AB97306

Anti-FKBP52 antibody

Name: Anti-FKBP52 antibody (ab97306) | Abcam
Brand: Abcam Limited
SKU: AB97306
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal FKBP52 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FKBP4 aa 1-200.

View Alternative Names

FKBP52, FKBP4, Peptidyl-prolyl cis-trans isomerase FKBP4, PPIase FKBP4, 51 kDa FK506-binding protein, 52 kDa FK506-binding protein, 59 kDa immunophilin, FK506-binding protein 4, FKBP59, HSP-binding immunophilin, Immunophilin FKBP52, Rotamase, FKBP51, 52 kDa FKBP, FKBP-52, p59, FKBP-4, HBI

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody (AB97306)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody (AB97306)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab97306 at 1/200 dilution (1) and merged with DNA probe (2).

Supplier Data

Western blot - Anti-FKBP52 antibody (AB97306)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-FKBP52 antibody (ab97306) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Lane 5:

A375 whole cell lysates at 30 µg

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-FKBP52 antibody (AB97306)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-FKBP52 antibody (ab97306) at 1/1000 dilution

Lane 1:

GL261 whole cell lysates at 30 µg

Lane 2:

C8D30 whole cell lysates at 30 µg

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-FKBP52 antibody (AB97306)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-FKBP52 antibody (ab97306) at 1/1000 dilution

All lanes:

Rat2 whole cell lysate at 30 µg

Predicted band size: 52 kDa

false

Unknown

Western blot - Anti-FKBP52 antibody (AB97306)

All lanes:

Western blot - Anti-FKBP52 antibody (ab97306) at 1/3000 dilution

All lanes:

Western blot - Recombinant human FKBP52 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-fkbp52-protein-ab84189'>ab84189</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 52 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-FKBP52 antibody (AB97306)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-FKBP52 antibody (ab97306) at 1/3000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

FKBP4 shRNA transfected (+) 293T whole cell extracts at 30 µg

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FKBP4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q02790

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The immunophilin protein FKBP52 also known as FK506-binding protein 4 (FKBP4) weighs approximately 52 kDa. This protein acts as a peptidyl-prolyl cis-trans isomerase which assists in protein folding and stability. FKBP52 is widely expressed in cells with high levels in tissues like the brain testis and kidneys indicating its role in various cellular processes. As part of its mechanical role FKBP52 interacts with heat shock proteins aiding in the regulation of protein homeostasis.

Biological function summary

FKBP52 functions as a co-chaperone in steroid receptor complexes. It partners with heat shock protein 90 (HSP90) to form a functional complex that modulates the activity of steroid hormone receptors. These receptors such as the androgen and glucocorticoid receptors rely on FKBP52 for proper regulation and transport within the cell. FKBP52 also plays a part in cellular signaling influencing processes like cell growth and differentiation through its interaction with these receptors.

Pathways

FKBP52 features prominently in steroid hormone signaling and stress response pathways. It is critical to the maturation and transport of steroid hormone receptors directly affecting their downstream gene regulation. The protein interacts closely with HSP90 and other immunophilins like FKBP51 which also plays roles in similar pathways but may have differing regulatory effects. This interaction demonstrates how FKBP52's function integrates into broader cellular signaling networks influencing pathways like the glucocorticoid receptor signaling pathway.

FKBP52 connects significantly to prostate cancer and Alzheimer's disease. In prostate cancer its modulation of androgen receptor activity is critical. Abnormal regulation of these receptors can contribute to cancer progression. Moreover in Alzheimer's disease FKBP52 influences the stability and function of tau protein which is linked to neurofibrillary tangle formation a hallmark of the disease. Its interaction with tau indicates a potential therapeutic target for intervention in Alzheimer's progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.

See full target information FKBP4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Research in veterinary science 152:510-523 PubMed36174371

2022

NR3C1 and glucocorticoid-regulatory genes mRNA and protein expression in the endometrium and ampulla during the bovine estrous cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Mateo Ruiz-Conca,Jaume Gardela,Sergi Olvera-Maneu,Manel López-Béjar,Manuel Álvarez-Rodríguez

Organogenesis 17:1-13 PubMed33464989

2021

The Influence Mechanism of Abnormal Immunophilin FKBP52 on the Expression Levels of PR-A and PR-B in Endometriosis Based on Endometrial Stromal Cell Model in Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Liling Liu,Junping Cheng,Fu Wei,Lihong Pang,Zhifu Zhi,Wenmei Yang,Weihong Tan

Journal of animal science and biotechnology 7:31 PubMed27231548

2016

Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Xiao Chen,Jinluan Fu,Aiguo Wang

Molecular biology of the cell 24:2303-10 PubMed23741051

2013

Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Justin M Pare,Paul LaPointe,Tom C Hobman

Journal of proteomics 75:4457-70 PubMed22507201

2012

Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection.

Applications

Species

Pig

Rodrigo Prado Martins,Melania Collado-Romero,Montserrat Martínez-Gomáriz,Ana Carvajal,Concepción Gil,Concepción Lucena,Angela Moreno,Juan J Garrido

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FKBP52 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody (AB97306)

Western blot - Anti-FKBP52 antibody (AB97306)

Western blot - Anti-FKBP52 antibody (AB97306)

Western blot - Anti-FKBP52 antibody (AB97306)

Western blot - Anti-FKBP52 antibody (AB97306)

Western blot - Anti-FKBP52 antibody (AB97306)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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