AB124714

Anti-FLAP antibody [EPR5640]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal FLAP antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

FLAP, ALOX5AP, Arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein

2 Images

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Western blot - Anti-FLAP antibody [EPR5640] (AB124714)

All lanes:

Western blot - Anti-FLAP antibody [EPR5640] (ab124714) at 1/1000 dilution

Lane 1:

Raji cell lysates at 10 µg

Lane 2:

THP1 cell lysates at 10 µg

Lane 3:

HepG2 cell lysates at 10 µg

Lane 4:

K562 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

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OI-RD Scanning - Anti-FLAP antibody [EPR5640] (AB124714)

OI-RD Scanning

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OI-RD Scanning - Anti-FLAP antibody [EPR5640] (AB124714)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-FLAP antibody [EPR5640] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5640

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FLAP also known as ALOX5AP is an important protein in the leukotriene biosynthetic pathway. It functions mechanically as a membrane-associated protein that facilitates the transfer of arachidonic acid to 5-lipoxygenase (5-LOX) a critical step in the production of leukotrienes. FLAP has a molecular mass of approximately 18 kDa and is primarily expressed in cells of the myeloid lineage such as neutrophils monocytes and macrophages.

Biological function summary

FLAP plays an essential role in the inflammatory response and is a component of the leukotriene synthesis complex. This complex is responsible for the production of leukotrienes which are potent lipid mediators that contribute to inflammation and immune regulation. By regulating leukotriene synthesis FLAP supports processes like chemotaxis cell aggregation and smooth muscle contraction essential for the body's response to inflammation.

Pathways

The FLAP signaling pathway is integral to the leukotriene metabolic pathway. It facilitates the interaction between arachidonic acid and 5-LOX leading to the generation of leukotrienes such as LTA4 and LTB4. These molecules are involved in numerous processes including bronchoconstriction and migration of leukocytes. Proteins related to FLAP within this pathway include 5-LOX and LTC4 synthase which further convert leukotrienes into biologically active compounds.

FLAP is associated with asthma and cardiovascular diseases. Its role in producing leukotrienes links it to the pathogenesis of inflammatory conditions. Elevated levels of leukotrienes mediated by FLAP can exacerbate symptoms in asthma by causing bronchial constriction. In cardiovascular diseases FLAP-related pathway dysregulation can contribute to atherosclerosis development. Proteins connected to FLAP through these disorders include leukotriene receptor antagonists which are therapeutic targets for reducing leukotriene-mediated effects.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

See full target information ALOX5AP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed38980302

2024

Uremic toxin indoxyl sulfate induces trained immunity via the AhR-dependent arachidonic acid pathway in end-stage renal disease (ESRD).

Applications

Unspecified application

Species

Unspecified reactive species

Hee Young Kim,Yeon Jun Kang,Dong Hyun Kim,Jiyeon Jang,Su Jeong Lee,Gwanghun Kim,Hee Byung Koh,Ye Eun Ko,Hyun Mu Shin,Hajeong Lee,Tae-Hyun Yoo,Won-Woo Lee

Cell death discovery 8:234 PubMed35477568

2022

Bone morphogenetic protein 4 alleviates nonalcoholic steatohepatitis by inhibiting hepatic ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xingchun Wang,Bingwei Ma,Xin Wen,Hui You,Chunjun Sheng,Le Bu,Shen Qu

Oncotarget 9:26751-26769 PubMed29928483

2018

miR-146a suppresses 5-lipoxygenase activating protein (FLAP) expression and Leukotriene B4 production in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph R Iacona,Nicholas J Monteleone,Carol S Lutz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FLAP antibody [EPR5640]

Western blot - Anti-FLAP antibody [EPR5640] (AB124714)

OI-RD Scanning - Anti-FLAP antibody [EPR5640] (AB124714)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise