AB109015

Anti-Flightless I antibody [EPR4202(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Flightless I antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FLIL, FLII, Protein flightless-1 homolog

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

Immunohistochemical analysis of Flightless 1 expression in paraffin-embedded Human muscle tissue using ab109015 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

Immunofluorescent staining of Flightless I in HeLa cells using ab109015 at 1/100 dilution.

Unknown

Western blot - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

All lanes:

Western blot - Anti-Flightless I antibody [EPR4202(2)] (ab109015) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Predicted band size: 145 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4202(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flightless I also known as FLII is a multifunctional protein with a mass of approximately 131 kDa. This protein belongs to the gelsolin family and contains leucine-rich repeats (LRR) and a partially functional gelsolin domain. It is widely expressed in various tissues including muscle and skin. Functionally Flightless I interacts with actin filaments and plays a role in modulating the actin cytoskeleton. Through its actin-binding property Flightless I participates in cellular processes such as motility and structural maintenance.

Biological function summary

Flightless I protein influences several cell mechanisms by associating with signaling complexes. It functions as a part of cellular assemblies where it modulates actin dynamics and affects transcriptional activities by interacting with other cellular components. Additionally Flightless I contributes to cell growth survival and wound healing processes. The protein can also regulate certain gene expressions by acting as a transcriptional co-regulator highlighting its involvement in cellular communication.

Pathways

Flightless I has significant roles in cellular signaling and cytoskeletal networks. It is actively involved in the Wnt signaling pathway as well as the Toll-like receptor signaling pathway. These pathways are central to cell communication and immune response regulation. Flightless I operates alongside proteins such as Dishevelled and MyD88 integrating signals that influence cell behavior and immune reactions. By mediating these interactions Flightless I enhances the cellular response to environmental cues.

Flightless I is associated with aberrant conditions like cancer and muscular dystrophy. Its dysregulation has been linked to tumor progression and metastasis where it influences cell migration and invasion. Flightless I interacts with proteins such as beta-catenin and dystrophin in these contexts affecting cellular adhesion and stability. Understanding these interactions can provide insights into therapeutic targeting strategies for managing and treating associated pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Is a regulator of actin polymerization, required for proper myofibril organization and regulation of the length of sarcomeric thin filaments (By similarity). It also plays a role in the assembly of cardiomyocyte cell adhesion complexes (By similarity). Regulates cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation (By similarity). May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1 (PubMed : 14966289). Involved in estrogen hormone signaling.

See full target information FLII

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 13:e7286 PubMed38803199

2024

Gelsolin knockdown confers radiosensitivity to glioblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Tao Jiang,Yanwei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Flightless I antibody [EPR4202(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

Western blot - Anti-Flightless I antibody [EPR4202(2)] (AB109015)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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