Mouse Monoclonal FKBP51 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Human FKBP5.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [Hi51B] (AB79844), expandable thumbnail](https://content.abcam.com/products/images/fkbp51-antibody-hi51b-ab79844--immunocytochemistry-immunofluorescence-img124334.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [Hi51B] (AB79844)")
![Western blot - Anti-FKBP51 antibody [Hi51B] (AB79844), expandable thumbnail](https://content.abcam.com/products/images/fkbp51-antibody-hi51b-ab79844--western-blot-img100612.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FKBP51 antibody [Hi51B] (AB79844)")
Publications
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- Cell death & disease 14:7362023FKBP5 activates mitophagy by ablating PPAR-γ to shape a benign remyelination environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingzong Sun,Menghan Qian,Hongliang Li,Lei Wang,Yunjie Zhao,Min Yin,Lili Dai,Hongkun BaoPubMed 37952053
- Cell death & disease 14:1162023FKBP51 plays an essential role in Akt ubiquitination that requires Hsp90 and PHLPP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Tufano,Laura Marrone,Chiara D'Ambrosio,Valeria Di Giacomo,Simona Urzini,Yichuan Xiao,Monica Matuozzo,Andrea Scaloni,Maria Fiammetta Romano,Simona RomanoPubMed 36781840
- Experimental eye research 187:1077742019Cellular and cytoskeletal alterations of scleral fibroblasts in response to glucocorticoid steroids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThania Bogarin et. al.PubMed 31449795
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Full Length Protein corresponding to Human FKBP5. The exact immunogen used to generate this antibody is proprietary information. Database link Q13451
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Immunophilin protein with PPIase and co-chaperone activities (PubMed:11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed:12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277, PubMed:28363942). Interacts with IKBKE and IKBKB which facilitates IKK complex assembly leading to increased IKBKE and IKBKB kinase activity, NF-kappa-B activation, and IFN production (PubMed:26101251, PubMed:31434731).
Alternative names
AIG6, FKBP51, FKBP5, Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54
Recommended products
Mouse Monoclonal FKBP51 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Human FKBP5.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Full Length Protein corresponding to Human FKBP5. The exact immunogen used to generate this antibody is proprietary information. Database link Q13451
- Clone number
- Hi51B
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FKBP51 also known as FKBP5 is an immunophilin protein with a molecular mass around 51 kDa. It functions as a peptidyl-prolyl isomerase that assists protein folding by catalyzing the cis-trans isomerization of prolyl bonds. FKBP51 is expressed in various tissues including the brain liver kidney and skeletal muscle. The protein serves multiple mechanical roles including the modulation of protein receptor complexes and interaction with other molecular chaperones.
- Biological function summary
FKBP51 plays a role in cellular stress responses and steroid receptor signaling. It is part of large complexes such as the glucocorticoid receptor complex where it modulates receptor sensitivity and translocation to the nucleus. FKBP51 influences the stress hormone pathway by affecting the receptor's binding affinity and contributing to the regulation of inflammatory responses. By playing a regulatory role in hormonal signaling FKBP51 impacts several biological processes including development and metabolism.
- Pathways
FKBP51 interacts with the hypothalamus-pituitary-adrenal (HPA) axis and the AKT/mTOR signaling pathway. It interacts with proteins like Hsp90 and PHLPP impacting stress response and cellular growth regulation. The modulation of these pathways affects stress-related behaviors and cellular homeostasis. FKBP51's interaction with the HPA axis plays a significant role in stress response regulation making it an essential component of the body's adaptation mechanisms.
- Associated diseases and disorders
FKBP51 has strong associations with mood disorders and cancer. Its upregulation correlates with stress-related psychiatric conditions such as depression impacting the function of other stress-related proteins like glucocorticoid receptors. In cancer FKBP51 modulates tumor growth and survival pathways interacting with proteins involved in cell proliferation and death such as the AKT protein. Understanding FKBP51's role in these conditions helps identify potential therapeutic targets for treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [Hi51B] (ab79844), expandable thumbnail](https://content.abcam.com/products/images/fkbp51-antibody-hi51b-ab79844--immunocytochemistry-immunofluorescence-img124334.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [Hi51B] (ab79844)")
Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [Hi51B] (ab79844)
ab79844 at 1/100 dilution staining FKBP51 in normal mouse MK cells (IF localization shown in red) by Immunocytochemistry/ immunofluorescence.
![Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844), expandable thumbnail](https://content.abcam.com/products/images/fkbp51-antibody-hi51b-ab79844--western-blot-img100612.jpg "Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844)")
Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: HepG2 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab79844 observed at 51 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
ab79844 was shown to recognize FKBP51 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. ab79844 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844)
Predicted band size: 51 kDa
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