AB279389

Anti-FKLF / KLF11 antibody [OTI4H7]

Name: Anti-FKLF / KLF11 antibody [OTI4H7] (ab279389) | Abcam
Brand: Abcam Limited
SKU: AB279389
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal FKLF / KLF11 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLF11 aa 1-300.

View Alternative Names

FKLF, TIEG2, KLF11, Krueppel-like factor 11, Transforming growth factor-beta-inducible early growth response protein 2, TGFB-inducible early growth response protein 2, TIEG-2

2 Images

Supplier Data

Western blot - Anti-FKLF / KLF11 antibody [OTI4H7] (AB279389)

All lanes:

Western blot - Anti-FKLF / KLF11 antibody [OTI4H7] (ab279389) at 1/500 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell extract at 35 µg

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-FKLF / KLF11 antibody [OTI4H7] (AB279389)

All lanes:

Western blot - Anti-FKLF / KLF11 antibody [OTI4H7] (ab279389) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with the pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells pCMV6-ENTRY FKLF / KLF11 cDNA for 48 hrs and lysed at 5 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4H7

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human KLF11 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O14901

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FKLF also known as KLF11 is a transcription factor of the Krüppel-like family vital for regulating gene expression. This protein has a mass of approximately 55 kDa and is expressed widely in various human tissues including the pancreas liver and heart. KLF11 has functional domains that bind to specific DNA sequences influencing the transcription process. The protein can act as an activator or repressor impacting cellular responses depending on the context of DNA.

Biological function summary

The transcription factor KLF11 plays significant roles in cellular differentiation and proliferation. It serves as part of a regulatory complex involved in modulating gene expression associated with cell cycle control and apoptosis. KLF11 exerts its effects by binding to specific promoter regions in target genes facilitating transcriptional activation or repression to modulate cellular states. The protein's activity is important for maintaining normal cell function and tissue homeostasis.

Pathways

KLF11 is integrated into important signaling networks such as the TGF-beta and SMAD pathways. In these pathways KLF11 interacts with various proteins including SMAD3 and SMAD4 to regulate downstream transcriptional responses. By influencing these pathways KLF11 participates in controlling processes like cell growth inhibition and apoptosis ensuring regulated cell proliferation and differentiation. These interactions highlight KLF11's role in orchestrating multiple cellular functions and maintaining physiological balance.

Mutations or dysregulation of KLF11 expression have been implicated in diabetes mellitus and certain cancers. In diabetes KLF11’s modulation of insulin gene expression links it to metabolic regulation where it affects glucose homeostasis. In some cases of cancer aberrant KLF11 activity contributes to uncontrolled cell proliferation suggesting a tumor suppressor role. KLF11 associates with target proteins like SMAD3 during these disease processes highlighting its involvement in their pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor (PubMed : 10207080, PubMed : 9748269). Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth (PubMed : 10207080, PubMed : 16131492, PubMed : 9748269). Represses transcription of SMAD7 which enhances TGF-beta signaling (By similarity). Induces apoptosis (By similarity).

See full target information KLF11

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5392 PubMed40562785

2025

Obesity drives depot-specific vascular remodeling in male white adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Sana S Hasan,David John,Martina Rudnicki,Ibrahim AlZaim,Daniel Eberhard,Iris Moll,Jacqueline Taylor,Christian Klein,Maximilian von Heesen,Lena-Christin Conradi,Ralf H Adams,Eckhard Lammert,Joanna Kalucka,Christiana Ruhrberg,Stefanie Dimmeler,Andreas Fischer

Cell biology international 46:1144-1155 PubMed35293659

2022

KLF11 promotes the progression of glioma via regulating Holliday junction recognition protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Chunhong Wang,Rui Cheng,Haiyang Su,Lijun Wang,Lei Ji,Hongming Ji

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FKLF / KLF11 antibody [OTI4H7]

Western blot - Anti-FKLF / KLF11 antibody [OTI4H7] (AB279389)

Western blot - Anti-FKLF / KLF11 antibody [OTI4H7] (AB279389)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise