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AB247746

Anti-Flightless I antibody [EPR4202(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Flightless I antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

FLIL, FLII, Protein flightless-1 homolog

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] - BSA and Azide free (AB247746)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] - BSA and Azide free (AB247746)

This data was developed using ab109015, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Flightless 1 expression in paraffin-embedded Human muscle tissue using ab109015 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] - BSA and Azide free (AB247746)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] - BSA and Azide free (AB247746)

This data was developed using ab109015, the same antibody clone in a different buffer formulation.Immunofluorescent staining of Flightless I in HeLa cells using ab109015 at 1/100 dilution.

Western blot - Anti-Flightless I antibody [EPR4202(2)] - BSA and Azide free (AB247746)
  • WB

Unknown

Western blot - Anti-Flightless I antibody [EPR4202(2)] - BSA and Azide free (AB247746)

This data was developed using ab109015, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Flightless I antibody [EPR4202(2)] (<a href='/en-us/products/primary-antibodies/flightless-i-antibody-epr42022-ab109015'>ab109015</a>) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Predicted band size: 145 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4202(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247746 is the carrier-free version of ab109015.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flightless I also known as FLII is a multifunctional protein with a mass of approximately 131 kDa. This protein belongs to the gelsolin family and contains leucine-rich repeats (LRR) and a partially functional gelsolin domain. It is widely expressed in various tissues including muscle and skin. Functionally Flightless I interacts with actin filaments and plays a role in modulating the actin cytoskeleton. Through its actin-binding property Flightless I participates in cellular processes such as motility and structural maintenance.
Biological function summary

Flightless I protein influences several cell mechanisms by associating with signaling complexes. It functions as a part of cellular assemblies where it modulates actin dynamics and affects transcriptional activities by interacting with other cellular components. Additionally Flightless I contributes to cell growth survival and wound healing processes. The protein can also regulate certain gene expressions by acting as a transcriptional co-regulator highlighting its involvement in cellular communication.

Pathways

Flightless I has significant roles in cellular signaling and cytoskeletal networks. It is actively involved in the Wnt signaling pathway as well as the Toll-like receptor signaling pathway. These pathways are central to cell communication and immune response regulation. Flightless I operates alongside proteins such as Dishevelled and MyD88 integrating signals that influence cell behavior and immune reactions. By mediating these interactions Flightless I enhances the cellular response to environmental cues.

Flightless I is associated with aberrant conditions like cancer and muscular dystrophy. Its dysregulation has been linked to tumor progression and metastasis where it influences cell migration and invasion. Flightless I interacts with proteins such as beta-catenin and dystrophin in these contexts affecting cellular adhesion and stability. Understanding these interactions can provide insights into therapeutic targeting strategies for managing and treating associated pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a regulator of actin polymerization, required for proper myofibril organization and regulation of the length of sarcomeric thin filaments (By similarity). It also plays a role in the assembly of cardiomyocyte cell adhesion complexes (By similarity). Regulates cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation (By similarity). May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1 (PubMed : 14966289). Involved in estrogen hormone signaling.
See full target information FLII
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Product promise

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For full details, please see our Terms & Conditions

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