AB93713

Anti-Flagellin antibody

Name: Anti-Flagellin antibody (ab93713) | Abcam
Brand: Abcam Limited
SKU: AB93713
Price: 400 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(36 Publications)

Anti-Flagellin antibody (ab93713) is a rabbit polyclonal antibody detecting Flagellin in Western Blot, ICC/IF. Suitable for Escherichia coli, Salmonella, Salmonella anatum, Salmonella selandia.

- Over 30 publications
- Trusted since 2010

View Alternative Names

flaF, hag, b1923, JW1908, fliC, Flagellin

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Flagellin antibody (AB93713)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flagellin antibody (AB93713)

ab93713 (1 : 100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.

Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flagellin antibody (AB93713)

Unknown

Western blot - Anti-Flagellin antibody (AB93713)

All lanes:

Western blot - Anti-Flagellin antibody (ab93713) at 1/10000 dilution

All lanes:

293T transfected with recombinant protein

Predicted band size: 51 kDa

Observed band size: 60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Escherichia coli, Salmonella, Salmonella anatum, Salmonella selandia

Applications

WB, ICC/IF

applications

Specificity

This antibody is specific for bacterial Flagellin (FliC).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Recombinant full length protein - Salmonella": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Salmonella": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Salmonella anatum": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Salmonella selandia": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Flagellin antibody (ab93713) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Escherichia coli, Salmonella, Salmonella anatum, Salmonella selandia samples.

Trusted by the scientific community
Anti-Flagellin (ab93713) was first used in a scientific publication in 2010 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flagellin is a structural protein essential for the formation and function of bacterial flagella which is critical for motility. Also known as flagellin P41 this protein typically measures around 41 kDa in molecular mass. It plays an important role in the self-assembly of the flagellum's filament creating a helical structure. Expressed in motile bacterial species flagellin forms the building blocks that contribute to the rotation and movement of the flagellum allowing bacteria to navigate their environments.

Biological function summary

Flagellin proteins initiate immune responses as a pathogen-associated molecular pattern (PAMP). It forms part of the complex bacterial flagellar filament. When exposed to host organisms such as humans it can trigger innate immune responses via Toll-like receptor 5 (TLR5). This interaction stimulates cytokine production which can induce an immediate immune system reply to pathogenic bacterial invasion. Due to these interactions flagellin plays an essential role in bridging pathogen encounter and host defense mechanisms.

Pathways

Flagellin interacts significantly with the immune signaling system notably the NF-kB signaling pathway. Upon flagellin binding to TLR5 it activates a cascade leading to NF-kB pathway activation. This results in the transcription of various genes involved in inflammation and immune responses. Flagellin also links indirectly to the MAPK signaling pathway through TLR5 which mediates inflammatory responses. These pathways highlight flagellin's pivotal role in immune system activation and regulation.

Flagellin relates to inflammatory conditions such as inflammatory bowel disease (IBD) and sepsis. In IBD aberrant immune response to E. coli flagellin coupled with interactions with bacterial proteins can exacerbate intestinal inflammation. Sepsis a severe systemic inflammatory condition might involve excessive or uncontrolled response to flagellin leading to harmful immune activation. Understanding these relationships provides valuable insights into flagellin's role in pathological immune activation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.

See full target information fliC

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 17:2489768 PubMed40243695

2025

Exploring the mechanism of intestinal bacterial translocation after severe acute pancreatitis: the role of Toll-like receptor 5.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Zhang,Shiyin Chen,Zhien Wang,Jian Zhang,Wenqiao Yu,Yanshuai Wang,Weiwei Si,Yuwei Zhang,Yun Zhang,Tingbo Liang

Frontiers in microbiology 16:1507286 PubMed39973929

2025

Biochemical characterization of the surfaceome: a focus on type I fimbriae and flagella.

Applications

Unspecified application

Species

Unspecified reactive species

Devon W Kavanaugh,Adeline Sivignon,Yannick Rossez,Zina Chouit,Christophe Chambon,Louane Béal,Mathilde Bonnet,Michel Hébraud,Yann Guérardel,Hang Thi Thu Nguyen,Nicolas Barnich

Communications biology 8:228 PubMed39948151

2025

The proteomic landscape of stool-derived extracellular vesicles in patients with pre-cancerous lesions and colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emmalee J Northrop-Albrecht,Yohan Kim,William R Taylor,Shounak Majumder,John B Kisiel,Fabrice Lucien

Scientific reports 15:4507 PubMed39915561

2025

Purpurin suppresses Salmonella invasion of host cells by reducing the secretion of T3SS-1 effector proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxu Shi,Zhimin Guo,Siqi Li,Chenxiao Jiang,Jianfeng Wang,Xuming Deng,Hongtao Liu,Jiazhang Qiu

eLife 12: PubMed37843988

2023

σ-dependent small RNA regulation of flagella biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sahar Melamed,Aixia Zhang,Michal Jarnik,Joshua Mills,Aviezer Silverman,Hongen Zhang,Gisela Storz

Biotechnology and bioengineering 120:3368-3380 PubMed37555379

2023

High performance anion exchange chromatography purification of probiotic bacterial extracellular vesicles enhances purity and anti-inflammatory efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas H Pirolli,Laura Samantha C Reus,Zuzanna Mamczarz,Sulayman Khan,William E Bentley,Steven M Jay

Frontiers in microbiology 13:1093670 PubMed36619993

2022

Motility increase of adherent invasive (AIEC) induced by a sub-inhibitory concentration of recombinant endolysin LysPA90.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Jung Hwang,Jaehak Jo,Eunsuk Kim,Hyunjin Yoon,Hyewon Hong,Min Soo Kim,Heejoon Myung

Science advances 8:eade8971 PubMed36542710

2022

Interference of flagellar rotation up-regulates the expression of small RNA contributing to infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yukihiro Hiramatsu,Takashi Nishida,Dendi Krisna Nugraha,Mayuko Osada-Oka,Daisuke Nakane,Katsumi Imada,Yasuhiko Horiguchi

International journal of molecular sciences 23: PubMed35955615

2022

Regulator STM0347 Mediates Flagellar Phase Variation via Hin Invertase.

Applications

Unspecified application

Species

Unspecified reactive species

Hongou Wang,Zhiheng Tang,Baoshuai Xue,Qinghui Lu,Xiaoyun Liu,Qinghua Zou

NPJ biofilms and microbiomes 8:39 PubMed35546554

2022

Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyang Li,Lianying Cao,Heike Bähre,Soo-Kyoung Kim,Kristen Schroeder,Kristina Jonas,Kira Koonce,Solomon A Mekonnen,Soumitra Mohanty,Fengwu Bai,Annelie Brauner,Vincent T Lee,Manfred Rohde,Ute Römling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Flagellin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Flagellin antibody (AB93713)

Immunocytochemistry/ Immunofluorescence - Anti-Flagellin antibody (AB93713)

Western blot - Anti-Flagellin antibody (AB93713)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

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