AB230951

Anti-FKBP52 antibody [EPR21125]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal FKBP52 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

FKBP52, FKBP4, Peptidyl-prolyl cis-trans isomerase FKBP4, PPIase FKBP4, 51 kDa FK506-binding protein, 52 kDa FK506-binding protein, 59 kDa immunophilin, FK506-binding protein 4, FKBP59, HSP-binding immunophilin, Immunophilin FKBP52, Rotamase, FKBP51, 52 kDa FKBP, FKBP-52, p59, FKBP-4, HBI

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (AB230951)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (AB230951)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling FKBP52 with ab230951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line.

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of the primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-FKBP52 antibody [EPR21125] (AB230951)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-FKBP52 antibody [EPR21125] (AB230951)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling FKBP52 with ab230951 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^ï¿½ 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (AB230951)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling FKBP52 with ab230951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in MCF7 cell line.

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of the primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Unknown

Immunoprecipitation - Anti-FKBP52 antibody [EPR21125] (AB230951)

FKBP52 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab230951 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab230951 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230951 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-FKBP52 antibody [EPR21125] (ab230951)

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Unknown

Western blot - Anti-FKBP52 antibody [EPR21125] (AB230951)

Blocking/Diluting buffer : 5% NFDM/TBST.

Exposure times : Lane 1-2 : 48 seconds; Lane 3 : 30 seconds; Lane 4 : 3 minutes.

The molecular mass observed is consistent with what has been described in the literature (PMID : 26065228).

Lanes 1 - 2:

Western blot - Anti-FKBP52 antibody [EPR21125] (ab230951) at 1/5000 dilution

Lanes 3 - 4:

Western blot - Anti-FKBP52 antibody [EPR21125] (ab230951) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 4:

Human fetal brain lysate at 10 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 4:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Carrier free

Anti-FKBP52 antibody [EPR21125] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21125

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The immunophilin protein FKBP52 also known as FK506-binding protein 4 (FKBP4) weighs approximately 52 kDa. This protein acts as a peptidyl-prolyl cis-trans isomerase which assists in protein folding and stability. FKBP52 is widely expressed in cells with high levels in tissues like the brain testis and kidneys indicating its role in various cellular processes. As part of its mechanical role FKBP52 interacts with heat shock proteins aiding in the regulation of protein homeostasis.

Biological function summary

FKBP52 functions as a co-chaperone in steroid receptor complexes. It partners with heat shock protein 90 (HSP90) to form a functional complex that modulates the activity of steroid hormone receptors. These receptors such as the androgen and glucocorticoid receptors rely on FKBP52 for proper regulation and transport within the cell. FKBP52 also plays a part in cellular signaling influencing processes like cell growth and differentiation through its interaction with these receptors.

Pathways

FKBP52 features prominently in steroid hormone signaling and stress response pathways. It is critical to the maturation and transport of steroid hormone receptors directly affecting their downstream gene regulation. The protein interacts closely with HSP90 and other immunophilins like FKBP51 which also plays roles in similar pathways but may have differing regulatory effects. This interaction demonstrates how FKBP52's function integrates into broader cellular signaling networks influencing pathways like the glucocorticoid receptor signaling pathway.

FKBP52 connects significantly to prostate cancer and Alzheimer's disease. In prostate cancer its modulation of androgen receptor activity is critical. Abnormal regulation of these receptors can contribute to cancer progression. Moreover in Alzheimer's disease FKBP52 influences the stability and function of tau protein which is linked to neurofibrillary tangle formation a hallmark of the disease. Its interaction with tau indicates a potential therapeutic target for intervention in Alzheimer's progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.

See full target information FKBP4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40565313

2025

Immune-Checkpoint Expression in Breast Cancer Patients: Clinicopathological Implications: A Retrospective Case Series Study.

Applications

Unspecified application

Species

Unspecified reactive species

Angel Quiroz-Bolaños,Antonio Quintero-Ramos,Juliana Marisol Godínez-Rubí,Ramon Franco-Topete,Porfirio Gutiérrez González,Bricia M Gutiérrez-Zepeda,Denisse S Becerra-Loaiza,Antonio Topete,Cesar de Loera-Rodriguez,Alicia Del Toro-Arreola,Adrián Daneri-Navarro

Cell death & disease 12:602 PubMed34112753

2021

FKBP4 integrates FKBP4/Hsp90/IKK with FKBP4/Hsp70/RelA complex to promote lung adenocarcinoma progression via IKK/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zong,Yulian Jiao,Xin Liu,Wenli Mu,Xiaotian Yuan,Yunyun Qu,Yu Xia,Shuang Liu,Huanxin Sun,Laicheng Wang,Bin Cui,Xiaowen Liu,Ping Li,Yueran Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FKBP52 antibody [EPR21125]

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (AB230951)

Flow Cytometry (Intracellular) - Anti-FKBP52 antibody [EPR21125] (AB230951)

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR21125] (AB230951)

Immunoprecipitation - Anti-FKBP52 antibody [EPR21125] (AB230951)

Western blot - Anti-FKBP52 antibody [EPR21125] (AB230951)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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