Rabbit Polyclonal FKBP25 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 7 publications.
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Publications
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- Cellular & molecular biology letters 26:502021Esterase D stabilizes FKBP25 to suppress mTORC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuejun Yang,Xinpeng Chen,Wen Yao,Xiaoling Cui,Na Li,ZhaoMin Lin,Baoxiang Zhao,Junying MiaoPubMed 34875997
- Frontiers in cell and developmental biology 9:6258052021FKBP25 Regulates Meiotic Apparatus During Mouse Oocyte Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanni Wang et. al.PubMed 33553183
- Oncology reports :2019FKBP3 mediates oxaliplatin resistance in colorectal cancer cells by regulating HDAC2 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiafeng Tong et. al.PubMed 31524278
- Nature methods 15:909-9122018A high-throughput pipeline for validation of antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrzysztof Sikorski et. al.PubMed 30377371
- Theranostics 7:3078-30892017FKBP3 Promotes Proliferation of Non-Small Cell Lung Cancer Cells through Regulating Sp1/HDAC2/p27.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenzhuo Zhu et. al.PubMed 28839465
- Aging cell 14:265-732015Rapamycin-mediated mTORC2 inhibition is determined by the relative expression of FK506-binding proteins.Applications:WBReactive species:Unspecified reactive speciesKatherine H Schreiber et. al.PubMed 25652038
- FEBS letters 583:621-62009FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| African green monkey | Predicted |
| Cow | Predicted |
| Hamster | Predicted |
| Monkey | Predicted |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.05000-1.00000 µg/mL | Notes - |
Species Mouse | Dilution info 0.05000-1.00000 µg/mL | Notes - |
Species Human | Dilution info 0.05000-1.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Hamster, Cow, Monkey, African green monkey | Dilution info - | Notes - |
Target data
Function
FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
Alternative names
Peptidyl-prolyl cis-trans isomerase FKBP3, PPIase FKBP3, 25 kDa FK506-binding protein, FK506-binding protein 3, Immunophilin FKBP25, Rapamycin-selective 25 kDa immunophilin, Rotamase, 25 kDa FKBP, FKBP-25, FKBP-3, FKBP3, FKBP25
Recommended products
Rabbit Polyclonal FKBP25 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 7 publications.
Alternative names
Peptidyl-prolyl cis-trans isomerase FKBP3, PPIase FKBP3, 25 kDa FK506-binding protein, FK506-binding protein 3, Immunophilin FKBP25, Rapamycin-selective 25 kDa immunophilin, Rotamase, 25 kDa FKBP, FKBP-25, FKBP-3, FKBP3, FKBP25
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Concentration
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FKBP25 also known as FK506 Binding Protein 3 (FKBP3) is a peptidyl-prolyl cis-trans isomerase with a molecular mass of approximately 25 kDa. This protein mainly binds immunosuppressive drugs like tacrolimus and plays an important role in protein folding by accelerating the cis-trans conversion of proline residues in polypeptide chains. FKBP25 is broadly expressed in human tissues with higher expression levels reported in the heart liver and skeletal muscle indicating its significance in various cellular functions.
- Biological function summary
FKBP25 interacts with ribosomal subunits and histones linking it to both ribosome assembly and chromatin regulation processes. It contributes to the formation of complexes involved in maintaining DNA stability and managing gene expression. Through these associations FKBP25 actively participates in cellular signaling and programmed gene transcription therefore significantly affecting cellular proliferation and differentiation mechanisms.
- Pathways
FKBP25 participates in the mTOR signaling pathway impacting cell growth and metabolism. Additionally FKBP25 is involved in the folding and assembly of newly synthesized proteins through its interaction with the Hsp90 chaperone pathway. Its interaction with the mTOR complex as well as with Hsp90 and other co-chaperones highlights its integration into critical cellular survival and stress response mechanisms.
- Associated diseases and disorders
FKBP25 has been linked to certain cancers and neurological diseases. Its interactions with mTOR and Hsp90 relate it to oncogenesis as these pathways are often dysregulated in tumor cells. Additionally alterations in FKBP25 function or expression have been associated with neurodegenerative disorders due to its critical role in protein homeostasis a process related to proteins such as mTOR and Hsp90 involved in cellular stress responses and synaptic signaling.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-FKBP25 antibody (ab16654)
All lanes: Western blot - Anti-FKBP25 antibody (ab16654) at 1 µg/mL
Lanes 1 and 3: HeLa Whole Cell Lysate at 20 µg
Lanes 2 and 4: HeLa Nuclear Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa
This image is courtesy of collaborator dataWestern blot - Anti-FKBP25 antibody (ab16654)
ab16654 specifically recognises FKBP25 (lane1), which can be blocked using the N-terminal immunizing peptide (lane2) but not the corresponding C-terminal peptide (lane3).
All lanes: Western blot - Anti-FKBP25 antibody (ab16654) at 0.05 µg/mL
All lanes: HeLa nuclear extracts at 20 µg
Predicted band size: 25 kDa
Western blot - Anti-FKBP25 antibody (ab16654)
All lanes: Western blot - Anti-FKBP25 antibody (ab16654) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 28 kDa, 58 kDa
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