AB129097

Anti-FKBP52 antibody [EPR6618]

RabMAb
Recombinant
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(13 Publications)

Rabbit Recombinant Monoclonal FKBP52 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

View Alternative Names

FKBP52, FKBP4, Peptidyl-prolyl cis-trans isomerase FKBP4, PPIase FKBP4, 51 kDa FK506-binding protein, 52 kDa FK506-binding protein, 59 kDa immunophilin, FK506-binding protein 4, FKBP59, HSP-binding immunophilin, Immunophilin FKBP52, Rotamase, FKBP51, 52 kDa FKBP, FKBP-52, p59, FKBP-4, HBI

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (AB129097)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (AB129097)

ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

DAPI was used as a nuclear counterstain and the negative control was PBS only.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (AB129097)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (AB129097)

ab129097 at a 1/250 dilution staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (AB129097)

ab129097 at a 1/250 dilution staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-FKBP52 antibody [EPR6618] (AB129097)

All lanes:

Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 10 µg

Lane 2:

Jurkat cell lysates at 10 µg

Lane 3:

K562 cell lysates at 10 µg

Lane 4:

MCF7 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

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OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (AB129097)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (AB129097)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

CiteAb

Western blot - Anti-FKBP52 antibody [EPR6618] (AB129097)

FKBP52 western blot using anti-FKBP52 antibody [EPR6618] ab129097. Publication image and figure legend from Xiao, K., Yang, L., et al., 2020, Biomed Res Int, PubMed 32908868.

ab129097 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab129097 please see the product overview.

Effect of TNF-α on inflammatory response and apoptosis of LCLs and B lymphocytes. (a) Protein expression of TRAF1, IKK-α, NIK, and p52 was measured by western blot. (b) Flow cytometry analysis of apoptosis. (c) Protein expression of pro-Caspase 3, caspase 3, and PARP was measured by western blot. The results are presented as the mean ± SD, n = 3. *p < 0.05 vs. control.

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6618

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The immunophilin protein FKBP52 also known as FK506-binding protein 4 (FKBP4) weighs approximately 52 kDa. This protein acts as a peptidyl-prolyl cis-trans isomerase which assists in protein folding and stability. FKBP52 is widely expressed in cells with high levels in tissues like the brain testis and kidneys indicating its role in various cellular processes. As part of its mechanical role FKBP52 interacts with heat shock proteins aiding in the regulation of protein homeostasis.

Biological function summary

FKBP52 functions as a co-chaperone in steroid receptor complexes. It partners with heat shock protein 90 (HSP90) to form a functional complex that modulates the activity of steroid hormone receptors. These receptors such as the androgen and glucocorticoid receptors rely on FKBP52 for proper regulation and transport within the cell. FKBP52 also plays a part in cellular signaling influencing processes like cell growth and differentiation through its interaction with these receptors.

Pathways

FKBP52 features prominently in steroid hormone signaling and stress response pathways. It is critical to the maturation and transport of steroid hormone receptors directly affecting their downstream gene regulation. The protein interacts closely with HSP90 and other immunophilins like FKBP51 which also plays roles in similar pathways but may have differing regulatory effects. This interaction demonstrates how FKBP52's function integrates into broader cellular signaling networks influencing pathways like the glucocorticoid receptor signaling pathway.

FKBP52 connects significantly to prostate cancer and Alzheimer's disease. In prostate cancer its modulation of androgen receptor activity is critical. Abnormal regulation of these receptors can contribute to cancer progression. Moreover in Alzheimer's disease FKBP52 influences the stability and function of tau protein which is linked to neurofibrillary tangle formation a hallmark of the disease. Its interaction with tau indicates a potential therapeutic target for intervention in Alzheimer's progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.

See full target information FKBP4

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:31037 PubMed40849525

2025

Proteomics on choroidal neovascularization based on itraq and the protective effect of TAB1 in CNV.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Du,Shaoqiu Jiang,Lujia Feng,Jing Lu,Hui Peng,Xiyuan Zhou

Cell chemical biology 31:1162-1175.e10 PubMed38320555

2024

The cyclimids: Degron-inspired cereblon binders for targeted protein degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Saki Ichikawa,N Connor Payne,Wenqing Xu,Chia-Fu Chang,Nandini Vallavoju,Spencer Frome,Hope A Flaxman,Ralph Mazitschek,Christina M Woo

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 72:9-17 PubMed37040689

2023

Kappa opioid activation changes protein profiles in different regions of the brain relevant to depression.

Applications

Unspecified application

Species

Unspecified reactive species

Bardia Varastehmoradi,Karen L Smith,Heidi Kaastrup Müller,Betina Elfving,Connie Sanchez,Gregers Wegener

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 52:389-401 PubMed36153671

2022

Development and validation of a gene model predicting lymph node metastasis and prognosis of oral squamous cell carcinoma based on single-cell and bulk RNA-seq analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Zhen Han,Li-Cheng Tan,Qing-Si Ouyang,Peng-Cheng Yu,Xiao Shi,Jia-Qian Hu,Wen-Jun Wei,Zhong-Wu Lu,Yu Wang,Qing-Hai Ji,Ning Qu,Hua-Ming Mai,Yu-Long Wang

International journal of molecular sciences 23: PubMed35628305

2022

Effects of RU486 in Treatment of Traumatic Stress-Induced Glucocorticoid Dysregulation and Fear-Related Abnormalities: Early versus Late Intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Cheng Lin,Pao-Yun Cheng,Michael Hsiao,Yia-Ping Liu

Annals of translational medicine 9:1624 PubMed34926668

2021

Hyperglycemia-induced increasing of /circ_0008590 in NF-κB pathway is repressed by miR-1243 in human retinal microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Shao,Jiping Cai,Yong Yao,Hui Zhu

Cell death & disease 12:602 PubMed34112753

2021

FKBP4 integrates FKBP4/Hsp90/IKK with FKBP4/Hsp70/RelA complex to promote lung adenocarcinoma progression via IKK/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zong,Yulian Jiao,Xin Liu,Wenli Mu,Xiaotian Yuan,Yunyun Qu,Yu Xia,Shuang Liu,Huanxin Sun,Laicheng Wang,Bin Cui,Xiaowen Liu,Ping Li,Yueran Zhao

BioMed research international 2020:1481572 PubMed32908868

2020

HuR Affects Proliferation and Apoptosis of Chronic Lymphocytic Leukemia Cells via NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Xiao,Lin Yang,Xinfeng Gao,Ying An,Wei Xie,Guo Jingquan

Behavioural brain research 391:112686 PubMed32428628

2020

Effects of early life social experience on fear extinction and related glucocorticoid profiles - behavioral and neurochemical approaches in a rat model of PTSD.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Cheng Lin,Pao-Yun Cheng,Yia-Ping Liu

International journal of molecular medicine 43:868-878 PubMed30483787

2018

Expression of FKBP52 in the ovaries of PCOS rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyan Song,Yong Tan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FKBP52 antibody [EPR6618]

Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (AB129097)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (AB129097)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (AB129097)

Western blot - Anti-FKBP52 antibody [EPR6618] (AB129097)

OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (AB129097)

Western blot - Anti-FKBP52 antibody [EPR6618] (AB129097)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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