AB180902

Anti-FLI1 antibody - C-terminal

Name: Anti-FLI1 antibody - C-terminal (ab180902) | Abcam
Brand: Abcam Limited
SKU: AB180902

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(5 Publications)

Rabbit Polyclonal FLI1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FLI1.

View Alternative Names

Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-FLI1 antibody - C-terminal (AB180902)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FLI1 antibody - C-terminal (AB180902)

Immunofluorescent analysis of HeLa cells labeling FLI1 with ab180902 at 5 μg/ml.

Supplier Data

Western blot - Anti-FLI1 antibody - C-terminal (AB180902)

Lane 1:

Western blot - Anti-FLI1 antibody - C-terminal (ab180902) at 0.5 µg/mL

Lane 2:

Western blot - Anti-FLI1 antibody - C-terminal (ab180902) at 1 µg/mL

All lanes:

Jurkat cell lysate at 15 µg

Predicted band size: 51 kDa

Observed band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human FLI1. The exact immunogen used to generate this antibody is proprietary information.

Q01543

Specificity

At least four isoforms of FLI1 are known to exist; this antibody will detect all four.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FLI1 protein also known as Friend leukemia virus integration 1 acts as a transcription factor. It consists of 452 amino acids resulting in a molecular mass of about 50 kDa. FLI1 is part of the ETS (E26 transformation-specific) family of transcription factors and it binds to specific DNA sequences to regulate the expression of various genes. It expresses in hematopoietic cells endothelial cells and several tissues during development. High expression levels in certain tissues suggest a significant role in diverse cellular functions.

Biological function summary

The FLI1 protein influences cell proliferation differentiation and apoptosis. It also plays a role in the regulation of genes involved in the immune response. FLI1 forms complexes with other proteins to exert its function therefore impacting gene expression in cellular contexts. Its participation in these processes indicates its regulatory impact on normal cellular development and function.

Pathways

FLI1 interacts with signaling networks that govern the hematopoietic lineage and endothelial cell function. It participates in the MAPK/ERK pathway influencing cell cycle and growth signaling. Through these pathways FLI1 has functional interactions with proteins such as GATA1 and RUNX1 establishing its importance in vascular development and hematopoiesis.

FLI1 has significant associations with Ewing sarcoma and certain leukemias. In Ewing sarcoma chromosomal translocations involve FLI1 leading to oncogenic fusion proteins that drive tumorigenesis. It also interacts with partners like EWSR1 in this context. Moreover abnormal levels of FLI1 relate to blood disorders due to its role in hematopoietic regulation. Understanding these interactions and associations aids in developing therapeutic strategies targeting these illnesses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcriptional activator (PubMed : 24100448, PubMed : 26316623, PubMed : 28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

See full target information FLI1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 110:2792-2798 PubMed40468949

2025

Characterization of a novel mutation in a family with thrombocytopenia and other congenital malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Ammeti,Serena Barozzi,Alessandro Pecci,Melania Eva Zanchetta,Edward Cesnik,Alessandra Ferlini,Mariabeatrice Sanchini,Laura Verga,Valeria Bozzi,Fabio Corsolini,Michela Faleschini,Anna Savoia,Stefania Bigoni

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2004846 PubMed34060252

2021

SPOP and OTUD7A Control EWS-FLI1 Protein Stability to Govern Ewing Sarcoma Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Su,Jianfeng Chen,Yao Jiang,Ying Wang,Tamara Vital,Jiaming Zhang,Christian Laggner,Kong T Nguyen,Zhichuan Zhu,Alex W Prevatte,Natalie K Barker,Laura E Herring,Ian J Davis,Pengda Liu

Oncology letters 15:2795-2800 PubMed29435006

2018

PRL-3 promotes breast cancer progression by downregulating p14-mediated p53 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Xie,Hao Wang

Annals of the rheumatic diseases 76:924-934 PubMed28062404

2017

Endothelial-to-mesenchymal transition contributes to endothelial dysfunction and dermal fibrosis in systemic sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Manetti,Eloisa Romano,Irene Rosa,Serena Guiducci,Silvia Bellando-Randone,Amato De Paulis,Lidia Ibba-Manneschi,Marco Matucci-Cerinic

Annals of the rheumatic diseases 75:1541-9 PubMed26359450

2015

Decreased expression of neuropilin-1 as a novel key factor contributing to peripheral microvasculopathy and defective angiogenesis in systemic sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eloisa Romano,Inês Chora,Mirko Manetti,Celestina Mazzotta,Irene Rosa,Silvia Bellando-Randone,Jelena Blagojevic,Raquel Soares,Jerôme Avouac,Yannick Allanore,Lidia Ibba-Manneschi,Marco Matucci-Cerinic,Serena Guiducci

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FLI1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-FLI1 antibody - C-terminal (AB180902)

Western blot - Anti-FLI1 antibody - C-terminal (AB180902)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise