A, PPIase FKBP1A, 12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, Immunophilin FKBP12, Rotamase, 12 kDa FKBP, FKBP-12, FKBP-1A
Knockout Tested Rabbit Recombinant Monoclonal FKBP12 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR27367-59
Affinity purification Protein A
This antibody recognizes both FKBP12/FKBP1A and FKBP12.6/FKBP1B.
Blue Ice
+4°C
+4°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
FKBP12 also known as FKBP-12 or FKBP12 protein is a small protein with a molecular weight of approximately 12 kDa. It belongs to the family of immunophilins and acts as a peptidyl-prolyl cis-trans isomerase (PPIase) facilitating the folding of proteins by catalyzing the isomerization of proline residues in polypeptides. The FKBP12 protein is expressed in various tissues throughout the body including the brain heart and skeletal muscles. It plays a mechanical role in binding certain macrolide antibiotics such as FK506 (tacrolimus) influencing their biological effects by altering protein conformation.
FKBP12 is significant in cellular mechanisms beyond protein folding. It interacts with and forms complexes with other proteins such as ryanodine receptors and transforming growth factor-beta (TGF-β) receptors impacting calcium signaling and cell growth. FKBP12's interaction with FK506 has been extensively studied because it forms a complex that inhibits calcineurin ultimately leading to immunosuppressive effects. This property of FKBP12 makes it important for modulating the immune response and has importance in transplant medicine.
FKBP12 is actively involved in calcium signaling and TGF-β signaling pathways. FKBP12 influences these pathways by binding to ryanodine receptors affecting calcium release from intracellular stores and also modulating the function of TGF-β receptors impacting cellular growth and proliferation processes. It is associated with proteins such as calcineurin and Smad proteins in these pathways maintaining cellular homeostasis and regulating immune and inflammatory responses.
FKBP12 plays a role in immunological and proliferative diseases. It is implicated in autoimmune disorders due to its interaction with FK506 leading to the suppression of unwanted immune responses. FKBP12 is also linked to cardiac hypertrophy as its modulation of calcium release through ryanodine receptors can affect heart muscle function. The protein interacts with calcineurin in these contexts contributing to pathological processes in these diseases. Researchers target FKBP12 with ELISA and other assays to investigate its involvement in these disorders and assess therapeutic interventions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-FKBP12 antibody [EPR27367-59] ab313378, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody recognizes both FKBP12 and FKBP12.6.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (Anti-FKBP12 antibody [EPR27367-59] ab313378) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containi a his tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human FKBP12 expression vector containi a His-tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human FKBP12.6 expression vector containi a His-tag whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 18 kDa
Exposure time: 15s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody recognizes both FKBP12 and FKBP12.6.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
This data was developed using Anti-FKBP12 antibody [EPR27367-59] ab313378, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.
All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (Anti-FKBP12 antibody [EPR27367-59] ab313378) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) cytoplasmic fraction at 20 µg
Lane 2: SH-SY5Y nuclear fraction at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa
Exposure time: 81s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.
This data was developed using Anti-FKBP12 antibody [EPR27367-59] ab313378, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-FKBP12 antibody [EPR27367-59] ab313378 was shown to bind specifically to FKBP12. A band was observed at 14 kDa in wild-type HAP1 cell lysates whereas loss of signal was observed in the FKBP12 knockout cell line.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (Anti-FKBP12 antibody [EPR27367-59] ab313378) at 1/1000 dilution
Lane 1: Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 20 µg
Lane 2: FKBP12 knockout HAP1 whole cell lysate at 20 µg
Lane 3: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell fresh lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 14 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-FKBP12 antibody [EPR27367-59] ab313378 was shown to bind specifically to FKBP12. A band was observed at 14 kDa in wild-type HAP1 cell lysates whereas loss of signal was observed in the FKBP12 knockout cell line.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
This data was developed using Anti-FKBP12 antibody [EPR27367-59] ab313378, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: A549 (PMID: 27617579).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 81 seconds
All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (Anti-FKBP12 antibody [EPR27367-59] ab313378) at 1/1000 dilution
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 12 kDa
Exposure time: 81s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: A549 (PMID: 27617579).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 81 seconds
This data was developed using Anti-FKBP12 antibody [EPR27367-59] ab313378, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 seconds
All lanes: Western blot - Anti-FKBP12 antibody [EPR27367-59] (Anti-FKBP12 antibody [EPR27367-59] ab313378) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 12 kDa
Exposure time: 48s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 seconds
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