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AB247977

Anti-FLAP antibody [EPR5640] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal FLAP antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FLAP, ALOX5AP, Arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein

2 Images
Western blot - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977)
  • WB

Unknown

Western blot - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977)

This data was developed using ab124714, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FLAP antibody [EPR5640] (<a href='/en-us/products/primary-antibodies/flap-antibody-epr5640-ab124714'>ab124714</a>) at 1/1000 dilution

Lane 1:

Raji cell lysates at 10 µg

Lane 2:

THP1 cell lysates at 10 µg

Lane 3:

HepG2 cell lysates at 10 µg

Lane 4:

K562 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

false

OI-RD Scanning - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5640

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab247977 is the carrier-free version of ab124714.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FLAP also known as ALOX5AP is an important protein in the leukotriene biosynthetic pathway. It functions mechanically as a membrane-associated protein that facilitates the transfer of arachidonic acid to 5-lipoxygenase (5-LOX) a critical step in the production of leukotrienes. FLAP has a molecular mass of approximately 18 kDa and is primarily expressed in cells of the myeloid lineage such as neutrophils monocytes and macrophages.
Biological function summary

FLAP plays an essential role in the inflammatory response and is a component of the leukotriene synthesis complex. This complex is responsible for the production of leukotrienes which are potent lipid mediators that contribute to inflammation and immune regulation. By regulating leukotriene synthesis FLAP supports processes like chemotaxis cell aggregation and smooth muscle contraction essential for the body's response to inflammation.

Pathways

The FLAP signaling pathway is integral to the leukotriene metabolic pathway. It facilitates the interaction between arachidonic acid and 5-LOX leading to the generation of leukotrienes such as LTA4 and LTB4. These molecules are involved in numerous processes including bronchoconstriction and migration of leukocytes. Proteins related to FLAP within this pathway include 5-LOX and LTC4 synthase which further convert leukotrienes into biologically active compounds.

FLAP is associated with asthma and cardiovascular diseases. Its role in producing leukotrienes links it to the pathogenesis of inflammatory conditions. Elevated levels of leukotrienes mediated by FLAP can exacerbate symptoms in asthma by causing bronchial constriction. In cardiovascular diseases FLAP-related pathway dysregulation can contribute to atherosclerosis development. Proteins connected to FLAP through these disorders include leukotriene receptor antagonists which are therapeutic targets for reducing leukotriene-mediated effects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
See full target information ALOX5AP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 77:100019 PubMed35397366

2022

IRF4 suppresses osteogenic differentiation of BM-MSCs by transcriptionally activating miR-636/DOCK9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuepu Zhang,Yue Zhang,Limin Yang,Yuexin Wu,Xiaohu Ma,Gang Tong,Zhaoliang Ban,Haosen Zhao
View all publications
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Product promise

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