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Mouse Monoclonal POLG antibody. Suitable for ICC/IF, WB, sELISA, ELISA and reacts with Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Zika virus, Japanese encephalitis virus, West Nile virus samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Dengue virus 1 Brazil/97-11/1997 POLG_DEN1B.
Alternative names=Genome polyprotein
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 89.9% RPMI 1640, 10% Fetal Bovine Serum (FBS)- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Full Length Protein corresponding to Dengue virus 1 Brazil/97-11/1997 POLG_DEN1B. Database link: P27909
Reactivity data
Select an application| ICC/IF | WB | sELISA | ELISA | |
|---|---|---|---|---|
| Dengue virus 1 | Tested | Tested | Tested | Expected |
| Dengue virus 2 | Tested | Tested | Tested | Expected |
| Dengue virus 3 | Tested | Tested | Tested | Expected |
| Dengue virus 4 | Tested | Tested | Tested | Expected |
| Japanese encephalitis virus | Expected | Tested | Tested | Expected |
| West Nile virus | Expected | Tested | Tested | Expected |
| Zika virus | Tested | Tested | Expected | Tested |
Images
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Dengue virus 1 | Dilution info - | Notes Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
Species Dengue virus 2 | Dilution info - | Notes Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
Species Dengue virus 3 | Dilution info - | Notes Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
Species Dengue virus 4 | Dilution info - | Notes Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
Species Zika virus | Dilution info - | Notes Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Japanese encephalitis virus, West Nile virus | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Dengue virus 1 | Dilution info - | Notes Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
Species Dengue virus 2 | Dilution info - | Notes Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
Species Dengue virus 3 | Dilution info - | Notes Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
Species Dengue virus 4 | Dilution info - | Notes Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
Species Zika virus | Dilution info - | Notes Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
Species Japanese encephalitis virus | Dilution info - | Notes Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
Species West Nile virus | Dilution info - | Notes Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zika virus | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zika virus | Dilution info - | Notes This monoclonal antibody has been primarily used for the capture/detection of DENV1, 2, 3, 4, ZIKV (data not shown), JEV and WNV antigens in an antigen capture ELISA. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus | Dilution info Use at an assay dependent concentration. | Notes - |
Recommended products
Mouse Monoclonal POLG antibody. Suitable for ICC/IF, WB, sELISA, ELISA and reacts with Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Zika virus, Japanese encephalitis virus, West Nile virus samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Dengue virus 1 Brazil/97-11/1997 POLG_DEN1B.
Alternative names=Genome polyprotein
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 89.9% RPMI 1640, 10% Fetal Bovine Serum (FBS)- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Full Length Protein corresponding to Dengue virus 1 Brazil/97-11/1997 POLG_DEN1B. Database link: P27909
- Clone number
HF8-1/C1-6/E12
- Purity
Tissue culture supernatant
- Specificity
ab214335 reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV.
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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