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AB314748

Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free

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Rabbit Recombinant Monoclonal FLIP antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CASH, CASP8AP1, CLARP, MRIT, CFLAR, CASP8 and FADD-like apoptosis regulator, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, Inhibitor of FLICE, MACH-related inducer of toxicity, Usurpin, Casper, c-FLIP, FLAME-1, I-FLICE

6 Images
Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)
  • WB

Supplier Data

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)

This data was developed usingab314747, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the higher MW band at approximately 75 kDa is unknown. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FLIP-L antibody [EPR27008-110] (<a href='/en-us/products/primary-antibodies/flip-l-antibody-epr27008-110-ab314747'>ab314747</a>) at 1/1000 dilution

All lanes:

HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,37 kDa

false

Exposure time: 26s

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)
  • WB

Supplier Data

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)

This data was developed usingab314747, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : NAMALWA (PMID : 15078899). The 55kDa and 25kDa bands represent full-length FLIP (FLIP-L) and short-form FLIP (FLIP-S) (PMID : 15078899). The identity of the higher MW band at approximately 75 kDa is unknown. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FLIP-L antibody [EPR27008-110] (<a href='/en-us/products/primary-antibodies/flip-l-antibody-epr27008-110-ab314747'>ab314747</a>) at 1/1000 dilution

Lane 1:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

NAMALWA (human burkitt's lymphoma b lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,37 kDa

false

Exposure time: 114s

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)
  • WB

Supplier Data

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)

This data was developed usingab314747, the same antibody clone in a different buffer formulation.#Blocking and diluting buffer and concentration : 5% NFDM/TBST#Low expression : NAMALWA (PMID : 15078899).##The 55kDa and 25kDa bands represent full-length FLIP (FLIP-L) and short-form FLIP (FLIP-S) (PMID : 15078899). ##The identity of the higher MW band at approximately 75 kDa is unknown. ##Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.#In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.#

All lanes:

Western blot - Anti-FLIP-L antibody [EPR27008-110] (<a href='/en-us/products/primary-antibodies/flip-l-antibody-epr27008-110-ab314747'>ab314747</a>) at 1/1000 dilution

Lane 1:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate 20 µg

Lane 2:

NAMALWA (human burkitts lymphoma b lymphocyte) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 114s

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)
  • WB

Supplier Data

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)

This data was developed usingab314747, the same antibody clone in a different buffer formulation.#Blocking and diluting buffer and concentration : 5% NFDM/TBST#Low expression : Daudi ( PMID : 15078899).##The identity of the higher MW band at approximately 75 kDa is unknown. ##Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.#In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.#

All lanes:

Western blot - Anti-FLIP-L antibody [EPR27008-110] (<a href='/en-us/products/primary-antibodies/flip-l-antibody-epr27008-110-ab314747'>ab314747</a>) at 1/1000 dilution

Lane 1:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate 20 µg

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 114s

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)
  • WB

Supplier Data

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)

This data was developed usingab314747, the same antibody clone in a different buffer formulation.#Blocking and diluting buffer and concentration : 5% NFDM/TBST#The identity of the higher MW band at approximately 75 kDa is unknown. ##Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.#In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.#

All lanes:

Western blot - Anti-FLIP-L antibody [EPR27008-110] (<a href='/en-us/products/primary-antibodies/flip-l-antibody-epr27008-110-ab314747'>ab314747</a>) at 1/1000 dilution

All lanes:

HDLM-2 (human Hodgkin lymphoma) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 26s

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)
  • WB

Supplier Data

Western blot - Anti-FLIP-L antibody [EPR27008-110] - BSA and Azide free (AB314748)

This data was developed using ab314747, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : Daudi ( PMID : 15078899). The identity of the higher MW band at approximately 75 kDa is unknown. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FLIP-L antibody [EPR27008-110] (<a href='/en-us/products/primary-antibodies/flip-l-antibody-epr27008-110-ab314747'>ab314747</a>) at 1/1000 dilution

Lane 1:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 20 µg

Lane 2:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,37 kDa

false

Exposure time: 114s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27008-110

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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SDS-PAGE - Recombinant Human FLIP protein (denatured) (Tag Free) (AB111628)

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Western blot - Anti-XIAP antibody [EPR22189-113] (AB229050)

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RabMAb|Recombinant|20ul selling size

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

  • 3
  • 3
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Reactivity data

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Product details

ab314748 is the carrier-free version of ab314747.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FLIP-L also known as FLICE-like inhibitory protein-long is an important regulator in apoptosis. It has a molecular mass of approximately 55 kDa. FLIP-L is expressed in various tissues especially in lymphoid tissues and the spleen. It contains two death effector domains (DEDs) that hinder the protease activity of initiator caspases important components driving cell death pathways. By inhibiting caspase-8 FLIP-L prevents the initiation of apoptosis making it an important player in the control of cell death.
Biological function summary

FLIP-L inhibits apoptosis by interacting with the death-inducing signaling complex (DISC). It competes with procaspase-8 for binding within this complex reducing the activation of caspase-8 and subsequent apoptotic signals. This inhibition plays an important role in the regulation of immune responses and cancer progression. Through its involvement in apoptosis and immune signaling FLIP-L modulates cellular survival especially in the immune cell lineage where its function prevents excessive cell death.

Pathways

FLIP-L operates within the extrinsic apoptosis pathway and exhibits strong interactions with components like FADD (Fas-associated protein with death domain). It influences the balance between cell survival and death interacting with important players like caspases and TNF receptor signaling pathways. By modulating these interactions FLIP-L determines whether a cell follows a path towards apoptosis or survival reflecting its importance in maintaining cellular homeostasis.

FLIP-L has connections to cancer and autoimmune conditions. In many cancers elevated FLIP-L levels correlate with resistance to apoptosis hindering effective cancer treatments. Furthermore FLIP-L impacts autoimmune diseases by altering immune cell apoptosis leading to unchecked immune activity. Proteins such as Bcl-2 and caspase-8 are often discussed in connection with FLIP-L when analyzing its role in disease. By affecting these proteins FLIP-L contributes to the persistence and progression of various disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells (PubMed : 31046799). Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
See full target information CFLAR
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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