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AB247692

Anti-Flightless I antibody [EPR4201] - BSA and Azide free

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Rabbit Recombinant Monoclonal Flightless I antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

View Alternative Names

FLIL, FLII, Protein flightless-1 homolog

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)

This data was developed using ab108594, the same antibody clone in a different buffer formulation.

ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)

This data was developed using ab108594, the same antibody clone in a different buffer formulation.

ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissuePerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)
  • WB

Unknown

Western blot - Anti-Flightless I antibody [EPR4201] - BSA and Azide free (AB247692)

This data was developed using ab108594, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Flightless I antibody [EPR4201] (<a href='/en-us/products/primary-antibodies/flightless-i-antibody-epr4201-ab108594'>ab108594</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Predicted band size: 145 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4201

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab247692 is the carrier-free version of ab108594.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flightless I also known as FLII is a multifunctional protein with a mass of approximately 131 kDa. This protein belongs to the gelsolin family and contains leucine-rich repeats (LRR) and a partially functional gelsolin domain. It is widely expressed in various tissues including muscle and skin. Functionally Flightless I interacts with actin filaments and plays a role in modulating the actin cytoskeleton. Through its actin-binding property Flightless I participates in cellular processes such as motility and structural maintenance.
Biological function summary

Flightless I protein influences several cell mechanisms by associating with signaling complexes. It functions as a part of cellular assemblies where it modulates actin dynamics and affects transcriptional activities by interacting with other cellular components. Additionally Flightless I contributes to cell growth survival and wound healing processes. The protein can also regulate certain gene expressions by acting as a transcriptional co-regulator highlighting its involvement in cellular communication.

Pathways

Flightless I has significant roles in cellular signaling and cytoskeletal networks. It is actively involved in the Wnt signaling pathway as well as the Toll-like receptor signaling pathway. These pathways are central to cell communication and immune response regulation. Flightless I operates alongside proteins such as Dishevelled and MyD88 integrating signals that influence cell behavior and immune reactions. By mediating these interactions Flightless I enhances the cellular response to environmental cues.

Flightless I is associated with aberrant conditions like cancer and muscular dystrophy. Its dysregulation has been linked to tumor progression and metastasis where it influences cell migration and invasion. Flightless I interacts with proteins such as beta-catenin and dystrophin in these contexts affecting cellular adhesion and stability. Understanding these interactions can provide insights into therapeutic targeting strategies for managing and treating associated pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a regulator of actin polymerization, required for proper myofibril organization and regulation of the length of sarcomeric thin filaments (By similarity). It also plays a role in the assembly of cardiomyocyte cell adhesion complexes (By similarity). Regulates cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation (By similarity). May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1 (PubMed : 14966289). Involved in estrogen hormone signaling.
See full target information FLII
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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