Rabbit Polyclonal FLASH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus.
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Publications
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- Nature structural & molecular biology 27:500-5102020CDK11 is required for transcription of replication-dependent histone genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavla Gajdušková et. al.PubMed 32367068
- Proceedings of the National Academy of Sciences of 103:14802-72006FLASH is an essential component of Cajal bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Barcaroli et. al.PubMed 17003126
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKL3
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-0.50000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
Alternative names
FLASH, KIAA1315, RIP25, CASP8AP2, CASP8-associated protein 2, FLICE-associated huge protein
Recommended products
Rabbit Polyclonal FLASH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKL3
- Purification technique
- Affinity purification
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FLASH also known as "CASP8 and FADD-like apoptosis regulator" is an important protein that plays a role in apoptosis. This protein has a molecular mass of approximately 220 kDa and can be found expressed in various tissues including the liver kidney and heart. FLASH localizes to the nucleus where it interacts with several nuclear components and contributes to critical cellular functions. Researchers recognize its significance in regulating apoptotic pathways making it a target for studies involving cell death.
- Biological function summary
The CASP8 and FADD-like apoptosis regulator is part of the complex involved in the regulation of apoptosis and cell cycle. FLASH plays its part by interacting with other apoptotic factors ensuring proper cell cycle progression and programmed cell death. It associates with the death effector domain (DED) proteins and has shown involvement in the activation of caspase-8. This regulation is significant for maintaining cellular homeostasis and preventing the development of cancerous cells.
- Pathways
Studies show that FLASH integrates into the apoptotic pathway and NF-kB signaling cascade. It engages in critical interactions with proteins such as FADD and caspase-8 which are essential for the initiation and execution of apoptosis. By influencing NF-kB signaling FLASH modulates inflammatory responses and survival signals effectively balancing cell death and survival mechanisms.
- Associated diseases and disorders
Research frequently connects FLASH to cancer and autoimmune diseases. Its dysregulation can lead to aberrant apoptotic signaling contributing to cancer progression or inappropriate cell death as seen in autoimmune conditions. FLASH’s interaction with proteins like caspase-8 makes it an attractive target for developing therapies for diseases where apoptosis is disrupted. Understanding FLASH’s function and interactions provides insight into potential therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-FLASH antibody (ab8420)
All lanes: Western blot - Anti-FLASH antibody (ab8420) at 0.5 µg/mL
All lanes: HeLa whole cell lysate
Predicted band size: 223 kDa
Observed band size: 220 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (ab8420)
ab8420 at 10μg/ml staining FLASH in Hela cells by ICC/IF
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