Rabbit Recombinant Monoclonal FLAP antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977), expandable thumbnail](https://content.abcam.com/products/images/flap-antibody-epr5640-bsa-and-azide-free-ab247977--western-blot-img110667.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977)")
![OI-RD Scanning - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977), expandable thumbnail](https://content.abcam.com/products/images/flap-antibody-epr5640-bsa-and-azide-free-ab247977--oi-rd-scanning-img97708.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-FLAP antibody [EPR5640] - BSA and Azide free (AB247977)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
Alternative names
FLAP, ALOX5AP, Arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein
Recommended products
Rabbit Recombinant Monoclonal FLAP antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5640
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.66 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247977 is the carrier-free version of Anti-FLAP antibody [EPR5640] ab124714.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FLAP also known as ALOX5AP is an important protein in the leukotriene biosynthetic pathway. It functions mechanically as a membrane-associated protein that facilitates the transfer of arachidonic acid to 5-lipoxygenase (5-LOX) a critical step in the production of leukotrienes. FLAP has a molecular mass of approximately 18 kDa and is primarily expressed in cells of the myeloid lineage such as neutrophils monocytes and macrophages.
- Biological function summary
FLAP plays an essential role in the inflammatory response and is a component of the leukotriene synthesis complex. This complex is responsible for the production of leukotrienes which are potent lipid mediators that contribute to inflammation and immune regulation. By regulating leukotriene synthesis FLAP supports processes like chemotaxis cell aggregation and smooth muscle contraction essential for the body's response to inflammation.
- Pathways
The FLAP signaling pathway is integral to the leukotriene metabolic pathway. It facilitates the interaction between arachidonic acid and 5-LOX leading to the generation of leukotrienes such as LTA4 and LTB4. These molecules are involved in numerous processes including bronchoconstriction and migration of leukocytes. Proteins related to FLAP within this pathway include 5-LOX and LTC4 synthase which further convert leukotrienes into biologically active compounds.
- Associated diseases and disorders
FLAP is associated with asthma and cardiovascular diseases. Its role in producing leukotrienes links it to the pathogenesis of inflammatory conditions. Elevated levels of leukotrienes mediated by FLAP can exacerbate symptoms in asthma by causing bronchial constriction. In cardiovascular diseases FLAP-related pathway dysregulation can contribute to atherosclerosis development. Proteins connected to FLAP through these disorders include leukotriene receptor antagonists which are therapeutic targets for reducing leukotriene-mediated effects.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-FLAP antibody [EPR5640] - BSA and Azide free (ab247977), expandable thumbnail](https://content.abcam.com/products/images/flap-antibody-epr5640-bsa-and-azide-free-ab247977--western-blot-img110667.jpg "Western blot - Anti-FLAP antibody [EPR5640] - BSA and Azide free (ab247977)")
Western blot - Anti-FLAP antibody [EPR5640] - BSA and Azide free (ab247977)
This data was developed using Anti-FLAP antibody [EPR5640] ab124714, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-FLAP antibody [EPR5640] (Anti-FLAP antibody [EPR5640] ab124714) at 1/1000 dilution
Lane 1: Raji cell lysates at 10 µg
Lane 2: THP1 cell lysates at 10 µg
Lane 3: HepG2 cell lysates at 10 µg
Lane 4: K562 cell lysates at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
![OI-RD Scanning - Anti-FLAP antibody [EPR5640] - BSA and Azide free (ab247977), expandable thumbnail](https://content.abcam.com/products/images/flap-antibody-epr5640-bsa-and-azide-free-ab247977--oi-rd-scanning-img97708.jpg "OI-RD Scanning - Anti-FLAP antibody [EPR5640] - BSA and Azide free (ab247977)")
OI-RD Scanning - Anti-FLAP antibody [EPR5640] - BSA and Azide free (ab247977)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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