AB124791

Anti-FLI1 antibody [EPR4645]

Name: Anti-FLI1 antibody [EPR4645] (ab124791) | Abcam
Brand: Abcam Limited
SKU: AB124791
Price: 330 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal FLI1 antibody. Suitable for WB and reacts with Recombinant fragment, Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1

2 Images

Unknown

Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)

All lanes:

Western blot - Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

Lane 1:

Raw264.7 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

false

Unknown

Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)

All lanes:

Western blot - Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

Lane 1:

ERG recombinant protein at 0.01 µg

Lane 2:

FLI1 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

false

Carrier free

Anti-FLI1 antibody [EPR4645] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4645

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This product does not cross-react with ERG.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FLI1 protein also known as Friend leukemia virus integration 1 acts as a transcription factor. It consists of 452 amino acids resulting in a molecular mass of about 50 kDa. FLI1 is part of the ETS (E26 transformation-specific) family of transcription factors and it binds to specific DNA sequences to regulate the expression of various genes. It expresses in hematopoietic cells endothelial cells and several tissues during development. High expression levels in certain tissues suggest a significant role in diverse cellular functions.

Biological function summary

The FLI1 protein influences cell proliferation differentiation and apoptosis. It also plays a role in the regulation of genes involved in the immune response. FLI1 forms complexes with other proteins to exert its function therefore impacting gene expression in cellular contexts. Its participation in these processes indicates its regulatory impact on normal cellular development and function.

Pathways

FLI1 interacts with signaling networks that govern the hematopoietic lineage and endothelial cell function. It participates in the MAPK/ERK pathway influencing cell cycle and growth signaling. Through these pathways FLI1 has functional interactions with proteins such as GATA1 and RUNX1 establishing its importance in vascular development and hematopoiesis.

FLI1 has significant associations with Ewing sarcoma and certain leukemias. In Ewing sarcoma chromosomal translocations involve FLI1 leading to oncogenic fusion proteins that drive tumorigenesis. It also interacts with partners like EWSR1 in this context. Moreover abnormal levels of FLI1 relate to blood disorders due to its role in hematopoietic regulation. Understanding these interactions and associations aids in developing therapeutic strategies targeting these illnesses.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Sequence-specific transcriptional activator (PubMed : 24100448, PubMed : 26316623, PubMed : 28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

See full target information FLI1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 110:2792-2798 PubMed40468949

2025

Characterization of a novel mutation in a family with thrombocytopenia and other congenital malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Ammeti,Serena Barozzi,Alessandro Pecci,Melania Eva Zanchetta,Edward Cesnik,Alessandra Ferlini,Mariabeatrice Sanchini,Laura Verga,Valeria Bozzi,Fabio Corsolini,Michela Faleschini,Anna Savoia,Stefania Bigoni

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 74:28 PubMed39862276

2025

Pericytes mediate neuroinflammation via Fli-1 in endotoxemia and sepsis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Li,Liu Liu,Perry V Halushka,Maria Trojanowska,Guirong Wang,Adviye Ergul,Hongkuan Fan

International journal of molecular sciences 25: PubMed39201581

2024

Antibody to Endogenous Cardiotonic Steroid Reverses Vascular Fibrosis and Restores Vasorelaxation in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia I Agalakova,Elena V Mikhailova,Ivan A Ershov,Olga V Nadei,Arseny A Pyankov,Michael M Galagoudza,C David Adair,Irina V Romanova,Alexei Y Bagrov

Kidney diseases (Basel, Switzerland) 8:424-435 PubMed36466072

2022

SENP3 Aggravates Renal Tubular Epithelial Cell Apoptosis in Lipopolysaccharide-Induced Acute Kidney Injury via deSUMOylation of Drp1.

Applications

Unspecified application

Species

Unspecified reactive species

Lirui Wang,Jiangtao Li,Chen Yu

Nature chemical biology 16:50-59 PubMed31819276

2019

CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan T Ross,Felix Lohmann,Seth Carbonneau,Aleem Fazal,Wilhelm A Weihofen,Scott Gleim,Michael Salcius,Frederic Sigoillot,Martin Henault,Sarah H Carl,Juan B Rodríguez-Molina,Howard R Miller,Scott M Brittain,Jason Murphy,Mark Zambrowski,Geoffrey Boynton,Yuan Wang,Aye Chen,Gregory J Molind,Johannes H Wilbertz,Caroline G Artus-Revel,Min Jia,Favour A Akinjiyan,Jonathan Turner,Judith Knehr,Walter Carbone,Sven Schuierer,John S Reece-Hoyes,Kevin Xie,Chitra Saran,Eric T Williams,Guglielmo Roma,Matt Spencer,Jeremy Jenkins,Elizabeth L George,Jason R Thomas,Gregory Michaud,Markus Schirle,John Tallarico,Lori A Passmore,Jeffrey A Chao,Rohan E J Beckwith

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FLI1 antibody [EPR4645]

Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)

Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise