Rabbit Recombinant Monoclonal Flagellin antibody. Carrier free. Suitable for WB and reacts with Escherichia coli, Transfected cell lysate - Escherichia coli K-12 samples.
Images
![Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (AB316011), expandable thumbnail](https://content.abcam.com/products/images/flagellin-antibody-epr28356-51-bsa-and-azide-free-ab316011--western-blot-img426205.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (AB316011)")
![Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (AB316011), expandable thumbnail](https://content.abcam.com/products/images/flagellin-antibody-epr28356-51-bsa-and-azide-free-ab316011--western-blot-img426206.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (AB316011)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | IP | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Escherichia coli | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Escherichia coli K-12 | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli, Transfected cell lysate - Escherichia coli K-12 | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli, Transfected cell lysate - Escherichia coli K-12 | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli, Transfected cell lysate - Escherichia coli K-12 | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli, Transfected cell lysate - Escherichia coli K-12 | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli, Transfected cell lysate - Escherichia coli K-12 | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
Alternative names
flaF, hag, b1923, JW1908, fliC, Flagellin
Recommended products
Rabbit Recombinant Monoclonal Flagellin antibody. Carrier free. Suitable for WB and reacts with Escherichia coli, Transfected cell lysate - Escherichia coli K-12 samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28356-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316011 is the carrier-free version of Anti-Flagellin antibody [EPR28356-51] ab316010.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flagellin is a structural protein essential for the formation and function of bacterial flagella which is critical for motility. Also known as flagellin P41 this protein typically measures around 41 kDa in molecular mass. It plays an important role in the self-assembly of the flagellum's filament creating a helical structure. Expressed in motile bacterial species flagellin forms the building blocks that contribute to the rotation and movement of the flagellum allowing bacteria to navigate their environments.
- Biological function summary
Flagellin proteins initiate immune responses as a pathogen-associated molecular pattern (PAMP). It forms part of the complex bacterial flagellar filament. When exposed to host organisms such as humans it can trigger innate immune responses via Toll-like receptor 5 (TLR5). This interaction stimulates cytokine production which can induce an immediate immune system reply to pathogenic bacterial invasion. Due to these interactions flagellin plays an essential role in bridging pathogen encounter and host defense mechanisms.
- Pathways
Flagellin interacts significantly with the immune signaling system notably the NF-kB signaling pathway. Upon flagellin binding to TLR5 it activates a cascade leading to NF-kB pathway activation. This results in the transcription of various genes involved in inflammation and immune responses. Flagellin also links indirectly to the MAPK signaling pathway through TLR5 which mediates inflammatory responses. These pathways highlight flagellin's pivotal role in immune system activation and regulation.
- Associated diseases and disorders
Flagellin relates to inflammatory conditions such as inflammatory bowel disease (IBD) and sepsis. In IBD aberrant immune response to E. coli flagellin coupled with interactions with bacterial proteins can exacerbate intestinal inflammation. Sepsis a severe systemic inflammatory condition might involve excessive or uncontrolled response to flagellin leading to harmful immune activation. Understanding these relationships provides valuable insights into flagellin's role in pathological immune activation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (ab316011), expandable thumbnail](https://content.abcam.com/products/images/flagellin-antibody-epr28356-51-bsa-and-azide-free-ab316011--western-blot-img426205.jpg "Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (ab316011)")
Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (ab316011)
Flagellin Western blot staining using rabbit Anti-Flagellin antibody
This data was developed using Anti-Flagellin antibody [EPR28356-51] ab316010, the same antibody clone in a different buffer formulation.
In Western blot, Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (Anti-beta Tubulin antibody [EP1331Y] - Loading Control ab52901) staining at 1/1000 dilution.
All lanes: Western blot - Anti-Flagellin antibody [EPR28356-51] (Anti-Flagellin antibody [EPR28356-51] ab316010) at 1/1000 dilution
Lane 1: E. coli BL21(DE3) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa, 50 kDa
Exposure time: 180s
![Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (ab316011), expandable thumbnail](https://content.abcam.com/products/images/flagellin-antibody-epr28356-51-bsa-and-azide-free-ab316011--western-blot-img426206.jpg "Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (ab316011)")
Western blot - Anti-Flagellin antibody [EPR28356-51] - BSA and Azide free (ab316011)
Flagellin Western blot staining using rabbit Anti-Flagellin antibody
This data was developed using Anti-Flagellin antibody [EPR28356-51] ab316010, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with overexpressed Flagellin (Salmonella anatum), Flagellin (Salmonella typhimurium (strain LT2 /SGSC1412 / ATCC 700720)) and Flagellin (Salmonella paratyphi A (strain ATCC9150 / SARB42)).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Flagellin antibody [EPR28356-51] (Anti-Flagellin antibody [EPR28356-51] ab316010) at 1/1000 dilution
Lane 1: 293T cells transfected with a Flagellin (E.coli K12) expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with a Flagellin (Salmonella anatum) expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: 293T cells transfected with a Flagellin (Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)) expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: 293T cells transfected with a Flagellin (Salmonella paratyphi A (strain ATCC 9150 / SARB42)) expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa, 124 kDa
Exposure time: 15s
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