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AB129113

Anti-FKBP38 antibody [EPR7441(2)]

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(2 Publications)

Rabbit Recombinant Monoclonal FKBP38 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

FKBP38, FKBP8, Peptidyl-prolyl cis-trans isomerase FKBP8, PPIase FKBP8, 38 kDa FK506-binding protein, FK506-binding protein 8, FKBPR38, Rotamase, 38 kDa FKBP, FKBP-38, hFKBP38, FKBP-8

4 Images
Flow Cytometry (Intracellular) - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FKBP38 with ab129113 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)

ab129113, at 1/100 dilution, staining FKBP38 in HeLa cells by Immunofluorescence.

Western blot - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)
  • WB

Unknown

Western blot - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)

All lanes:

Western blot - Anti-FKBP38 antibody [EPR7441(2)] (ab129113) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 45 kDa

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Western blot - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)
  • WB

Lab

Western blot - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : FKBP38 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab129113 observed at 51 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab129113 was shown to specifically react with FKBP38 when FKBP38 knockout samples were used. Wild-type and FKBP38 knockout samples were subjected to SDS-PAGE. ab129113 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FKBP38 antibody [EPR7441(2)] (ab129113)

Predicted band size: 45 kDa

false

  • Carrier free

    Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7441(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FKBP38 also known as FK506 binding protein 8 is a member of the immunophilin protein family characterized by peptidyl-prolyl cis-trans isomerase activity. This protein weighs approximately 42 kDa and plays significant roles in various cellular processes. FKBP38 is mainly expressed in the mitochondria and endoplasmic reticulum where it regulates multiple signaling pathways through its interactions with other complex proteins.
Biological function summary

FKBP38 regulates apoptosis and cell survival mechanisms. It binds to and inhibits the anti-apoptotic proteins Bcl-2 and Bcl-xL forming a complex that influences mitochondrial integrity. This interaction is essential for maintaining cellular homeostasis and responding to stress signals. FKBP38's presence in these complexes highlights its role as an important modulator of cell death pathways contributing to balanced cell proliferation and survival.

Pathways

FKBP38 significantly affects the mTOR signaling pathway and the intrinsic apoptosis pathway. It interacts with important proteins such as Bcl-2 Bcl-xL and mTORC1 influencing cellular growth and apoptosis. FKBP38's regulation of mTOR signaling impacts protein synthesis cell growth and autophagy making it a critical factor in maintaining cellular metabolic balance.

FKBP38 has links to cancer and neurodegenerative diseases. Its modulation of apoptosis and cell proliferation relates to tumor growth and cancer progression where its interaction with Bcl-2 proteins becomes relevant. Additionally FKBP38's role in neurodegenerative diseases ties to its regulation of mitochondrial function where dysregulation leads to neuronal cell death. Understanding FKBP38's connections with these diseases helps in formulating potential therapeutic strategies targeting these important proteins and pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis. Involved in the inhibition of viral infection by influenza A viruses (IAV) (PubMed : 28169297).
See full target information FKBP8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 15:1333-1355 PubMed30767700

2019

Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs.

Applications

Unspecified application

Species

Unspecified reactive species

Åsa Birna Birgisdottir,Stephane Mouilleron,Zambarlal Bhujabal,Martina Wirth,Eva Sjøttem,Gry Evjen,Wenxin Zhang,Rebecca Lee,Nicola O'Reilly,Sharon A Tooze,Trond Lamark,Terje Johansen

EMBO reports 18:947-961 PubMed28381481

2017

FKBP8 recruits LC3A to mediate Parkin-independent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zambarlal Bhujabal,Åsa B Birgisdottir,Eva Sjøttem,Hanne B Brenne,Aud Øvervatn,Sabrina Habisov,Vladimir Kirkin,Trond Lamark,Terje Johansen
View all publications
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