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AB106146

Anti-Flagellin antibody

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(1 Publication)

Rabbit Polyclonal FLA1 antibody. Suitable for WB, ELISA and reacts with Recombinant full length protein - Borreliella burgdorferi B31, Borreliella burgdorferi B31 samples. Cited in 1 publication.

View Alternative Names

BB_0147, fla, Flagellar filament 41 kDa core protein, Flagellin, 41 kDa antigen, P41

1 Images
Western blot - Anti-Flagellin antibody (AB106146)
  • WB

Unknown

Western blot - Anti-Flagellin antibody (AB106146)

All lanes:

Western blot - Anti-Flagellin antibody (ab106146) at 1/1000 dilution

Lane 1:

MBP-Flagellin fusion protein (the immunogen) at 0.1 µg

Lane 2:

MBP alone at 0.1 µg

Secondary

All lanes:

Goat anti-rabbit IgG at 1/40000 dilution

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

ELISA, WB

applications

Specificity

This antibody is specific for Lyme Borrelia spp. Flagellin protein. A BLAST analysis was used to suggest cross-reactivity with Flagellin from B. burgdorferi, garinii, and valaisiana sources based on 100% homology with the immunizing sequence.. Cross-reactivity with Flagellin from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Borreliella burgdorferi B31": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/6000", "ELISA-species-notes": "<p></p>" }, "Recombinant full length protein - Borreliella burgdorferi B31": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water or aqueous buffer at 0.1mL
Purification technique
Affinity purification Protein A
Purification notes
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flagellin is a structural protein essential for the formation and function of bacterial flagella which is critical for motility. Also known as flagellin P41 this protein typically measures around 41 kDa in molecular mass. It plays an important role in the self-assembly of the flagellum's filament creating a helical structure. Expressed in motile bacterial species flagellin forms the building blocks that contribute to the rotation and movement of the flagellum allowing bacteria to navigate their environments.
Biological function summary

Flagellin proteins initiate immune responses as a pathogen-associated molecular pattern (PAMP). It forms part of the complex bacterial flagellar filament. When exposed to host organisms such as humans it can trigger innate immune responses via Toll-like receptor 5 (TLR5). This interaction stimulates cytokine production which can induce an immediate immune system reply to pathogenic bacterial invasion. Due to these interactions flagellin plays an essential role in bridging pathogen encounter and host defense mechanisms.

Pathways

Flagellin interacts significantly with the immune signaling system notably the NF-kB signaling pathway. Upon flagellin binding to TLR5 it activates a cascade leading to NF-kB pathway activation. This results in the transcription of various genes involved in inflammation and immune responses. Flagellin also links indirectly to the MAPK signaling pathway through TLR5 which mediates inflammatory responses. These pathways highlight flagellin's pivotal role in immune system activation and regulation.

Flagellin relates to inflammatory conditions such as inflammatory bowel disease (IBD) and sepsis. In IBD aberrant immune response to E. coli flagellin coupled with interactions with bacterial proteins can exacerbate intestinal inflammation. Sepsis a severe systemic inflammatory condition might involve excessive or uncontrolled response to flagellin leading to harmful immune activation. Understanding these relationships provides valuable insights into flagellin's role in pathological immune activation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the core of the flagella.
See full target information fla
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunological methods 133:181-90 PubMed2146321

1990

Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays.

Applications

Unspecified application

Species

Unspecified reactive species

M Oppermann,H Baumgarten,E Brandt,W Gottsleben,C Kurts,O Götze
View all publications
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Product promise

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