AB79844

Anti-FKBP51 antibody [Hi51B]

KO Validated
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(4 Publications)

Mouse Monoclonal FKBP51 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human FKBP5.

View Alternative Names

AIG6, FKBP51, FKBP5, Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54

2 Images

Supplier Data

Western blot - Anti-FKBP51 antibody [Hi51B] (AB79844)

All lanes:

Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844) at 1/1000 dilution

Secondary

All lanes:

Sheep Anti-Mouse IgG: HRP

Predicted band size: 51 kDa

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Lab

Western blot - Anti-FKBP51 antibody [Hi51B] (AB79844)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : FKBP51 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab79844 observed at 51 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab79844 was shown to recognize FKBP51 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. ab79844 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844)

Predicted band size: 51 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Hi51B

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Full Length Protein corresponding to Human FKBP5. The exact immunogen used to generate this antibody is proprietary information.

Q13451

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FKBP51 also known as FKBP5 is an immunophilin protein with a molecular mass around 51 kDa. It functions as a peptidyl-prolyl isomerase that assists protein folding by catalyzing the cis-trans isomerization of prolyl bonds. FKBP51 is expressed in various tissues including the brain liver kidney and skeletal muscle. The protein serves multiple mechanical roles including the modulation of protein receptor complexes and interaction with other molecular chaperones.

Biological function summary

FKBP51 plays a role in cellular stress responses and steroid receptor signaling. It is part of large complexes such as the glucocorticoid receptor complex where it modulates receptor sensitivity and translocation to the nucleus. FKBP51 influences the stress hormone pathway by affecting the receptor's binding affinity and contributing to the regulation of inflammatory responses. By playing a regulatory role in hormonal signaling FKBP51 impacts several biological processes including development and metabolism.

Pathways

FKBP51 interacts with the hypothalamus-pituitary-adrenal (HPA) axis and the AKT/mTOR signaling pathway. It interacts with proteins like Hsp90 and PHLPP impacting stress response and cellular growth regulation. The modulation of these pathways affects stress-related behaviors and cellular homeostasis. FKBP51's interaction with the HPA axis plays a significant role in stress response regulation making it an essential component of the body's adaptation mechanisms.

FKBP51 has strong associations with mood disorders and cancer. Its upregulation correlates with stress-related psychiatric conditions such as depression impacting the function of other stress-related proteins like glucocorticoid receptors. In cancer FKBP51 modulates tumor growth and survival pathways interacting with proteins involved in cell proliferation and death such as the AKT protein. Understanding FKBP51's role in these conditions helps identify potential therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunophilin protein with PPIase and co-chaperone activities (PubMed : 11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed : 12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed : 28147277, PubMed : 28363942). Interacts with IKBKE and IKBKB which facilitates IKK complex assembly leading to increased IKBKE and IKBKB kinase activity, NF-kappa-B activation, and IFN production (PubMed : 26101251, PubMed : 31434731).

See full target information FKBP5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:736 PubMed37952053

2023

FKBP5 activates mitophagy by ablating PPAR-γ to shape a benign remyelination environment.

Applications

Unspecified application

Species

Unspecified reactive species

Xingzong Sun,Menghan Qian,Hongliang Li,Lei Wang,Yunjie Zhao,Min Yin,Lili Dai,Hongkun Bao

Cell death & disease 14:116 PubMed36781840

2023

FKBP51 plays an essential role in Akt ubiquitination that requires Hsp90 and PHLPP.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Tufano,Laura Marrone,Chiara D'Ambrosio,Valeria Di Giacomo,Simona Urzini,Yichuan Xiao,Monica Matuozzo,Andrea Scaloni,Maria Fiammetta Romano,Simona Romano

Experimental eye research 187:107774 PubMed31449795

2019

Cellular and cytoskeletal alterations of scleral fibroblasts in response to glucocorticoid steroids.

Applications

Unspecified application

Species

Unspecified reactive species

Thania Bogarin,Sindhu Saraswathy,Goichi Akiyama,Xiaobin Xie,Robert N Weinreb,Jie Zheng,Alex S Huang

eNeuro 6: PubMed30963102

2019

The Disease-Associated Chaperone FKBP51 Impairs Cognitive Function by Accelerating AMPA Receptor Recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Blair,Marangelie Criado-Marrero,Dali Zheng,Xinming Wang,Siddharth Kamath,Bryce A Nordhues,Edwin J Weeber,Chad A Dickey

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FKBP51 antibody [Hi51B]

Western blot - Anti-FKBP51 antibody [Hi51B] (AB79844)

Western blot - Anti-FKBP51 antibody [Hi51B] (AB79844)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise