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Anti-FLI1 antibody [EPR4646] ab133485 is a rabbit monoclonal antibody that is used in FLI1 western blotting and IHC. Suitable for human and mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR4646 is the most widely used clone for FLI1 on the market and is cited in >30 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation


Images

Western blot - Anti-FLI1 antibody [EPR4646] (AB133485), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (AB133485), expandable thumbnail
  • Western blot - Anti-FLI1 antibody [EPR4646] (AB133485), expandable thumbnail
  • Western blot - Anti-FLI1 antibody [EPR4646] (AB133485), expandable thumbnail
  • Western blot - Anti-FLI1 antibody [EPR4646] (AB133485), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPICC/IFFlow CytChIC/CUT&RUN-seq
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Expected
Tested
Not recommended
Not recommended
Not recommended
Expected
Recombinant full length protein - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/700

Notes

For unpurified, use 1/140.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

1/1000

Notes

For unpurified, use 1/200.

Species

Mouse

Dilution info

1/1000

Notes

For unpurified, use 1/200.

Species

Human

Dilution info

1/1000

Notes

For unpurified, use 1/200.

Not recommended
Not recommended

Species

Mouse, Human, Recombinant full length protein - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Recombinant full length protein - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

-

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Sequence-specific transcriptional activator (PubMed:24100448, PubMed:26316623, PubMed:28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Alternative names

Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1

Recommended products

Anti-FLI1 antibody [EPR4646] ab133485 is a rabbit monoclonal antibody that is used in FLI1 western blotting and IHC. Suitable for human and mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR4646 is the most widely used clone for FLI1 on the market and is cited in >30 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4646

Purification technique

Affinity purification Protein A

Specificity

This antibody does not cross-react with ERG.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The FLI1 protein also known as Friend leukemia virus integration 1 acts as a transcription factor. It consists of 452 amino acids resulting in a molecular mass of about 50 kDa. FLI1 is part of the ETS (E26 transformation-specific) family of transcription factors and it binds to specific DNA sequences to regulate the expression of various genes. It expresses in hematopoietic cells endothelial cells and several tissues during development. High expression levels in certain tissues suggest a significant role in diverse cellular functions.

Biological function summary

The FLI1 protein influences cell proliferation differentiation and apoptosis. It also plays a role in the regulation of genes involved in the immune response. FLI1 forms complexes with other proteins to exert its function therefore impacting gene expression in cellular contexts. Its participation in these processes indicates its regulatory impact on normal cellular development and function.

Pathways

FLI1 interacts with signaling networks that govern the hematopoietic lineage and endothelial cell function. It participates in the MAPK/ERK pathway influencing cell cycle and growth signaling. Through these pathways FLI1 has functional interactions with proteins such as GATA1 and RUNX1 establishing its importance in vascular development and hematopoiesis.

Associated diseases and disorders

FLI1 has significant associations with Ewing sarcoma and certain leukemias. In Ewing sarcoma chromosomal translocations involve FLI1 leading to oncogenic fusion proteins that drive tumorigenesis. It also interacts with partners like EWSR1 in this context. Moreover abnormal levels of FLI1 relate to blood disorders due to its role in hematopoietic regulation. Understanding these interactions and associations aids in developing therapeutic strategies targeting these illnesses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution

    All lanes: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

    Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-FLI1 antibody [EPR4646] (ab133485) at 1/200 dilution

    All lanes: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

    All lanes: Western blot - Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution

    Lane 1: U937 cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: Raw264.7 cell lysate at 10 µg

    Lane 4: HL-60 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 51 kDa

  • Western blot - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Western blot - Anti-FLI1 antibody [EPR4646] (ab133485)

    All lanes: Western blot - Anti-FLI1 antibody [EPR4646] (ab133485) at 1/1000 dilution

    Lane 1: FLI1 recombinant protein at 0.01 µg

    Lane 2: ERGrecombinant protein at 0.01 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

    Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

    Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (ab133485)

    Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling FLI1 with ab133485 at a concentration of 0.01 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32mins.

    ab133485 Anti-FLI1 antibody [EPR4646] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

  • ChIC/CUT&RUN sequencing - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-FLI1 antibody [EPR4646] (ab133485)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 A-673 (human muscle Ewings sarcoma cell line) cells and 5 µg of ab133485 [EPR4646]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-FLI1 antibody [EPR4646] (ab133485)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 A-673 (human muscle Ewings sarcoma cell line) cells and 5 µg of ab133485 [EPR4646]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-FLI1 antibody [EPR4646] (ab133485), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-FLI1 antibody [EPR4646] (ab133485)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 A-673 (human muscle Ewings sarcoma cell line) cells and 5 µg of ab133485 [EPR4646]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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