AB6144

Anti-FLIP antibody

Name: Anti-FLIP antibody (ab6144) | Abcam
Brand: Abcam Limited
SKU: AB6144
Rating: 2 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal FLIP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CFLAR aa 400-500.

View Alternative Names

CASH, CASP8AP1, CLARP, MRIT, CFLAR, CASP8 and FADD-like apoptosis regulator, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, Inhibitor of FLICE, MACH-related inducer of toxicity, Usurpin, Casper, c-FLIP, FLAME-1, I-FLICE

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB6144)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB6144)

ab6144 at 10μg/ml staining FLIP in Jurkat cells by ICC/IF

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB6144)

Immunofluorescence of FLIP in Jurkat cells using ab6144 at 10 ug/ml.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB6144)

ab6144 at 5μg/ml staining FLIP in K562 cells by ICC/IF (Immunocytochemistry/Immunofluorescence).

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Western blot - Anti-FLIP antibody (AB6144)

All lanes:

Western blot - Anti-FLIP antibody (ab6144) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate

Lane 2:

Jurkat whole cell lysate

Lane 3:

K562 whole cell lysate

Predicted band size: 55 kDa

Observed band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CFLAR aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

O15519

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified IgG prepared by ion exchange chromatography.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FLIP also known as c-FLIP or CASP8 and FADD-like apoptosis regulator is an important protein that inhibits apoptosis. It has a molecular mass of around 55-58 kDa and comes in multiple isoforms including c-FLIP(L) and c-FLIP(S). FLIP is expressed in many tissues such as the thymus spleen and lungs and plays a role in immune regulation by modulating apoptosis induced by death receptors like Fas and TNFR1. Its regulation of cell death makes it key to cell survival especially in the context of immune cells.

Biological function summary

FLIP protein impacts cellular processes by blocking the activation of caspase-8 preventing the apoptosis cascade from proceeding. It is not typically part of a large complex but interacts directly with the death-inducing signaling complex (DISC). By inhibiting the apoptotic machinery it protects cells from unnecessary death signals facilitating controlled cellular turnover and immune responses.

Pathways

FLIP affects apoptosis and NF-kB signaling. These pathways are vital for cell survival inflammation and immune responses. FLIP interacts closely with Fas-associated protein with death domain (FADD) and caspase-8 to regulate apoptotic pathways. It acts as a checkpoint in intrinsic and extrinsic apoptosis pathways thereby influencing both normal and pathological processes such as immune cell survival and proliferation.

FLIP expression connects to cancer and autoimmune disorders. Its overexpression can contribute to cancer progression by allowing cancer cells to escape apoptosis leading to uncontrolled cell growth. Furthermore it can be linked to autoimmune disorders where inappropriate control of apoptosis results in prolonged survival of autoreactive immune cells. In these contexts FLIP’s interaction with proteins such as Fas and TNFR1 becomes significant highlighting its potential as a therapeutic target in mitigating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells (PubMed : 31046799). Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

See full target information CFLAR

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34029184

2021

A phosphorylation of RIPK3 kinase initiates an intracellular apoptotic pathway that promotes prostaglandin-induced corpus luteum regression.

Applications

Unspecified application

Species

Unspecified reactive species

Dianrong Li,Jie Chen,Jia Guo,Lin Li,Gaihong Cai,She Chen,Jia Huang,Hui Yang,Yinhua Zhuang,Fengchao Wang,Xiaodong Wang

International journal of oncology 53:2258-2268 PubMed30132512

2018

β-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng You,Shimin Zhang,Yingming Sun,Shiyu Zhang,Guiliang Tang,Fang Tang,Xuefeng Liu,Yu Xiao,Junhong Zhang,Yan Gong,Conghua Xie

Bioscience reports 38: PubMed29724888

2018

hnRNP K plays a protective role in TNF-α-induced apoptosis in podocytes.

Applications

Species

Unspecified reactive species

Shili Zhao,Junxia Feng,Qi Wang,Lu Tian,Yunfang Zhang,Hongyan Li

Annals of diagnostic pathology 34:103-109 PubMed29661714

2018

microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia.

Applications

Unspecified application

Species

Unspecified reactive species

Esmerina Tili,Louisa Mezache,Jean-Jacques Michaille,Vicky Amann,James Williams,Paige Vandiver,Maria Quinonez,Paolo Fadda,Adel Mikhail,Gerard Nuovo

Cell death & disease 7:e2287 PubMed27362805

2016

Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

R S Al-Lamki,W Lu,P Manalo,J Wang,A Y Warren,A M Tolkovsky,J S Pober,J R Bradley

The journals of gerontology. Series A, Biological sciences and medical sciences 60:976-83 PubMed16127099

2005

Aging sustains the hypertrophy-associated elevation of apoptotic suppressor X-linked inhibitor of apoptosis protein (XIAP) in skeletal muscle during unloading.

Applications

Unspecified application

Species

Unspecified reactive species

Parco M Siu,Emidio E Pistilli,Michael J Ryan,Stephen E Alway

American journal of physiology. Regulatory, integrative and comparative physiology 289:R1015-26 PubMed15919734

2005

Apoptotic responses to hindlimb suspension in gastrocnemius muscles from young adult and aged rats.

Applications

Unspecified application

Species

Unspecified reactive species

Parco M Siu,Emidio E Pistilli,Stephen E Alway

Journal of applied physiology (Bethesda, Md. : 198 99:204-9 PubMed15774698

2005

Response of XIAP, ARC, and FLIP apoptotic suppressors to 8 wk of treadmill running in rat heart and skeletal muscle.

Applications

Species

Rat

Parco M Siu,Randall W Bryner,Zsolt Murlasits,Stephen E Alway

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FLIP antibody

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB6144)

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB6144)

Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (AB6144)

Western blot - Anti-FLIP antibody (AB6144)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise