JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122011

Anti-FLG2 antibody

2

(1 Review)

|

(4 Publications)

Rabbit Polyclonal FLG2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FLG2 aa 250-400.

View Alternative Names

IFPS, FLG2, Filaggrin-2, FLG-2, Intermediate filament-associated and psoriasis-susceptibility protein, Ifapsoriasin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLG2 antibody (AB122011)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLG2 antibody (AB122011)

ab122011, staining FLG2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-FLG2 antibody (AB122011)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FLG2 antibody (AB122011)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122011 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FLG2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q5D862

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FLG2 also known as Filaggrin-2 is a protein involved in skin barrier function. It weighs approximately 248 kilodaltons and expresses mainly in the epidermis particularly in the stratum corneum. Its structure includes several repeat units similar in domain composition to the filaggrin protein which indicates its important roles in epidermal differentiation and hydration.
Biological function summary

The FLG2 protein provides structural support essential for skin integrity and contributes to preventing water loss. It associates with keratin filaments as part of the epidermal cornified envelope. The protein helps maintain skin hydration by breaking down into free amino acids which improve skin moisture. FLG2 interacts with other proteins like filaggrin suggesting overlapping functions in skin homeostasis.

Pathways

The FLG2 protein significantly impacts the skin barrier formation and repair processes. It participates in the epidermal differentiation complex (EDC) pathway and the key hydration pathway. FLG2 shares functional pathways with the filaggrin protein which also plays a role in forming the skin barrier by aggregating keratin filaments and releasing amino acids that maintain hydration and acid mantle of the skin.

FLG2 associates with skin conditions such as atopic dermatitis and ichthyosis vulgaris. Mutations or reduced expression in the FLG2 gene compromise the skin barrier leading to increased susceptibility to these conditions. In the context of atopic dermatitis its interaction with the filaggrin protein becomes apparent as both proteins being together or separately altered can exacerbate barrier defects highlighting their essential role in maintaining skin health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Essential for normal cell-cell adhesion in the cornified cell layers (PubMed : 29758285). Important for proper integrity and mechanical strength of the stratum corneum of the epidermis (PubMed : 29505760).
See full target information FLG2
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Asian journal of andrology 27:245-253 PubMed39686771

2024

Novel homozygous SPAG17 variants cause human male infertility through multiple morphological abnormalities of spermatozoal flagella related to axonemal microtubule doublets.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Fazal Rahim,Meng-Lei Yang,Meftah Uddin,Jing-Wei Ye,Imtiaz Ali,Yousaf Raza,Abu Mansoor,Muhammad Shoaib,Mujahid Hussain,Ihsan Khan,Basit Shah,Asad Khan,Ahmad Nisar,Hui Ma,Bo Xu,Wasim Shah,Qing-Hua Shi

JID innovations : skin science from molecules to population health 4:100284 PubMed38859976

2024

Uncommon Variants in and Are Associated with Atopic Dermatitis in the Ethiopian Population.

Applications

Unspecified application

Species

Unspecified reactive species

Sailan Wang,Julia K Elmgren,Jesper Eisfeldt,Samina Asad,Marlene Ek,Kassahun Bilcha,Annisa Befekadu,Carl-Fredrik Wahlgren,Magnus Nordenskjöld,Fulya Taylan,Isabel Tapia-Paez,Maria Bradley

ImmunoHorizons 5:830-843 PubMed34702760

2021

Transcriptomic Analysis of Healthy and Atopic Dermatitis Samples Reveals the Role of IL-37 in Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajun Zhou,David C Gemperline,Matthew J Turner,Jonathan Oldach,Jennifer Molignano,Jonathan T Sims,Keith R Stayrook

The Journal of allergy and clinical immunology 145:619-631.e2 PubMed31783056

2019

Skin barrier damage after exposure to paraphenylenediamine.

Applications

Unspecified application

Species

Unspecified reactive species

Sanne S Meisser,Can Altunbulakli,Josefine Bandier,Morten S Opstrup,Francesc Castro-Giner,Mübeccel Akdis,Charlotte M Bonefeld,Jeanne D Johansen,Cezmi A Akdis
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB221155

Anti-Filaggrin antibody [EPR21892]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-Filaggrin antibody [EPR21892] (AB221155)

  • 5
  • 3
Primary Antibodies

AB198994

Anti-Loricrin antibody [EPR7149(2)] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994)

  • 5
  • 3
Primary Antibodies

AB181980

Anti-Involucrin antibody [EPR13054]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (AB181980)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com