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AB236739

Anti-FKSG14/p33 antibody

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(2 Publications)

Rabbit Polyclonal FKSG14/p33 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CENPK.

View Alternative Names

ICEN37, FKSG14, CENPK, Centromere protein K, CENP-K, Interphase centromere complex protein 37, Protein AF-5alpha, p33

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKSG14/p33 antibody (AB236739)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKSG14/p33 antibody (AB236739)

Paraffin-embedded human liver tissue stained for FKSG14/p33 using ab236739 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKSG14/p33 antibody (AB236739)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKSG14/p33 antibody (AB236739)

Paraffin-embedded human skin tissue stained for FKSG14/p33 using ab236739 at 1/100 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-FKSG14/p33 antibody (AB236739)
  • IP

Supplier Data

Immunoprecipitation - Anti-FKSG14/p33 antibody (AB236739)

Immunoprecipitating FKSG14/p33 in Hela whole cell lysate
Lane 1 : Rabbit control IgG instead of ab236739 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2 : ab236739 (8μg) + Hela whole cell lysate (500μg)
Lane 3 : HeLa whole cell lysate (10μg)

All lanes:

Immunoprecipitation - Anti-FKSG14/p33 antibody (ab236739)

Predicted band size: 32 kDa

false

Western blot - Anti-FKSG14/p33 antibody (AB236739)
  • WB

Supplier Data

Western blot - Anti-FKSG14/p33 antibody (AB236739)

All lanes:

Western blot - Anti-FKSG14/p33 antibody (ab236739) at 1/1000 dilution

Lane 1:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CENPK.

Q9BS16

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FKSG14/p33 protein also known as Fuzzy Onions Homolog 2 or FUZ is a component involved in cellular processes like protein trafficking and vesicle transport. FKSG14 has a molecular weight of approximately 33 kDa. You often find it expressed in various tissues including neuronal and epithelial. Functionally FKSG14 plays a role in maintaining the dynamic organization of the cell through its involvement in cytoplasmic transport routes.
Biological function summary

FKSG14 participates in pathways linked to ciliogenesis and cellular asymmetry. It operates as part of the intraflagellar transport (IFT) complex which is essential in the development of primary cilia. These cilia play key roles in cell signaling and development. Presence of FKSG14 within the IFT complex underlines its importance to processes like organ lateralization and embryonic patterning. Its lack or dysfunction can disrupt normal signaling pathways critical for cellular and developmental functions.

Pathways

FKSG14 influences both the Hedgehog and Wnt signaling pathways. These pathways are important for cell differentiation and cell fate determination. Within these pathways FKSG14 interacts with proteins like Smo (Smoothened) in the Hedgehog pathway and Dvl (Disheveled) in the Wnt pathway. These relationships highlight FKSG14's involvement in complex signaling networks illustrating its key position in maintaining cellular integrity and function.

FKSG14 has links to conditions like ciliopathies and neural tube defects. Ciliopathies are disorders resulting from defects in cilia structure or function and can include polycystic kidney disease and Bardet-Biedl syndrome. FKSG14's disruption may connect with these disorders through its interaction with other ciliary proteins like IFT74. It also plays roles in neural development suggesting its involvement in neural tube defects when FKSG14 or related proteins like SHH (Sonic Hedgehog) pathways are impaired leading to congenital anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
See full target information CENPK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:691-699 PubMed34118147

2021

Upregulation of centromere protein K is crucial for lung adenocarcinoma cell viability and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Yongbo Wang,Yue Wang,Chunna Ren,Haicun Wang,Yang Zhang,Yunxia Xiu

Journal of cellular and molecular medicine : PubMed33085209

2020

Integrative analysis provides multi-omics evidence for the pathogenesis of placenta percreta.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyuan Jiang,Lei Dai,Na Chen,Junshu Li,Yan Gao,Jing Zhao,Li Ding,Chengbin Xie,Xiaolian Yi,Hongxin Deng,Xiaodong Wang
View all publications
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Product promise

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