Rabbit Recombinant Monoclonal Flightless I antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692), expandable thumbnail](https://content.abcam.com/products/images/flightless-i-antibody-epr4201-bsa-and-azide-free-ab247692--western-blot-img111228.gif "Western blot - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)")
![Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692), expandable thumbnail](https://content.abcam.com/products/images/flightless-i-antibody-epr4201-bsa-and-azide-free-ab247692--immunocytochemistry-immunofluorescence-img33814.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Expected |
| Mouse | Not recommended | Predicted | Predicted | Predicted |
| Rat | Not recommended | Predicted | Predicted | Predicted |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
Alternative names
Protein flightless-1 homolog, FLII, FLIL
Recommended products
Rabbit Recombinant Monoclonal Flightless I antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Alternative names
Protein flightless-1 homolog, FLII, FLIL
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR4201
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab247692 is the carrier-free version of Anti-Flightless I antibody [EPR4201] ab108594.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flightless I also known as FLII is a multifunctional protein with a mass of approximately 131 kDa. This protein belongs to the gelsolin family and contains leucine-rich repeats (LRR) and a partially functional gelsolin domain. It is widely expressed in various tissues including muscle and skin. Functionally Flightless I interacts with actin filaments and plays a role in modulating the actin cytoskeleton. Through its actin-binding property Flightless I participates in cellular processes such as motility and structural maintenance.
- Biological function summary
Flightless I protein influences several cell mechanisms by associating with signaling complexes. It functions as a part of cellular assemblies where it modulates actin dynamics and affects transcriptional activities by interacting with other cellular components. Additionally Flightless I contributes to cell growth survival and wound healing processes. The protein can also regulate certain gene expressions by acting as a transcriptional co-regulator highlighting its involvement in cellular communication.
- Pathways
Flightless I has significant roles in cellular signaling and cytoskeletal networks. It is actively involved in the Wnt signaling pathway as well as the Toll-like receptor signaling pathway. These pathways are central to cell communication and immune response regulation. Flightless I operates alongside proteins such as Dishevelled and MyD88 integrating signals that influence cell behavior and immune reactions. By mediating these interactions Flightless I enhances the cellular response to environmental cues.
- Associated diseases and disorders
Flightless I is associated with aberrant conditions like cancer and muscular dystrophy. Its dysregulation has been linked to tumor progression and metastasis where it influences cell migration and invasion. Flightless I interacts with proteins such as beta-catenin and dystrophin in these contexts affecting cellular adhesion and stability. Understanding these interactions can provide insights into therapeutic targeting strategies for managing and treating associated pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692)
This data was developed using Anti-Flightless I antibody [EPR4201] ab108594, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Flightless I antibody [EPR4201] (Anti-Flightless I antibody [EPR4201] ab108594) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: A549 cell lysate at 10 µg
Lane 3: A431 cell lysate at 10 µg
Predicted band size: 145 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692), expandable thumbnail](https://content.abcam.com/products/images/flightless-i-antibody-epr4201-bsa-and-azide-free-ab247692--immunocytochemistry-immunofluorescence-img33814.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692)")
Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692)
This data was developed using Anti-Flightless I antibody [EPR4201] ab108594, the same antibody clone in a different buffer formulation.
Anti-Flightless I antibody [EPR4201] ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692), expandable thumbnail](https://content.abcam.com/products/images/flightless-i-antibody-epr4201-bsa-and-azide-free-ab247692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107138.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (ab247692)
This data was developed using Anti-Flightless I antibody [EPR4201] ab108594, the same antibody clone in a different buffer formulation.
Anti-Flightless I antibody [EPR4201] ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissuePerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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