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AB8420

Anti-FLASH antibody

3

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal FLASH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus.

View Alternative Names

FLASH, KIAA1315, RIP25, CASP8AP2, CASP8-associated protein 2, FLICE-associated huge protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (AB8420)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (AB8420)

ab8420 at 10μg/ml staining FLASH in Hela cells by ICC/IF

Western blot - Anti-FLASH antibody (AB8420)
  • WB

Unknown

Western blot - Anti-FLASH antibody (AB8420)

All lanes:

Western blot - Anti-FLASH antibody (ab8420) at 0.5 µg/mL

All lanes:

HeLa whole cell lysate

Predicted band size: 223 kDa

Observed band size: 220 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CASP8AP2 aa 1950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UKL3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FLASH also known as "CASP8 and FADD-like apoptosis regulator" is an important protein that plays a role in apoptosis. This protein has a molecular mass of approximately 220 kDa and can be found expressed in various tissues including the liver kidney and heart. FLASH localizes to the nucleus where it interacts with several nuclear components and contributes to critical cellular functions. Researchers recognize its significance in regulating apoptotic pathways making it a target for studies involving cell death.
Biological function summary

The CASP8 and FADD-like apoptosis regulator is part of the complex involved in the regulation of apoptosis and cell cycle. FLASH plays its part by interacting with other apoptotic factors ensuring proper cell cycle progression and programmed cell death. It associates with the death effector domain (DED) proteins and has shown involvement in the activation of caspase-8. This regulation is significant for maintaining cellular homeostasis and preventing the development of cancerous cells.

Pathways

Studies show that FLASH integrates into the apoptotic pathway and NF-kB signaling cascade. It engages in critical interactions with proteins such as FADD and caspase-8 which are essential for the initiation and execution of apoptosis. By influencing NF-kB signaling FLASH modulates inflammatory responses and survival signals effectively balancing cell death and survival mechanisms.

Research frequently connects FLASH to cancer and autoimmune diseases. Its dysregulation can lead to aberrant apoptotic signaling contributing to cancer progression or inappropriate cell death as seen in autoimmune conditions. FLASH’s interaction with proteins like caspase-8 makes it an attractive target for developing therapies for diseases where apoptosis is disrupted. Understanding FLASH’s function and interactions provides insight into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in TNF-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B (PubMed : 12477726, PubMed : 15698540). Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation (PubMed : 12477726, PubMed : 15698540). Involved in TNF-induced activation of NF-kappa-B via a TRAF2-dependent pathway (By similarity). Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B (PubMed : 17245429). Required for the activation of CASP8 in FAS-mediated apoptosis (PubMed : 17245429). Involved for histone gene transcription and progression through S phase : required for the generation of mature histone mRNAs following the 3' end cleavage of histone pre-mRNAs (PubMed : 17003125, PubMed : 19854135, PubMed : 32367068).
See full target information CASP8AP2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 85:2487-2502.e12 PubMed40516529

2025

CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Ingham,Ignacio Alonso de Vega,Louise Morlot,William Gittens,Ivo A Hendriks,Ellen S Kakulidis,Raimundo Freire,Norman E Davey,Julien P Duxin,Michael Lund Nielsen,Niels Mailand

Nature structural & molecular biology 27:500-510 PubMed32367068

2020

CDK11 is required for transcription of replication-dependent histone genes.

Applications

Unspecified application

Species

Unspecified reactive species

Pavla Gajdušková,Igor Ruiz de Los Mozos,Michal Rájecký,Milan Hluchý,Jernej Ule,Dalibor Blazek

Proceedings of the National Academy of Sciences of 103:14802-7 PubMed17003126

2006

FLASH is an essential component of Cajal bodies.

Applications

Unspecified application

Species

Unspecified reactive species

D Barcaroli,D Dinsdale,M H Neale,L Bongiorno-Borbone,M Ranalli,E Munarriz,A E Sayan,J M McWilliam,T M Smith,E Fava,R A Knight,G Melino,V De Laurenzi
View all publications
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Product promise

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